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[(properties.)-310.5(Collagen)-311.9(can)-310.9(induce)-308.6(the)-309.9(mineral)-311(deposition,)-309.6(and)]TJ T* [(its)-320.1(surface)-322(contains)-322.2(sites)-317.6(that)-322.6(promote)-321.2(osteoblast)-318.6(adsorption)]TJ T* [(and)-517.1(mineral)-518.3(deposition.)-516.9(Moreover,)-516.5(it)-515.1(can)-518.2(effectively)-518.2(en-)]TJ 0 -1.1174 TD [(hance)-366.1(the)-361.7(mineralization)-361.7(process)-366.7(and)-361.6(develop)-361.5(new)-362.4(bone)-362.7(in)]TJ 0 -1.1116 TD [(the)-379(implanted)-376.7(articial)-376.6(bone)-374.2(scaffold.)]TJ 7.3823 0 0 6.5614 214.9228 83.5654 Tm (16)Tj 9.843 0 0 9.843 225.9779 79.2 Tm [(This)-374.2(kind)-378.9(of)-370.8(poly-)]TJ -16.6571 -1.1116 TD [(mer)-558.6(can)-552.8(retain)-556.3(but)-556.4(does)-558.3(not)-550.6(occlu)-8(de)-550.5(the)-557.5(osteoinductive)]TJ 0 -1.1174 TD [(calcium)-312.2(phosphate)-305.9(component)-307.4(and)-304(has)-309.5(osteoconductive)-309.4(and)]TJ 25.504 67.8551 TD 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[(and)-363(o)5.6(st)5.5(eo)5.6(gen)5.6(i)-.2(c)-364.1(d)5.6(if)5.7(fer)5.7(e)-1.3(n)5.6(t)-.2(i)5.5(a)-1.3(ti)5.5(on)5.6(,)-365.3(w)4.8(ere)-364.1(c)4.5(ompared)-363(a)-1.3(n)5.6(d)-363(ev)5.6(alu)5.6(-)]TJ T* .0117 Tc [(ated.)-337.3(T)3.6(he)-336.1(ap)4.8(pl)4.7(icat)4.7(ion)-335(o)-.9(f)-334.9(t)-1(wo)-340.7(h)4.8(y)-.9(d)4.8(r)-.9(o)4.8(g)-.9(els)-340.5(u)4.8(sed)-340.7(a)3.7(s)-340.5()51()4.9(b)-.9(i)4.7(o)-.9(i)4.7(nks)5()51()]TJ T* .012 Tc [(in)-323.2(LT-3DP)-323(a)4(nd)-317.4(th)5.1(ese)-318.6(b)-.6(i)5(o)-.6(l)5(ogi)5(ca)]TJ 12.9075 0 TD .011 Tc [(l)-324.3(p)4.1(ro)4.1(perti)4(e)-2.8(s)-318.2(w)-2.5(ere)-325.3(c)3(ompared)]TJ -12.9075 -1.1174 TD .0108 Tc [(a)-3(n)-1.8(d)-330.1(e)2.8(va)-3(luated.)]TJ /F8 1 Tf 8.9663 0 0 8.9663 313.0582 537.4487 Tm 0 Tc [(Materials)-339(and)-331.9(Methods)]TJ /F11 1 Tf 0 -1.8336 TD [(Preparation)-339(of)-330.4(DBB)]TJ /F4 1 Tf 9.843 0 0 9.843 323.0361 504.5668 Tm [(DBB)-369.1(was)-368(prepared)-369.3(by)-365.1(calcining)-370.9(bovine)-365(cancellous)-372.8(bone)]TJ -1.0137 -1.1116 TD [(following)-345.2(the)-338.7(method)-337.4(of)-336.2(Wang.)]TJ 7.3823 0 0 6.5614 440.7306 497.9904 Tm (19)Tj 9.843 0 0 9.843 451.4456 493.6251 Tm [(In)-336.2(brief,)-338.5(the)-338.7(bovine)-342(bone)]TJ -14.0594 -1.1116 TD [(was)-333.4(cut)-332.9(into)-333(small)-330.5(cubes,)-333.6(and)-332.8(successively)-334.3(being)-335.1(incubated)]TJ 0 -1.1174 TD [(in)-301.8(H)]TJ 7.3823 0 0 6.5614 330.8031 470.0408 Tm (2)Tj 9.843 0 0 9.843 334.4881 471.6849 Tm (O)Tj 7.3823 0 0 6.5614 341.5747 470.0408 Tm (2)Tj 9.843 0 0 9.843 348.2645 471.6849 Tm [(solution)-302.7(and)-298.2(stewed)-303.5(in)-301.8(a)-301.8(NaOH)-301.9(solution)-302.7(to)-301.8(remove)]TJ -3.5768 -1.1116 TD [(collagen)-444.5(and)-436.4(proteins.)-439.7(The)-437.6(bone)-431.8(cubes)-439.6(were)-439.5(then)-437.7(washed)]TJ T* .013 Tc [(w)-6.2(i).3(t)-5.5(h)-368.2(wa)-6.5(te)-6.5(r,)-370.6(im)-6.6(mer)-5.3(se)-6.5(d)-368.2(i).3(n)-374(H)]TJ 7.3823 0 0 6.5614 426.3306 448.1573 Tm 0 Tc (3)Tj 9.843 0 0 9.843 430.1857 449.8015 Tm .0125 Tc (PO)Tj 7.3823 0 0 6.5614 442.9983 448.1573 Tm 0 Tc (4)Tj 9.843 0 0 9.843 450.4251 449.8015 Tm .0151 Tc [(solution,)-368.5(a)1.3(nd)-366.1(he)-4.4(ated,)-368.5(r)-3.2(e-)]TJ -13.9558 -1.1174 TD 0 Tc [(spectively.)-525(This)-518.2(was)-517.8(followed)-517.9(by)-514.8(washing)-516.5(with)-518.1(absolute)]TJ 0 -1.1116 TD [(ethyl)-277.6(alcohol)-269.5(to)-273(remove)-275.1(moisture.)-268.1(After)-278.3(that,)-268.4(the)-275.3(bone)-270.5(cubes)]TJ T* [(were)-416.5(air-dried)-415.6(and)-407.6(heate)-9.1(d)-410(u)0(p)-411.1(t)0(o)-405.5(1000)]TJ /F6 1 Tf 15.7298 0 TD ()Tj /F4 1 Tf .3917 0 TD [(C.)-414.5(Finally)-412.3(the)-413.5(bone)]TJ -16.1214 -1.1174 TD [(cubes)-249.5(were)-243.7(ground)-242.7(and)-246.4(ltered)-246.2(through)-244(a)-244.2(120-screen)-244.6(cloth)-243.1(to)]TJ 0 -1.1116 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[(The)-489.5(hydrogel)-482.4(scaffolds)-484.2(were)-485.6(fabricated)-488.2(by)-480.2(layer-by-layer)]TJ /F8 1 Tf 8.9663 0 0 8.9663 62.022 749.6502 Tm (1262)Tj 50.2102 0 TD [(LI)-331.6(ET)-338.7(AL.)]TJ ET endstream endobj 52 0 obj <>stream /GS1 gs BT /F4 1 Tf 9.843 0 0 9.843 59.7543 725.1588 Tm 0 0 0 rg 0 Tc 0 Tw [(deposition,)-465.1(and)-465.2(a)-463(1)0(5)]TJ /F6 1 Tf 8.8815 0 TD ()Tj /F4 1 Tf .72 0 TD (15)Tj /F6 1 Tf 1.1635 0 TD ()Tj /F4 1 Tf .72 0 TD .1681 Tc [(5m)168.1(m)]TJ 7.3823 0 0 6.5614 194.7401 729.5242 Tm 0 Tc (3)Tj 9.843 0 0 9.843 203.0173 725.1588 Tm [(architecture)-468.6(was)-465.9(gotten)]TJ -14.5548 -1.1174 TD [(\(Fig.)-491.6(1A,)-484.6(B\).)-490.4(The)-489.5(parameters)-489.1(of)-491.7(the)-488.4(3D)-486.9(Bioplotter)-491.8(were)]TJ 0 -1.1116 TD [(determined)-401.9(after)-393.8(repeated)-399.5(printing)-395.1(experimentations)-398.2(to)-394(en-)]TJ T* [(sure)-356.7(the)-355.9(consistency)-361.1(and)-350(repea)-9(tability)-356.2(of)-353.5(the)-355.9(3D)-354.4(bioprinted)]TJ T* [(constructions.)-336.8(Related)-335(parameters)-339.3(are)-333.9(presented)-335.8(in)-330.6(Table)-337.5(1.)]TJ 0 -1.1174 TD [(When)-434.1(each)-434.1(printed)-429.6(scaffold)-437.2(was)-431.4(completed,)-433(freeze-drying)]TJ 0 -1.1116 TD [(was)-471.7(conducted)-468.6(to)-463.1(obtain)-468.8(the)-465.4(freeze-dried)-474(scaffolds.)-470.4(After)]TJ T* [(that,)-424(the)-425(scaffolds)-426.6(were)-428(crosslinked)-423.2(with)-426(0.2%)-422.5(glutaralde-)]TJ 0 -1.1174 TD [(hyde)-512.4(solution)-510.1(\(80%)-512.3(ethanol)-517.2(aqueous)-510.9(solution\))-511.2(for)-510.1(2)-168.1(h)-507.9(at)]TJ 0 -1.1116 TD [(room)-460.6(temperature,)-459.4(then)-455(rinsed)-456.9(in)-457.3(deionized)-457.2(water)-458.1(several)]TJ T* [(times)-468.7(to)-468.9(remove)-470.9(residual)-466.2(glutaraldehyde.)-473.1(The)-472.2(crosslinked)]TJ 0 -1.1174 TD [(scaffolds)-426.6(were)-422.3(put)-418.1(into)-425.1(freeze-dryer)-422.1(again)-422.7(to)-422.8(get)-419.3(the)-425(nal)]TJ 0 -1.1116 TD [(specimen.)-281.9(The)-270.6(sterile)-280.9(operations)-272.6(were)-278.3(carried)-276.2(out)-274.1(during)-271.7(the)]TJ T* (processes.)Tj /F11 1 Tf 8.9663 0 0 8.9663 59.7543 559.6723 Tm [(SEM)-338.9(of)-330.4(the)-334.8(3D)-336(bioprinting)-332.9(scaffold)]TJ /F4 1 Tf 9.843 0 0 9.843 69.7323 543.2313 Tm [(Th)19.6(e)-273(f)0(r)20.9(e)0(e)12.8(z)0(e)18.5(-)0(d)20.9(r)0(i)20.7(e)0(d)-262.5(s)0(c)19.9(a)0(f)14(f)0(o)20.9(l)0(d)20.7(s)-271.6(we)18.8(re)-262.5(co)13.9(at)19.5(ed)-262.5(wi)19.8(th)-261.5(Au)-262.3(an)19.7(d)-271.8(i)0(n)15(v)0(e)19.7(s)0(-)]TJ -1.0137 -1.1174 TD [(ti)14.8(ga)19.7(te)19.6(d)-600.1(w)0(i)19.8(t)0(h)-589.8(S)0(E)19.8(M)-595.2(at)-591(a)-601.3(m)0(a)18.4(g)0(n)20.8(i)0()20.9(c)0(a)18.5(t)0(i)14.8(o)0(n)-583.9(b)0(e)13.9(t)0(w)19.8(e)0(e)18.5(n)-600.1(10)20.8(0)]TJ /F6 1 Tf 22.1576 0 TD ()Tj /F4 1 Tf .7084 0 TD [(an)19.7(d)]TJ -22.866 -1.1116 TD [(20)15.1(00)]TJ /F6 1 Tf 2.1311 0 TD ()Tj /F4 1 Tf .7084 0 TD [(as)-158.7(th)20.7(e)-163.5(m)0(e)18.4(t)0(h)20.7(o)0(d)-157.7(o)0(f)-157.7()62.3(P)15.3(ro)20.9(pe)19.7(rt)20.7(ie)19.6(s)-167.9(o)0(f)-157.7(h)0(y)20.8(d)0(r)20.9(o)0(x)20.8(y)0(a)13.9(p)0(a)19.7(t)0(i)20.6(t)0(e)19.6()62.3()-168.1(a)0(b)19.7(o)0(v)20.8(e)0(.)]TJ /F11 1 Tf 8.9663 0 0 8.9663 59.7543 498.2739 Tm [(Fourier)-336.3(transform)-335.5(infrared)-332.2(spectroscopy)-335.1(analysis)]TJ /F4 1 Tf 9.843 0 0 9.843 69.7323 481.8329 Tm [(The)-414.6(chemical)-418.1(composition)-412.1(of)-411.1(the)-413.5(scaffolds)-415.1(was)-414.1(investi-)]TJ -1.0137 -1.1116 TD [(gated)-520.6(by)-509.1(Fourier)-515.6(transform)-519(infrared)-518(spectroscopy)-517.5(\(FTIR;)]TJ 0 -1.1174 TD [(Spectrum)-369.4(GX\).)-359.8(Each)-365(of)-365(samples)-362.3(was)-362.2(ground)-363.7(together)-368.5(with)]TJ 0 -1.1116 TD [(KBr)-293.2(at)-285.7(a)-290.2(rate)-289(about)-289(1:20)-292.5(in)-284.6(an)-291.3(agate)-291.3(mortar)-285.5(and)-292.4(compressed)]TJ T* [(to)-324.9(tablets.)-330.4(The)-328.2(spectra)-327.9(ranges)-325.5(were)-330.1(set)-326.9(as)-325.7(4000)-327(to)-324.9(400)-164.6(cm)]TJ /F6 1 Tf 7.3823 0 0 6.5614 291.118 442.3747 Tm (-)Tj /F4 1 Tf .5529 0 TD (1)Tj 9.843 0 0 9.843 59.7543 427.0109 Tm [(with)-426(a)-428.5(resolution)-427(of)-428.4(4)-162.4(c)0(m)]TJ /F6 1 Tf 7.3823 0 0 6.5614 164.8062 431.3763 Tm (-)Tj /F4 1 Tf .5529 0 TD (1)Tj 9.843 0 0 9.843 172.5732 427.0109 Tm [(,)-423.9(and)-424.9(the)-430.8(scan)-427(time)-427.5(was)-425.6(about)]TJ -11.4618 -1.1116 TD [(100)-170.3(s.)-332.6(Pure)-333.7(collagen)-335.1(sponge)-335.9(served)-337(as)-337.2(a)-330.6(reference.)]TJ /F11 1 Tf 8.9663 0 0 8.9663 59.7543 393.0519 Tm [(X-ray)-334.9(diffraction)-340(analysis)]TJ /F4 1 Tf 9.843 0 0 9.843 69.7323 376.6109 Tm [(The)-460.7(scaffolds)-461.2(lyophilized)-458.5(were)-456.8(cut)-459.6(into)-459.7(small)-457.2(cubes)-456.9(at)]TJ -1.0137 -1.1116 TD (20)Tj /F6 1 Tf 1.1692 0 TD ()Tj /F4 1 Tf .72 0 TD (20)Tj /F6 1 Tf 1.1635 0 TD ()Tj /F4 1 Tf .72 0 TD [(2)-168.1(mm,)-503.4(and)-505.6(gently)-503.4(attened)-507.7(before)-504.2(analysis.)-509.8(The)]TJ -3.7726 -1.1174 TD [(crystal)-453.6(phase)-445.4(compositions)-453.4(of)-445.7(each)-445.6(scaffold)-448.7(were)-451.1(investi-)]TJ 0 -1.1116 TD [(gated)-319(by)-307.5(X-ray)-318.5(diffraction)-314.2(\(XRD,)-315.9(SmartLab)-318.8(X,)-310.7(Japan\).)-317.4(The)]TJ T* [(materials)-371.9(XRD)-368.9(spectra)-368.2(were)-370.4(taken)-370.8(using)-362.5(nickel-ltered)-371.7(Cu)]TJ /F5 1 Tf 0 -1.1174 TD (K)Tj /F10 1 Tf .6681 0 TD (a)Tj /F4 1 Tf .9331 0 TD [(radiation)-387(at)-383.6(40)-163.5(kV)-383.2(and)-384.6(200)-164.6(mA.)-382.1(Spectra)-385.5(were)-381.9(recorded)]TJ -1.6012 -1.1116 TD [(from)-282(2)]TJ /F10 1 Tf 2.7301 0 TD (y)Tj /F6 1 Tf .6624 0 TD (=)Tj /F4 1 Tf .72 0 TD (5)Tj /F6 1 Tf .5011 0 TD ()Tj /F4 1 Tf .3974 0 TD [(to)-278.8(90)]TJ /F6 1 Tf 2.0562 0 TD ()Tj /F4 1 Tf .3974 0 TD [(at)-280(a)-278.7(scanning)-281.8(speed)-278.3(of)-278.6(15)]TJ /F6 1 Tf 10.2465 0 TD ()Tj /F4 1 Tf .3917 0 TD [(/min)-282.4(and)-280.9(a)-278.7(step)]TJ -18.1028 -1.1116 TD [(size)-346.5(of)-342(0.02)]TJ /F6 1 Tf 4.8324 0 TD ()Tj /F4 1 Tf .3974 0 TD [(.)-343.3(nHA)-344.9(and)-344.3(DBB)-340.3(powder)-348.4(served)-342.8(as)-343(a)-347.8(reference)]TJ -5.2298 -1.1174 TD [(and)-338.5(was)-333.4(heated)-338.5(to)-330.6(100)]TJ /F6 1 Tf 9.2443 0 TD ()Tj /F4 1 Tf .3917 0 TD [(C)-336.2(for)-331.5(12)-169.2(h)-329.4(before)-337.2(use.)]TJ /F11 1 Tf 8.9663 0 0 8.9663 59.7543 265.8897 Tm [(Mechanical)-339.2(properties)-329.8(of)-336.7(scaffolds)]TJ /F4 1 Tf 9.843 0 0 9.843 69.7323 249.4488 Tm [(Th)19.6(e)-267.2(Y)0(o)20(u)0(n)15.1(g)0()20.9(s)-265.8(mo)19.6(du)20.8(lu)20.7(s)-265.8(o)0(f)-261.4(t)0(h)20.7(e)-267.2(th)20.7(re)19.7(e)-267.2(g)0(r)20.9(o)0(u)15.1(p)0(s)-255.4(o)0(f)-255.6(s)0(c)19.9(a)0(f)19.7(f)0(o)15.1(l)0(d)-255.8(w)0(e)18.8(r)0(e)]TJ -1.0137 -1.1116 TD [(de)13.9(te)19.5(rm)19.6(in)20.7(ed)-654.2(us)21(in)20.7(g)-663.5(a)-658.9(Un)14.2(iv)20.7(er)19.7(sa)19.9(l)-663.6(T)0(e)18.5(s)0(t)20.9(i)0(n)20.7(g)-663.5(Ma)18.8(ch)19.7(in)15(e)-658.9(\()0(I)20.9(n)0(s)15.3(t)0(r)20.7(o)0(n)]TJ T* [(30)15.1(0D)20(X,)-368.3(US)14.4(\).)-373.1(Th)19.6(e)-382.4(d)0(i)20.7(m)0(e)18.4(n)0(s)21(i)0(o)20.7(n)0(s)-376.4(o)0(f)-370.8(e)0(a)18.5(c)0(h)-372(s)0(p)21(e)0(c)18.5(i)0(m)13.7(e)0(n)-372(w)0(e)18.8(r)0(e)-371.9(m)0(e)18.4(a)0(-)]TJ 0 -1.1174 TD [(su)15.3(re)19.7(d)-536.7(u)0(s)21(i)0(n)20.7(g)-536.7(a)-537.9(v)0(e)19.7(r)0(n)20.9(i)0(e)19.6(r)-536.7(ca)18.5(li)20.6(pe)19.7(r)-536.7(b)0(e)19.7(f)0(o)20.9(r)0(e)-527.5(d)0(e)19.7(t)0(e)13.8(c)0(t)19.5(i)0(o)20.7(n)0(.)-522.9(T)0(h)19.6(e)0(n)13.9(,)-533.3(th)20.7(e)]TJ 0 -1.1116 TD [(sc)14.1(af)19.7(fo)20.9(ld)20.7(s)-254.3(w)0(e)18.8(r)0(e)-251(p)0(l)20.7(a)0(c)18.5(e)0(d)-245.3(o)0(n)-244.1(t)0(h)20.7(e)-255.7(de)19.7(te)13.8(ct)19.5(in)20.7(g)-254.5(p)0(l)20.7(a)0(t)19.5(f)0(o)20.9(r)0(m)-245.3(a)0(n)13.9(d)-254.5(lo)20.7(ad)19.7(ed)-245.3(at)]TJ T* [(th)15(e)-370.9(r)0(a)19.7(t)0(e)-366.3(o)0(f)-365(1)-156.6(mm)24.1(/m)19.5(in)-365.2(un)20.8(ti)14.8(l)-369.8(t)0(h)20.7(e)-376.6(sc)19.9(af)19.7(fo)20.9(ld)15(s)-369.5(c)0(r)19.7(u)0(s)15.3(h)0(e)19.7(d)0(.)-361.6(T)0(h)19.6(e)-376.6(fo)20.9(rc)19.7(e)]TJ 0 -1.1174 TD [(an)13.9(d)-335.2(d)0(i)20.7(s)0(p)21(l)0(a)19.6(c)0(e)18.5(m)0(e)18.4(n)0(t)-330.6(d)0(a)19.7(t)0(a)-326(w)0(e)18.8(r)0(e)-325.9(r)0(e)19.7(c)0(o)13.9(r)0(d)20.9(e)0(d)-325.9(t)0(o)-324.9(g)0(e)19.7(n)0(e)13.9(r)0(a)19.7(t)0(e)-326(t)0(h)20.7(e)-336.3(st)20.9(re)14(ss)21.2()]TJ 0 -1.1116 TD [(st)15.1(ra)19.7(in)-480.4(cu)19.7(rv)20.9(es)19.9(.)-487.2(B)0(y)-481.3(m)0(e)18.4(a)0(s)19.9(u)0(r)20.9(i)0(n)15(g)-484.9(th)20.7(e)-491.8(s)0(l)20.9(o)0(p)20.8(e)-491.8(of)-480.2(th)20.7(e)-491.8(s)0(t)20.9(r)0(e)19.7(s)0(s)21.2()0(s)15.3(t)0(r)20.7(a)0(i)19.5(n)]TJ T* [(cu)13.9(rv)20.9(e)-388.2(i)0(n)-376.7(t)0(h)20.7(e)-388.2(el)19.6(as)19.9(ti)20.6(c)-388.2(r)0(e)14(g)0(i)20.7(o)0(n)20.8(,)-383.6(Yo)14.2(un)20.8(g)20.9(s)-386.8(m)0(o)19.6(d)0(u)20.8(l)0(u)20.7(s)-392.6(co)19.7(ul)20.7(d)-387(b)0(e)-377.7(c)0(a)18.5(l)0(-)]TJ 0 -1.1174 TD [(cu)13.9(la)19.5(te)19.6(d.)-327.1(Ea)18.5(ch)-331.7(gr)15.1(ou)20.8(p)-340.9(w)0(a)18.8(s)-340.7(de)19.7(te)19.6(ct)19.5(ed)-331.7(v)21(e)-342.1(t)0(i)14.8(m)15(es)14.1(,)-337.5(a)0(n)19.7(d)-340.9(th)20.7(e)-342.1(r)0(e)19.7(s)0(u)15.3(l)0(t)20.6(s)]TJ 0 -1.1116 TD [(ar)14(e)-319(d)0(i)14.9(s)0(p)21(l)0(a)19.5(y)0(e)19.7(d)-323.6(in)-313.3(th)20.7(e)-324.8(f)0(o)20.9(r)0(m)-314.4(o)0(f)-313.2(m)0(e)18.4(a)0(n)]TJ /F6 1 Tf 13.9385 0 TD ()Tj /F4 1 Tf .7084 0 TD [(st)15.1(an)19.7(da)19.7(rd)-313.2(de)19.7(vi)20.7(at)13.8(io)20.7(n.)]TJ /F11 1 Tf 8.9663 0 0 8.9663 59.7543 116.8441 Tm [(Evaluation)-339.5(of)-330.4(the)-334.8(biological)-338.3(characteristics)]TJ 0 -1.2203 TD [(of)-336.7(the)-334.8(bioprinting)-332.9(materials)]TJ /F2 1 Tf 1.1128 -1.8336 TD [(Cell)-538.6(culture.)]TJ /F4 1 Tf 9.843 0 0 9.843 129.1464 89.4614 Tm [(Human)-515.6(bone)-518.2(marrow-derived)-522.1(mesenchy-)]TJ -7.0499 -1.1174 TD [(mal)-409(stem)-409.9(cells)-411.1(\(hBMSCs\))-414.6(were)-405(purchased)-416.4(from)-408.7(ScienCell)]TJ 0 -1.1116 TD [(\(ScienCell\))-431.5(and)-419.2(cultured)-426.1(in)-422.8(10)-169.2(cm)-425(dishes)-422.2(with)-426(Dulbeccos)]TJ T* [(modied)-246.2(Eagles)-253(medium)-246.5(\(DMEM\))-246.6(supplemented)-248.4(with)-247.4(10%)]TJ 25.5097 67.9242 TD [(\(v/v\))-293.6(fetal)-290.3(bovine)-295.9(serum)-289.9(\(FBS\))-294.1(in)-290.3(incubator)-294.7(\(37)]TJ /F6 1 Tf 19.3008 0 TD ()Tj /F4 1 Tf .3974 0 TD [(C,)-287.8(5%)-291.2(CO)]TJ 7.3823 0 0 6.5614 546.2361 723.5148 Tm (2)Tj 9.843 0 0 9.843 310.8472 714.1605 Tm [(and)-505.6(100%)-500.8(relative)-508(humidity\).)-510.3(The)-506.8(medium)-505.7(was)-506.2(changed)]TJ 0 -1.1116 TD [(three)-244.1(times)-244.1(a)-244.2(week)-243.8(until)-242(passag)-8.5(e)-238.4(3)-243(\(P3\).)-243.8(After)-243.8(being)-248.7(digested)]TJ T* [(and)-597.7(counted,)-606.9(the)-603.6(cell)-600.2(suspension)-605(was)-604.2(concentrated)-606.8(and)]TJ T* [(suspended)-340.2(in)-336.4(DMEM)-342.4(\(10%)-339.5(FBS\))-339.2(to)-336.4(adjust)-341.9(the)-338.7(cell)-335.3(number)]TJ 0 -1.1174 TD [(to)-255.8(6)]TJ /F6 1 Tf 1.7049 0 TD ()Tj /F4 1 Tf .7142 0 TD (10)Tj 7.3823 0 0 6.5614 344.5227 674.7021 Tm (7)Tj 9.843 0 0 9.843 348.2078 670.3369 Tm [(/mL.)-257(After)-261(placing)-258(the)-258(sterile)-257.9(3D)-262.3(printing)-256.8(scaffolds)]TJ -3.7956 -1.1116 TD [(in)-451.6(a)-457.3(24-well)-459.2(culture)-453.8(plate)-457.3(and)-459.5(wetting)-453.6(the)-459.6(materials)-458.3(with)]TJ T* [(proliferation)-351.1(medium)-350.2(\(PM\))-350.7(beforehand,)-351.9(each)-353.5(scaffold)-350.8(sam-)]TJ 0 -1.1174 TD [(ple)-309.9(was)-316.2(seeded)-315.2(with)-316.6(100)]TJ /F10 1 Tf 10.2062 0 TD (m)Tj /F4 1 Tf .5529 0 TD [(L)-313.3(o)0(f)-313.2(cell)-312.3(suspension.)-314.7(After)-318.6(4)-168.1(h)-312.1(of)]TJ -10.7591 -1.1116 TD [(cell)-271.9(adhesion)-276(to)-267.3(the)-275.3(scaffold)-270.2(in)-273(incubator,)-275.1(900)]TJ /F10 1 Tf 18.6442 0 TD (m)Tj /F4 1 Tf .5529 0 TD [(L)-273(o)0(f)-267.1(DMEM)]TJ -19.1971 -1.1116 TD [(\(10%)-391.3(FBS\))-396.7(was)-396.8(added)-399.5(to)-394(each)-399.6(well.)-396(The)-397.3(medium)-396.3(was)-396.8(re-)]TJ 0 -1.1174 TD [(placed)-332.8(every)-336.1(other)-335.1(day.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 320.8251 581.2723 Tm [(Immunouorescence)-280.7(staining.)]TJ /F4 1 Tf 9.843 0 0 9.843 449.4613 581.2723 Tm [(The)-293.7(immunouorescence)]TJ -14.0825 -1.1116 TD [(staining)-263.6(of)-261.3(hBMSCs)-268.4(cytoskeleton)-270.3(was)-264.3(conducted.)-264.6(After)-266.8(24)-163.5(h)]TJ T* [(of)-376.5(incubation)-376.6(on)-376.6(each)-376.5(scaffold,)-383.1(the)-373.2(cells)-382.3(were)-376.2(xed)-379.8(in)-376.7(4%)]TJ 0 -1.1174 TD [(paraformaldehyde)-600.7(for)-602.2(10)-163.5(min)-597.9(at)-596.7(room)-598.8(temperature,)-603.4(per-)]TJ 0 -1.1116 TD [(meabilized)-552.9(with)-546.9(0.1%)-543.5(TritonTMX-100)-554.3(PBS)-545.4(solution)-550.4(for)]TJ T* [(5)-168.1(min,)-324.9(and)-327(blocked)-327(using)-328(1%)-331.6(bovine)-324.7(serum)-330.2(albumin)-327.2(\(BSA\))]TJ 0 -1.1174 TD [(for)-343.1(30)-169.2(min)-344.5(at)-343.3(room)-345.4(temperature.)-349.9(Then,)-350(the)-344.4(cells)-347.7(were)-347.4(incu-)]TJ 0 -1.1116 TD [(bated)-463(with)-466.3(FITC-phalloidin)-469.5(\(Sigma-Aldrich\))-464.6(at)-464.3(a)-468.8(concen-)]TJ T* [(tration)-642.8(of)-641.5(5)]TJ /F10 1 Tf 5.4026 0 TD (m)Tj /F4 1 Tf .5529 0 TD [(g/mL)-640.5(with)-644.9(each)-641.5(well)-646(0.5)-166.9(mL)-638.2(for)-642.6(30)-169.2(min,)]TJ -5.9555 -1.1116 TD [(followed)-327.9(by)-319(incubation)-330.5(in)-324.9(DAPI)-322.7(\(5)]TJ /F10 1 Tf 14.526 0 TD (m)Tj /F4 1 Tf .5529 0 TD [(g/mL\))-324.8(for)-325.8(5)-162.4(min)-327.2(in)-324.9(the)]TJ -15.0789 -1.1174 TD [(dark)-282(at)-280(room)-282.1(temperature.)-286.6(Each)-284.4(incubation)-284.4(was)-281.6(followed)-287.5(by)]TJ 0 -1.1116 TD [(three)-347.7(times)-347.8(of)-347.7(PBS)-349.6(washing.)-352.9(Confocal)-352.1(Laser)-348.6(Scanning)-350.9(Mi-)]TJ T* [(croscope)-547.7(\(CLSM,)-541.8(Olympus)-545.4(IX71,)-544.3(Fluoview,)-544.3(Japan\))-550.1(was)]TJ 0 -1.1174 TD [(used)-333.7(to)-330.6(observe)-338.1(the)-332.9(images.)]TJ 7.3823 0 0 6.5614 422.929 443.2251 Tm (21)Tj /F2 1 Tf 8.9663 0 0 8.9663 320.8251 415.5022 Tm [(Vinculin)-387.3(staining.)]TJ /F4 1 Tf 9.843 0 0 9.843 398.6645 415.5022 Tm -.0207 Tc [(T)-20.7(h)-1.1(e)-391.6(v)-20.7(in)-20.7(c)-6.8(u)-20.7(li)-20.7(n)-380.1(s)-20.7(ta)-20.7(i)-6.9(n)-20.7(in)-20.7(g)-380(o)-20.7(f)-385.7(t)-20.7(he)-391.6(h)-20.7(B)-.9(M)-20.7(S)-.6(C)-20.7(s)]TJ -8.9218 -1.1116 TD 0 Tc [(wa)18.8(s)-254.3(c)0(o)19.7(n)0(d)20.8(u)0(c)19.7(t)0(e)13.8(d)-254.5(to)-244.2(ob)20.8(se)19.9(rv)20.9(e)-255.7(t)0(h)15(e)-255.7(ce)18.5(ll)-244.4(ad)19.7(he)19.7(si)20.9(on)-244.1(on)-249.9(sc)19.9(af)19.7(fo)20.9(ld)20.7(.)-256.9(A)0(f)20(t)0(e)19.5(r)]TJ T* 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Uqu!~׶H9mo?_[l*м-ݏ34*+өDj?銦_-]~?wa#aDj??銻Ik\ߢ5gE]%o"boZ?l?7GYo1W|-v6F,ȷO-]#Py[LUk˜O UsKmn0MB.Jl;^{K)'jqgHW Όm+v/k/Պ@8;wJ{ש ]"Z''ԊmO5gE¬Kk\ߢ5gE[yoZ?l?7GYo1Vľ[#Qy[LU'Fs~kKk\ߢ5gE]%o"boZ?l?7GYo1VĞ[8iqo䎯 9Z9d$ۍWΣgL a7bgkȶ͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb endstream endobj 62 0 obj <>stream /GS1 gs BT /F2 1 Tf 8.9663 0 0 8.9663 69.7323 637.9085 Tm 0 0 0 rg 0 Tc 0 Tw [(ALP)-291.1(staining.)]TJ /F4 1 Tf 9.843 0 0 9.843 132.4346 637.9085 Tm [(Th)19.6(e)-203.8(l)0(e)13.8(v)0(e)19.7(l)-208.6(of)-198(AL)18.8(P)-202.5(a)0(c)12.8(t)0(i)20.6(v)0(i)20.7(t)0(y)-198.2(w)0(a)18.8(s)-208.2(ex)19.7(am)18.5(in)20.7(ed)-199.2(on)]TJ -7.3839 -1.1116 TD 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[(Fir)14(st-)14.1(Cha)11.8(in)-394(Syn)14.1(th)15(esi)12.9(s)-398.3(Kit)-384.6(\(Co)13(rni)13.9(ng)-393.9(Inc)12.9(or)15.1(por)14(ate)11.5(d\))-393.8(was)-385.3(use)13(d)]TJ T* [(for)-348.8(rev)12.8(ers)13(e)-359.4(tra)12.7(nsc)13(rip)13.9(tio)13.7(n.)-350.1(The)-351.2(tar)12.7(get)-350.2(PCR)-350.8(pri)19.6(mer)11.6(s)-358(for)-348.8(Run)12.9(t-)]TJ 0 -1.1174 TD [(rel)12.7(ate)11.5(d)-525.2(tra)12.7(nsc)13(rip)13.9(tio)19.5(n)-525.2(fac)11.7(tor)-516(\()]TJ /F5 1 Tf 11.9456 0 TD [(RU)13(NX2)]TJ /F4 1 Tf 3.0872 0 TD [(\),)-517.1(Ost)13.2(eoc)11.7(alc)11.5(in)-520.7(\()]TJ /F5 1 Tf 6.6467 0 TD (OCN)Tj /F4 1 Tf 2.0332 0 TD (\),)Tj -23.7127 -1.1116 TD [(SRY)12.2(-re)12.9(lat)12.6(ed)-400.8(HM)13.3(G)-405.1(box)-395(ge)13.9(ne)-400.8(9)-404.3(\()]TJ /F5 1 Tf 13.4892 0 TD [(SOX)17.7(9)]TJ /F4 1 Tf 2.3096 0 TD [(\),)-401.9(and)-390.4(Col)12.8(lag)12.7(en)-395(I)-404.2(a)0(1)]TJ -15.7989 -1.1116 TD (\()Tj /F5 1 Tf .3283 0 TD [(COL)12.2(IA1)]TJ /F4 1 Tf 3.3579 0 TD [(\))-260.2(wer)12(e)-261.4(syn)14.2(the)12.7(siz)12.9(ed)-256.8(by)-255.6(Inv)14(itr)13.8(oge)12.8(n)-260.3(\(Ta)11.7(ble)-252.3(1\).)]TJ /F10 1 Tf 17.521 0 TD (b)Tj /F4 1 Tf .5472 0 TD [(-Ac)12(tin)]TJ -21.7544 -1.1174 TD [(was)-287.4(use)13(d)-289.1(a)0(s)-291.1(a)0(n)-285.6(int)13.7(ern)12.8(al)-291.5(sta)18.6(nda)12.8(rd.)-282.1(The)-287.9(cyc)17.4(le)-291.5(thr)13.9(esh)13(old)-279.9(val)12.7(ues)]TJ 0 -1.1116 TD [(\(Ct)-257.9(val)12.7(ues)13(\))-260.3(wer)12(e)-267.2(use)13(d)-260.3(t)0(o)-261.5(cal)11.5(cul)12.7(ate)-253.4(the)-258(fol)13.9(d)-260.3(dif)13.9(fer)12.9(enc)11.7(es)-262.4(by)-255.6(the)]TJ T* [(Ct)-325.9(met)11.5(hod)-314.3(\(al)12.7(so)-324.5(kno)14(wn)-325.6(as)-325.7(the)-315.6(2)]TJ /F6 1 Tf 7.3823 0 0 6.5614 188.3905 362.7212 Tm (-)Tj /F12 1 Tf 7.4718 0 0 6.641 192.4157 362.7779 Tm (O)Tj .8195 0 TD (O)Tj /F4 1 Tf 7.3823 0 0 6.5614 206.6456 362.7212 Tm (Ct)Tj 9.843 0 0 9.843 216.8503 358.3558 Tm [(met)11.5(hod)14(\).)]TJ /F11 1 Tf 8.9663 0 0 8.9663 59.7543 336.3023 Tm [(Statistical)-336.1(analysis)]TJ /F4 1 Tf 9.843 0 0 9.843 69.7323 319.8613 Tm [(Data)-261.2(are)-264.8(represented)-264.3(as)-262.4(the)-258(mean)]TJ /F6 1 Tf 13.4547 0 TD ()Tj /F4 1 Tf .72 0 TD [(standard)-264.5(deviation)-260.3(and)]TJ -15.1883 -1.1116 TD [(were)-405(analyzed)-405.3(using)-402.9(SPSS)-401.2(17.0)-398.4(software.)-407(In)-399.6(terms)-399.5(of)-399.6(sta-)]TJ 0 -1.1174 TD [(tistical)-426.3(comparison,)-421.1(this)-419.2(study)-420.1(adopted)-424.9(the)]TJ /F5 1 Tf 18.1546 0 TD (t)Tj /F4 1 Tf .2822 0 TD [(-test)-421.4(and)-419.2(after)]TJ -18.4368 -1.1116 TD [(conrmatio)-8.9(n)-254.5(o)0(f)-261.4(homogeneity)-263.6(of)-261.4(variance,)-264.7(one-way)-264.3(ANOVA)]TJ T* [(as)-412.1(well)-404.1(as)-406.3(the)-407.8(LSD)-408.4(test)-408.8(were)-410.7(performed.)-408.5(The)-408.8(signicance)]TJ 0 -1.1174 TD [(level)-555.3(was)]TJ /F10 1 Tf 4.6078 0 TD (a)Tj /F6 1 Tf .72 0 TD (=)Tj /F4 1 Tf .72 0 TD [(0.05.)-551.6(*)]TJ /F5 1 Tf 3.0469 0 TD (p)Tj /F6 1 Tf .6681 0 TD (<)Tj /F4 1 Tf .72 0 TD [(0.05)-548.1(was)-552.3(considered)-550.1(to)-549.5(indicate)]TJ -10.4827 -1.1116 TD [(statistical)-338.5(signicance.)]TJ /F8 1 Tf 8.9663 0 0 8.9663 59.7543 232.044 Tm (Results)Tj /F11 1 Tf 0 -1.8336 TD [(Physical)-334.4(and)-335.7(chemical)-336.7(properties)-336.2(of)-330.4(printed)]TJ 0 -1.2203 TD [(3D)-336(scaffolds)]TJ /F2 1 Tf 1.1128 -1.8336 TD [(XPS)-227.7(analysis.)]TJ /F4 1 Tf 9.843 0 0 9.843 134.4188 188.2204 Tm [(T)15(h)0(e)-141.6(X)15.3(P)16.3(S)-150.7(sp)26.8(ec)24.3(t)16.1(r)0(u)26.6(m)-152.1(i)16.1(n)-150.8(Fi)26.6(gu)26.6(r)16.2(e)-152(1C)-141.5(e)15(x)16.2(hi)26.5(b)16.2(i)0(t)26.3(s)-150.7(th)26.5(e)]TJ -7.5855 -1.1116 TD [(ch)25.4(a)15(r)0(a)25.5(c)0(t)25.3(e)15(ri)26.5(st)26.7(ic)-176.3(e)15(l)0(e)25.3(m)15(en)25.4(ts)-174.9(of)-175(Ca)24.4(,)-187.7(P)16.3(,)-193.5(O)15.3(,)-187.7(a)15(nd)-175(C)-192.2(e)15(le)25.3(m)15(e)0(n)25.4(t)16.1(s)-191(i)16.1(n)-191.2(n)16.2(HA)-176.7(an)25.4(d)]TJ 0 -1.1174 TD [(DB)24.7(B)-186.4(c)15(ry)26.6(s)16.4(t)0(a)25.3(l)0(.)-171.7(T)15(he)-170.4(di)26.5(ff)26.7(r)16.2(a)0(c)24.3(t)0(i)26.3(o)16.2(n)-185.4(p)16.2(ea)24.3(ks)-169(we)24.6(re)-170.4(an)25.4(a)15(l)0(y)26.5(z)15(ed)-176.2(u)16.2(s)16.4(in)26.5(g)-185.4(C)15.2(AS)25.9(A)]TJ 0 -1.1116 TD [(XP)25.9(S)-386.8(s)0(o)26.8(f)0(t)26.5(w)15.3(ar)25.5(e)-388.2(a)0(n)25.4(d)-387(t)16.1(h)0(e)-377.7(C)15.2(a)15(/)0(P)-376.5(r)16.2(at)25.3(i)16.1(o)-392.8(c)15(a)15(l)0(c)25.3(u)16.2(la)25.3(te)25.3(d)16.2(.)-389.3(T)15(h)0(e)-377.7(r)16.2(e)15(s)0(u)26.8(l)0(t)-376.8(d)16.2(is)26.7(-)]TJ T* [(pl)26.5(ay)25.4(e)15(d)-156.6(th)26.5(a)15(t)-156.7(Ca)24.4(/)16.1(P)-156.4(ra)25.5(t)16.1(i)0(o)-146.3(i)16.1(s)-156.4(1)16.2(.)13.9(6)0(7)-146.2(f)16.2(or)-140.4(nH)25.7(A)-157.5(a)15(nd)-146.2(1)16.2(.)13.9(5)16.2(8)-156.6(fo)26.6(r)-156.6(D)15.3(BB)24.5(,)-158.9(b)16.2(o)16.2(t)0(h)-146.3(o)16.2(f)]TJ 0 -1.1174 TD [(wh)25.7(ic)25.3(h)-196.9(w)15.3(er)25.5(e)-198.1(c)15(on)26.6(si)26.7(st)26.7(e)15(n)0(t)-186.6(w)15.3(it)26.3(h)-196.9(t)0(h)26.5(o)16.2(se)-187.5(r)16.2(e)0(p)25.4(o)16.2(rt)26.5(ed)-187.7(p)16.2(r)0(e)25.5(v)16.2(io)26.5(u)16.2(s)0(l)26.7(y)0(.)]TJ 7.3823 0 0 6.5614 263.9054 137.7638 Tm -.0162 Tc [(22)7.7(26)]TJ 9.843 0 0 9.843 283.7479 133.3984 Tm 0 Tc [(Th)25.4(e)]TJ -22.7566 -1.1116 TD [(Ca)24.4(/)16.1(P)-277.4(ra)25.5(t)16.1(i)0(o)-261.5(i)0(n)-267.3(D)15.3(B)15.2(B)-278.6(i)16.1(s)-277.4(l)16.1(o)0(w)25.7(e)0(r)-262.5(t)0(h)26.5(a)15(n)-277.6(i)0(n)-261.5(n)0(H)25.7(A)-278.4(p)16.2(o)16.2(s)0(s)27(i)0(b)26.5(l)0(y)-261.5(b)0(e)25.4(c)15(au)25.4(se)-262.4(th)26.5(e)]TJ T* (Ca)Tj 7.3823 0 0 6.5614 70.4693 109.8708 Tm -.0162 Tc (10)Tj 9.843 0 0 9.843 79.37 111.5149 Tm [(\(P)-16.2(O)]TJ 7.3823 0 0 6.5614 94.7338 109.8708 Tm 0 Tc (4)Tj 9.843 0 0 9.843 98.3055 111.5149 Tm (\))Tj 7.3823 0 0 6.5614 101.4236 109.8708 Tm (6)Tj 9.843 0 0 9.843 104.9952 111.5149 Tm [(\(O)25.8(H)15.3(\))]TJ 7.3823 0 0 6.5614 126.9354 109.8708 Tm (2)Tj 9.843 0 0 9.843 132.2645 111.5149 Tm [(in)-152.1(th)26.5(e)-169.3(c)15(a)15(l)0(f)-157.8(b)16.2(o)16.2(n)0(e)-153.1(w)0(a)24.6(s)-167.9(p)16.2(a)0(r)25.5(t)0(i)26.3(a)15(ll)26.3(y)-168.1(c)15(on)26.6(v)16.2(e)0(r)25.5(t)0(e)25.3(d)-168.1(t)16.1(o)]TJ /F10 1 Tf 16.0581 0 TD (b)Tj /F4 1 Tf .5357 0 TD (-)Tj -23.9604 -1.1174 TD (Ca)Tj 7.3823 0 0 6.5614 70.4693 98.8724 Tm (3)Tj 9.843 0 0 9.843 77.2724 100.5165 Tm [(\(P)26.8(O)]TJ 7.3823 0 0 6.5614 92.6362 98.8724 Tm (4)Tj 9.843 0 0 9.843 96.2078 100.5165 Tm (\))Tj 7.3823 0 0 6.5614 99.3826 98.8724 Tm (2)Tj 9.843 0 0 9.843 106.1858 100.5165 Tm [(d)16.2(u)0(r)26.6(i)16.1(ng)-301.7(th)26.5(e)-319(c)15(al)25.3(c)15(i)0(n)26.5(a)15(ti)26.3(on)-301.7(pr)26.6(oc)25.4(e)15(s)0(s)27(,)-320.2(r)16.2(e)15(su)26.8(lt)26.3(i)16.1(n)0(g)-307.5(t)16.1(h)16.2(e)-319(C)15.2(a)0(/)25.3(P)]TJ -4.7172 -1.1116 TD [(ra)25.5(ti)26.3(o)-248.8(i)16.1(n)-243(D)0(B)24.7(B)-249.8(l)16.1(e)15(ss)-238(t)16.1(h)16.2(an)-233.7(1.)24.3(6)16.2(7)-248.8(i)16.1(n)-243(hy)26.6(dr)26.6(ox)26.6(y)16.2(a)0(p)25.4(a)15(ti)26.3(te)25.3(.)]TJ 7.3823 0 0 6.5614 236.8062 93.9401 Tm -.0171 Tc (9,10)Tj /F2 1 Tf 8.9663 0 0 8.9663 69.7323 67.5212 Tm 0 Tc [(SEM)-357.8(of)-355.7(nHA)-359.2(and)-361(DBB.)]TJ /F4 1 Tf 9.843 0 0 9.843 172.5165 67.5212 Tm [(The)-357(surface)-356.5(morph)-6.7(ology)-351.2(of)-353.5(the)]TJ -11.4561 -1.1116 TD [(two)-384.4(HA)-389.8(under)-381(SEM)-385.4(is)-388(shown)-380.6(in)-388.2(Figure)-382.1(1D)-383.2(and)-384.6(E.)-385.9(A)-382.1(few)]TJ 25.5097 58.9967 TD [(agglomeration)-445.6(particles)-445.5(were)-439.5(observed)-442.9(in)-440.1(nHA)-442.8(\(Fig.)-439.7(1D\).)]TJ 0 -1.1116 TD [(While)-406.6(the)-407.8(surface)-408.4(images)-407.5(of)-405.3(DBB)-403.7(\(Fig.)-405.2(1E\))-407.6(exhibited)-410(the)]TJ T* [(evenly)-539(distributed)-543.5(micropores,)-542(and)-540.1(few)-541(interconnecttions)]TJ 0 -1.1174 TD [(between)-438.4(the)-436.6(pores,)-441.9(which)-433.9(is)-439.9(similar)-436.5(with)-437.5(the)-436.6(nature)-441(bone)]TJ 0 -1.1116 TD [(trabecula.)-484.8(The)-483.7(micropore)-480.1(diameter)-486.1(from)-483.6(DBB)-478.5(was)-483.2(about)]TJ T* (1)Tj /F10 1 Tf .6681 0 TD (m)Tj /F4 1 Tf .5472 0 TD [(m,)-472.3(which)-468.4(were)-474.1(not)-470(presented)-468.3(in)-468.9(nHA.)-469.3(Both)-472.1(nHA)-471.6(and)]TJ -1.2153 -1.1174 TD [(DBB)-346.1(crystals)-350.8(were)-353.2(spherical)-353.3(and)-344.3(have)-352.3(similar)-350.1(diameters)-354.5(in)]TJ 0 -1.1116 TD [(the)-332.9(images)-338.4(inserted)-333.7(to)-336.4(\(Fig.)-330.3(1D,)-334.8(E\).)]TJ /F2 1 Tf 8.9663 0 0 8.9663 320.8251 538.5825 Tm [(The)-457.1(construction)-463.1(of)-463.2(3D)-462.5(printed)-462.9(scaffold.)]TJ /F4 1 Tf 9.843 0 0 9.843 494.5321 538.5825 Tm [(The)-454.9(scaffolds)]TJ -18.6614 -1.1174 TD [(printed)-314.4(were)-324.4(fabricated)-321.1(successfully)-321.6(as)-314.2(shown)-317.2(in)-319.1(Figure)-318.7(1A)]TJ 0 -1.1116 TD [(and)-315.5(B.)-322.3(After)-318.6(printing,)-317.9(a)-319(grid-like)-316.7(structure)-323.3(was)-321.9(constructed.)]TJ T* [(The)-512.5(diameter)-520.6(of)-514.8(up)-509.1(and)-517.1(down)-512.1(conne)-7.8(cted)-513.7(square)-515.6(hole)-512.5(is)]TJ T* (600)Tj /F10 1 Tf 1.6646 0 TD (m)Tj /F4 1 Tf .5529 0 TD [(m.)-380.1(The)-385.8(DBB)-380.6(scaffolds)-386.3(were)-387.7(more)-381.1(feasible)-386.7(to)-376.7(bottom)]TJ -2.2175 -1.1174 TD [(up)-330.5(a)-336.3(higher)-336.2(construction)-332.5(during)-335(the)-338.7(printing)-331.7(\(Fig.)-336.1(1B\).)]TJ /F2 1 Tf 8.9663 0 0 8.9663 320.8251 461.7636 Tm [(SEM)-300.9(of)-305.1(3D)-304.4(printed)-311.2(scaffold.)]TJ /F4 1 Tf 9.843 0 0 9.843 440.5605 461.7636 Tm [(The)-310.9(surface)-310.4(morphology)-311.9(of)]TJ -13.1782 -1.1116 TD [(the)-315.6(scaffold)-322(after)-318.9(being)-317.8(lyophilized)-320.2(under)-317.7(SEM)-322(is)-313.2(shown)-323(in)]TJ 0 -1.1174 TD [(Figure)-272.7(1FH.)-267.7(All)-269.4(the)-269.5(scaffolds)-276.9(had)-269.4(porous)-271.3(structure,)-274.9(and)-269.4(the)]TJ 0 -1.1116 TD [(pore)-259(size)-265.8(was)-264.3(ranged)-262.3(from)-264.8(100)-262.5(to)-261.5(300)]TJ /F10 1 Tf 15.3496 0 TD (m)Tj /F4 1 Tf .5529 0 TD [(m.)-259.2(The)-264.9(CoL)-264.7(scaffold)]TJ -15.9025 -1.1116 TD [(\(Fig.)-364.9(1F\))-366(was)-368(presented)-370.4(an)-366.2(irregular)-369.5(lamellar)-371(structure)-369.3(and)]TJ 0 -1.1174 TD [(the)-430.8(heterogeneous)-433.6(pore)-431.8(distribution)-434.1(with)-431.7(pore)-426(size)-432.9(ranged)]TJ 0 -1.1116 TD [(from)-443.3(60)-439.9(to)-445.8(300)]TJ /F10 1 Tf 6.7216 0 TD (m)Tj /F4 1 Tf .5529 0 TD [(m,)-443.5(and)-442.2(a)-445.8(little)-446.1(deformation)-442.1(of)-445.7(the)-442.3(pores)]TJ -7.2745 -1.1116 TD [(could)-260.2(be)-262.6(seen)-265.7(on)-261.4(collagen)-266(scaffold.)-262.1(The)-264.8(surface)-264.4(morphology)]TJ 0 -1.1174 TD [(of)-347.8(nHA/CoL)-351.6(and)-350(DBB/CoL)-352.8(scaffolds)-351.7(\(Fig.)-353.4(1G,)-346.3(H\))-348.6(showed)]TJ 0 -1.1116 TD [(the)-263.8(regular)-269.3(porous)-271.3(structure)-265.7(distributed.)-270.4(The)-270.6(images)-269.3(inserted)]TJ T* [(to)-376.7(\(Fig.)-376.4(1G,)-375.1(H\))-383.2(were)-376.2(magnied)-381(to)-376.7(see)-379.8(the)-379(details)-378.8(of)-376.5(nHA/)]TJ 0 -1.1174 TD [(CoL)-241.7(and)-246.4(DBB/CoL)-249.2(scaffolds.)-245.7(nHA)-247(evenly)-245.2(distributed)-244(on)-244.1(the)]TJ 0 -1.1116 TD [(collagen)-398.5(surface)-402.6(due)-396.1(to)-399.7(the)-396.3(small)-399.6(particle,)-402(while)-399.5(the)-396.3(DBB)]TJ T* [(particle)-243(could)-237.2(be)-245.3(clearly)-241.8(seen)-242.7(in)-238.5(DBB/CoL)-243.4(scaffolds)-242.3(surface.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 320.8251 297.2409 Tm [(FTIR)-302.2(analysis.)]TJ /F4 1 Tf 9.843 0 0 9.843 388.1763 297.2409 Tm [(Figure)-307.2(2A)-308.3(and)-309.7(B)-301.6(show)-310.3(the)-304.1(FTIR)-305.9(spectr)-8.8(a)]TJ -7.8562 -1.1116 TD [(of)-359.3(the)-361.7(material)-365.2(and)-361.6(scaffolds.)-360.9(In)-365(nHA,)-359.8(the)-361.7(peaks)-364.7(at)-360.6(3568.2)]TJ T* [(and)-373.1(3440.5)-164.4(cm)]TJ /F6 1 Tf 7.3823 0 0 6.5614 369.581 279.7228 Tm (-)Tj /F4 1 Tf .5529 0 TD (1)Tj 9.843 0 0 9.843 381.033 275.3574 Tm [(belong)-370.8(to)-370.9(the)-373.2(OH-)-379.4(stretching)-370.6(vibration)-380.1(of)]TJ -7.1305 -1.1116 TD (hydroxyl.)Tj 7.3823 0 0 6.5614 348.8881 268.781 Tm (27)Tj 9.843 0 0 9.843 360.4534 264.4157 Tm [(Furthermore,)-427.9(the)-425.1(major)-428.5(bonds)-425.8(associated)-430.2(with)]TJ -5.0397 -1.1174 TD [(PO43-\(1037,)-585.3(603,)-582.7(and)-586.2(567)-170.3(cm)]TJ /F6 1 Tf 7.3823 0 0 6.5614 439.1432 257.7826 Tm (-)Tj /F4 1 Tf .5529 0 TD (1)Tj 9.843 0 0 9.843 446.9101 253.4173 Tm [(\))-588.6(appeared)-589.4(in)-584(nHA)-586.8(and)]TJ -13.8233 -1.1116 TD (DBB.)Tj 7.3823 0 0 6.5614 333.5243 246.8409 Tm (28)Tj 9.843 0 0 9.843 343.6156 242.4755 Tm [(The)-276.4(peak)-283.2(at)-274.2(33303428.6)-170(cm)]TJ /F6 1 Tf 7.3823 0 0 6.5614 458.4188 246.8409 Tm (-)Tj /F4 1 Tf .5606 0 TD (1)Tj 9.843 0 0 9.843 468.9636 242.4755 Tm [(belongs)-279.5(to)-273(the)]TJ /F5 1 Tf 5.9555 0 TD (N)Tj /F6 1 Tf .8352 0 TD (=)Tj /F4 1 Tf .7142 0 TD (H)Tj -23.5687 -1.1116 TD [(stretching)-503.1(of)-497.5(Amide)-498.5(A.)-500.7(The)-501(peak)-502.1(at)-498.8(1655)-165.7(cm)]TJ /F6 1 Tf 7.3823 0 0 6.5614 503.4329 235.8992 Tm (-)Tj /F4 1 Tf .5529 0 TD (1)Tj 9.843 0 0 9.843 516.1321 231.5338 Tm [(is)-497.5(C)]TJ /F6 1 Tf 1.9986 0 TD (=)Tj /F4 1 Tf .7142 0 TD (O)Tj -23.5687 -1.1174 TD [(absorption)-248.4(of)-244.1(Amide)-250.8(I,)-246.4(1550)-165.7(cm)]TJ /F6 1 Tf 7.3823 0 0 6.5614 436.4219 224.9574 Tm (-)Tj /F4 1 Tf .5529 0 TD (1)Tj 9.843 0 0 9.843 446.6266 220.5354 Tm [(is)-244(C-N)-252.8(stretching)-249.6(and)-246.4(C-H)]TJ -13.7945 -1.1116 TD 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[(distributed)-330.4(in)-324.9(the)-327.1(cytoplasm)-330.3(and)-327(enriched)-329.2(at)-331.8(the)-327.1(end)-327(of)-324.7(cell)]TJ T* [(pseudopodia)-8.6(,)-400.8(which)-410.9(showed)-405.9(enhanced)-410.9(uorescence)-415(signal.)]TJ T* [(The)-345.5(cells)-347.7(on)-342(the)-344.4(nHA/CoL)-345.9(and)-338.5(DBB/CoL)-347.1(scaffolds)-346(altered)]TJ 0 -1.1174 TD [(to)-399.7(a)-393.9(plate-like)-400.9(morphology)-392.5(in)-394(contrast)-397.1(to)-399.7(the)-390.5(spindle)-400.6(mor-)]TJ 0 -1.1116 TD [(phology)-664.5(observed)-661.8(on)-658.8(CoL)-662.1(scaffolds.)-666.2(The)-662.3(white)-664.4(boxes)]TJ T* [(\(Fig.)-347.6(4E,)-340.9(F\))-341.8(represented)-350.7(nHA)-339.1(and)-344.3(the)-344.4(degradation)-344.1(particles)]TJ 0 -1.1174 TD [(of)-474.5(DBB,)-470.5(respectively.)-476.5(The)-472.2(cells)-474.4(can)-472.2(be)-475.7(seen)-473.1(well)-473.2(grown)]TJ 0 -1.1116 TD [(around)-336(the)-338.7(HA)-332.2(parties)-338.4(in)-330.6(scaffolds.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 323.0361 67.5212 Tm [(Proliferation)-382.6(of)-374.7(hBMSCs)-377.2(in)-374.4(3D)-380.3(scaffolds.)]TJ /F4 1 Tf 9.843 0 0 9.843 496.3463 67.5212 Tm [(The)-305.2(cells)-301.6(pro-)]TJ -18.6211 -1.1116 TD [(liferation)-169.3(in)-157.8(different)-167.9(scaffolds)-161.7(was)-166.4(evaluated)-164.6(by)-163.5(CCK-8)-163.9(assay)]TJ ET q 489.531 0 0 393.732 62.532 338.513 cm /Im2 Do Q BT /F7 1 Tf 9.845 0 0 9.845 62.022 321.7322 Tm [(FIG.)-348.3(2.)-1000.6(\(A,)-345.9(B\))]TJ /F4 1 Tf 7.1233 0 TD [(are)-345.1(the)-349.8(FTIR)-345.7(patterns)-350.2(of)-347.5(nHA,)-347.7(nHA/CoL,)-348.3(DBB,)-348.9(and)-343.9(DBB/CoL;)]TJ /F5 1 Tf 26.6793 0 TD [(Dashed)-347.5(lines)]TJ /F4 1 Tf 5.6376 0 TD [(indicate)-351.7(similar)-343.6(symbolic)]TJ -39.4403 -1.0077 TD [(peaks)-387.2(of)-382(the)-390.1(two)-384(groups:)-381.2(1037)-171(cm)]TJ /F6 1 Tf 7.3837 0 0 6.5625 201.4299 316.1763 Tm (-)Tj /F4 1 Tf .5528 0 TD (1)Tj 9.845 0 0 9.845 209.1968 311.8109 Tm (:)Tj /F10 1 Tf .668 0 TD (n)Tj /F4 1 Tf .501 0 TD [(3)-381.1(vibration)-390.7(of)-382(PO4)]TJ 7.3837 0 0 6.5625 298.2613 316.1763 Tm (3)Tj /F6 1 Tf .5068 0 TD (-)Tj /F4 1 Tf 9.845 0 0 9.845 306.085 311.8109 Tm [(;606)-384.2(and)-384.2(562)-164.2(cm)]TJ /F6 1 Tf 7.3837 0 0 6.5625 373.9464 316.1763 Tm (-)Tj /F4 1 Tf .5528 0 TD (1)Tj 9.845 0 0 9.845 381.7133 311.8109 Tm (:)Tj /F10 1 Tf .2822 0 TD (n)Tj /F4 1 Tf .501 0 TD [(4)-381.1(vibration)-384.9(of)-387.8(PO4)]TJ 7.3837 0 0 6.5625 466.9794 316.1763 Tm (3)Tj /F6 1 Tf .5068 0 TD (-)Tj /F4 1 Tf 9.845 0 0 9.845 474.803 311.8109 Tm [(;)-381.2(1655)-165.2(cm)]TJ /F6 1 Tf 7.3837 0 0 6.5625 514.7715 316.1763 Tm (-)Tj /F4 1 Tf .5528 0 TD (1)Tj 9.845 0 0 9.845 522.5385 311.8109 Tm (:Amide)Tj -46.7767 -1.0135 TD [(I;1550)-167.4(cm)]TJ /F6 1 Tf 7.3837 0 0 6.5625 101.4803 306.1984 Tm (-)Tj /F4 1 Tf .5605 0 TD (1)Tj 9.845 0 0 9.845 109.3039 301.833 Tm [(:)-237.3(Amide)-244.4(II;)-239.3(1237)-165.2(cm)]TJ /F6 1 Tf 7.3837 0 0 6.5625 188.7874 306.1984 Tm (-)Tj /F4 1 Tf .5528 0 TD (1)Tj 9.845 0 0 9.845 196.5543 301.833 Tm [(:Amide)-239.8(III.)]TJ /F7 1 Tf 4.7393 0 TD [(\(C,)-242.3(D\))]TJ /F4 1 Tf 2.8332 0 TD [(are)-241.4(X-ray)-243(diffraction)-244.1(patterns)-240.8(of)-238.1(nHA,)-244.1(nHA/CoL,)-238.9(DBB,)-245.3(and)-240.2(DBB/CoL;)]TJ -21.2375 -1.0135 TD (The)Tj /F5 1 Tf 1.8543 0 TD [(black)-289.6(dots)]TJ /F4 1 Tf 4.4168 0 TD (in)Tj /F7 1 Tf 1.0711 0 TD (\(C)Tj /F4 1 Tf 1.0538 0 TD (,)Tj /F7 1 Tf .5355 0 TD (D\))Tj /F4 1 Tf 1.3417 0 TD [(represent)-294.6(the)-286.5(Ca)]TJ 7.3837 0 0 6.5625 228.1322 290.2109 Tm (10)Tj 9.845 0 0 9.845 235.5023 291.855 Tm (\(PO)Tj 7.3837 0 0 6.5625 251.3196 290.2109 Tm (4)Tj 9.845 0 0 9.845 255.0047 291.855 Tm (\))Tj 7.3837 0 0 6.5625 258.2928 290.2109 Tm (6)Tj 9.845 0 0 9.845 261.9779 291.855 Tm (\(OH\))Tj 7.3837 0 0 6.5625 282.6708 290.2109 Tm (2)Tj 9.845 0 0 9.845 289.2472 291.855 Tm [(phases;)-291.3(The)]TJ /F5 1 Tf 5.0848 0 TD [(black)-289.6(pentagram)]TJ /F4 1 Tf 7.0888 0 TD (in)Tj /F7 1 Tf 1.0711 0 TD (\(D\))Tj /F4 1 Tf 1.67 0 TD [(represents)-295.4(the)]TJ /F10 1 Tf 5.868 0 TD (b)Tj /F4 1 Tf .5528 0 TD [(-TCP)-293.9(phases.)]TJ -44.4157 -1.0077 TD [(FTIR,)-337.6(Fourier)-330.6(transform)-339.5(infrared)-332.9(spectroscopy.)-341.1(Color)-334.4(images)-331.9(are)-339.3(available)-336.6(online.)]TJ /F8 1 Tf 8.9663 0 0 8.9663 62.022 749.6502 Tm (1266)Tj 50.2102 0 TD [(LI)-331.6(ET)-338.7(AL.)]TJ ET endstream endobj 71 0 obj <>stream Adobed       5  s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjzm!1AQa"q2#BRbr3$4CS%cs5DT &6E'dtU7()󄔤euFVfvGWgw8HXhx9IYiy*:JZjz?g<ƾ\򞧬=KXOf!Oa4;]U]'BWsa`OcQjs~Ni[,7WkQq BI_*3|$oɿ'y{QkQKKk;oW?KOy}+G ԖMp\V{A-?#w9RZF7s_7qZ=o瞿oo{'Iiq=hO7yÞzkw?%n<S{>?KOy}+G ԖMp\V{A-?#w9RZF7s_7qZ=o瞿oo{'Iiq=hO7yÞzkw?%n<S{>?KOy}+G ԖMp\V{A-?#w9RZF7s_7qZ=o瞿oo{'Iiq=hO7yÞzkw?%n<S{>?KOy}+G ԖMp\V{A-?#w9RZF7s_7qZ=o瞿oo{'Iiq=hO7yÞzkw?%n<S{>?KOy}+G ԖMp\V{A-?#w9RZF7s_7qZ=o瞿oo{'Iiq=hO7yÞzkw?%n<S{>?KOy}+G ԖMp\V{A-?#w9RZF7s_7qZ=o瞿oo{'Iiq=hO7yÞzkw?%n<S{>?KOy}+G ԖMp\V{A-?#wS5 WWvחvps[y5bBĬJfѮnm,loVդi6gP0 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Bg2u%&eR\?j%+ihWGn1ARca9j~N5ӟ OFM?M6s0/ǵp T榻*Z5ЂDeJR]K۸ף.lƭ!1ASCҸxWW%ѤUኳpF HyQSZiwq(:ч.A@]%o51YyHX*RDnD2~KnnӉ}a?k:>0hGwn(yS}"Ȓܫqg'l>yW}?jp2 ~?vB5O\/wNtJu[a 'U%?K.'ő>stream /GS1 gs BT /F4 1 Tf 9.843 0 0 9.843 59.7543 470.4377 Tm 0 0 0 rg 0 Tc 0 Tw [(at)-251.2(1,)-246.4(3,)-252.2(5,)-246.4(and)-252.1(7)-248.8(days)-247.3(after)-249.8(seeding)-251.9(as)-250.8(shown)-248.1(in)-250(Figure)-249.6(5.)-252.2(The)]TJ 0 -1.1116 TD [(OD)-418.6(values)-412(of)-416.9(all)-413.7(groups)-415.3(increased)-417.6(over)-414.5(time.)-413.7(The)-414.6(growth)]TJ T* [(curves)-360(indicated)-353.6(that)-357.2(the)-350.2(overall)-355.8(growth)-353.2(tendencies)-361.3(in)-353.7(both)]TJ 0 -1.1174 TD [(groups)-231(were)-226.4(similar)-229.1(during)-225.6(the)-223.5(culture)-229.1(process.)-226.2(However,)-228.3(the)]TJ 0 -1.1116 TD [(cell)-260.4(proliferation)-259(was)-252.8(higher)-261.3(in)-250(CoL)-259(group)-253.1(than)-259.1(in)-250(nHA/CoL)]TJ T* [(and)-263.7(DBB/CoL)-260.7(group)-258.9(at)-256.9(5th)-262.6(and)-257.9(7th)-256.9(day)-257.9(\()]TJ /F5 1 Tf 16.6513 0 TD (p)Tj /F6 1 Tf .6681 0 TD (<)Tj /F4 1 Tf .72 0 TD [(0.05\).)-258.9(The)-259.1(cells)]TJ -18.0394 -1.1174 TD [(proliferation)-247.5(on)-244.1(nHA/CoL)-248(and)-240.6(DBB/CoL)-249.2(was)-241.3(consistent)-243.7(and)]TJ 0 -1.1116 TD [(had)-263.7(no)-261.4(signicant)-261(difference)-269.1(\()]TJ /F5 1 Tf 12.2394 0 TD (p)Tj /F6 1 Tf .6681 0 TD (>)Tj /F4 1 Tf .72 0 TD (0.05\).)Tj /F2 1 Tf 8.9663 0 0 8.9663 69.7323 363.5716 Tm [(ALP)-436.5(staining.)]TJ /F4 1 Tf 9.843 0 0 9.843 133.7385 363.5716 Tm [(The)-426.1(osteogenic)-435(potential)-427.5(of)-422.6(hBMSCs)-429.7(in)]TJ -7.5164 -1.1174 TD [(different)-294.6(scaffolds)-294.1(was)-287.4(evaluated)-297.1(by)-290.2(ALP)-287.4(staining)-292.4(as)-291.2(shown)]TJ 0 -1.1116 TD [(in)-273(Figure)-272.7(6.)-269.5(After)-272.6(7)-266(days)-270.3(of)-272.9(culturing,)-274.1(the)-269.5(result)-272.7(showed)-273.4(that)]TJ T* [(cells)-336.2(in)-336.4(PM)-331.2(did)-337.5(not)-331.7(display)-331.5(signicantly)-338.3(staining)-338.5(\(Fig.)-330.3(6A)]TJ T* [(C\),)-663.2(while)-664.4(cells)-664.5(in)-658.9(OM)-666.3(showed)-665.1(positive)-661(ALP)-661.8(staining)]TJ 25.5097 41.1877 TD [(\(Fig.)-347.6(6DF\).)-343.7(The)-351.2(brown)-346.2(spindle)-348.8(morphology)-346.4(in)-347.9(red)-344.3(dashed)]TJ 0 -1.1174 TD [(box)-239.4(\(Fig.)-238.2(6DF\))-236.6(represented)-247(the)-235(expression)-243.4(of)-238.3(ALP)-241.4(in)-238.5(MSCs)]TJ 0 -1.1116 TD [(cells,)-356.9(respectivel)-11.2(y.)-355.9(Cells)-359.1(in)-359.4(PM)-360(showed)-359.8(more)-358.1(obvious)-359(pro-)]TJ T* [(liferation)-376.6(effect,)-382.2(and)-378.8(the)-373.2(numbers)-378.5(of)-376.5(hBMSCs)-377.9(cells)-382.3(in)-376.7(PM)]TJ T* [(were)-335.9(greater)-333.8(than)-334(that)-339.9(in)-330.6(OM)-332.3(group.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 320.8251 656.6738 Tm .0182 Tc [(Ost)-4.3(e)-5.2(oge)-5.2(ne)-5.2(si)-4.1(s-rel)-4.1(a)1.1(t)-4.3(e)-5.2(d)-839.8(gen)-5.2(e)-839.8(e)1.1(x)-6.6(p)1.1(res)-6.6(s)-.3(i)-4.1(o)1.1(n)-839.8(i)-4.1(n)-839.8(h)1.1(B)-5(M)-2.4(SCs.)]TJ /F4 1 Tf 9.843 0 0 9.843 310.8472 645.7321 Tm 0 Tc [(Osteogenesis-related)-398.4(gene)-392.6(expression)-393.2(could)-392.7(reect)-395.9(the)-390.5(dif-)]TJ T* [(ferentiation)-368.5(of)-267.1(hB)19.8(MS)20.1(Cs)-273.8(in)20.7(to)-267.3(os)15.3(te)19.5(ob)20.8(la)19.5(st)20.9(s.)-269.3(Af)14.2(te)19.5(r)-277.5(t)0(h)20.7(e)-278.7(cu)13.9(lt)20.6(ur)20.9(e)-278.7(o)0(f)]TJ 0 -1.1174 TD [(hB)19.8(MS)20.1(Cs)-285.3(on)-284.4(th)20.7(re)19.7(e)-296(g)0(r)20.9(o)0(u)15.1(p)0(s)-284.2(o)0(f)-284.4(s)0(c)19.9(a)0(f)19.7(f)0(o)20.9(l)0(d)15(s)-294.6(fo)20.9(r)-294.8(7)-294.8(d)0(a)19.7(y)0(s)21(,)-291.4(th)15(e)-290.3(m)0(R)18.6(N)0(A)]TJ 0 -1.1116 TD [(le)19.6(ve)19.7(ls)-278.6(of)-284.4(os)21(te)19.6(og)20.8(en)13.9(es)19.9(is)20.9(-r)20.9(el)13.8(at)19.5(ed)-279.8(ge)19.7(ne)19.7(s)-288.9(o)0(f)]TJ /F5 1 Tf 15.58 0 TD [(OC)18.9(N,)-282(RU)18.8(NX)18.6(2,)-281(SO)20(X9)19.6(,)]TJ /F4 1 Tf -15.58 -1.1116 TD [(an)19.7(d)]TJ /F5 1 Tf 1.7106 0 TD [(CO)18.9(LI)21(A1)]TJ /F4 1 Tf 3.6171 0 TD [(we)18.8(re)-268.3(de)13.9(te)19.5(ct)19.5(ed)-274.1(us)21(in)20.7(g)-283.3(r)0(e)19.7(a)0(l)19.5(-)0(t)20.7(i)0(m)19.5(e)-284.5(po)20.8(ly)20.7(me)18.4(ra)19.7(se)-273.9(ch)19.7(ai)19.5(n)]TJ -5.3277 -1.1174 TD [(re)19.7(ac)18.5(ti)20.6(on)-255.6(\(R)19.8(T-)19.7(PC)14.2(R\))19.8(,)-262.6(a)0(n)19.7(d)-266(th)20.7(e)-267.2(r)0(e)19.7(s)0(u)21(l)0(t)14.8(s)-265.8(ar)19.7(e)-261.5(s)0(h)15.3(o)0(w)20(n)-266(in)-255.8(Fi)20.9(gu)20.8(re)-256.8(7.)-252.2(As)]TJ 0 -1.1116 TD [(th)20.7(e)-157.8(r)0(e)19.7(s)0(u)21(l)0(t)20.6(s)-156.4(di)14.9(sp)21(la)19.6(y,)-148.5(th)20.7(e)-157.8(o)0(s)21(t)0(e)19.5(o)0(g)20.8(e)0(n)19.7(e)0(s)14.1(i)0(s)20.9(-)0(r)20.9(e)0(l)19.5(a)0(t)19.5(e)0(d)-147.4(g)0(e)19.7(n)0(e)13.9(s)-156.4(ex)19.7(pr)20.9(es)19.9(si)20.9(on)-146.2(in)]TJ T* [(OM)-188.3(gr)20.9(ou)20.8(p)-196.9(a)0(r)19.7(e)-198.1(si)20.9(gn)20.8(i)20.9(ca)18.5(nt)14.9(ly)-180.9(hi)14.9(gh)20.8(er)-187.6(th)20.7(an)-187.7(th)20.7(e)-198.1(p)0(a)19.7(r)0(a)19.7(l)0(l)20.6(e)0(l)-187.8(g)0(r)20.9(o)0(u)20.8(p)-196.9(de)19.7(al)19.5(t)]TJ 0 -1.1174 TD [(wi)19.8(th)-353.7(PM)-348.5(\()]TJ /F5 1 Tf 4.3889 0 TD (p)Tj /F6 1 Tf .6566 0 TD (<)Tj /F4 1 Tf .7084 0 TD [(0.)18.5(01)20.8(\).)-355.8(In)-347.7(OM)-355.3(gr)20.9(ou)20.8(p,)-355.9(th)20.7(e)-359.4(e)0(x)19.7(p)0(r)15.1(e)0(s)19.9(s)0(i)20.9(o)0(n)-353.5(o)0(f)]TJ /F5 1 Tf 15.9947 0 TD [(SO)20(X9)19.6(,)]TJ -21.7486 -1.1116 TD [(OC)18.9(N,)]TJ /F4 1 Tf 2.6552 0 TD [(an)19.7(d)]TJ /F5 1 Tf 1.8143 0 TD [(CO)18.9(LI)21(A1)]TJ /F4 1 Tf 3.7208 0 TD [(ge)19.7(ne)-377.7(in)-370.9(hB)19.8(MS)20.1(Cs)-377.4(cu)19.7(lt)14.8(ur)20.9(ed)-372(in)-376.7(nH)20(A/)19.8(Co)14(L)]TJ -8.1903 -1.1116 TD [(sc)19.9(af)19.7(fo)20.9(ld)15(s)-432.9(i)0(s)-428.3(s)0(i)20.9(g)0(n)20.8(i)0()15.1(c)0(a)18.5(n)0(t)20.7(l)0(y)-428.5(h)0(i)20.7(g)0(h)20.8(e)0(r)-429.5(t)0(h)20.7(a)0(n)-423.8(t)0(h)15(a)0(t)-424(i)0(n)-428.5(C)0(o)19.8(L)-434.3(sc)14.1(af)19.7(fo)20.9(ld)20.7(s)]TJ 0 -1.1174 TD (\()Tj /F5 1 Tf .4896 0 TD (p)Tj /F6 1 Tf .6566 0 TD (<)Tj /F4 1 Tf .7084 0 TD [(0.)18.5(05)20.8(\).)-338.6(Th)19.6(e)]TJ /F5 1 Tf 4.5214 0 TD [(RU)18.8(NX)18.6(2)]TJ /F4 1 Tf 3.427 0 TD [(ex)19.7(pr)20.9(es)19.9(si)15.1(on)-336.3(in)-336.4(nH)20(A/)14.1(Co)19.8(L)-347.9(s)0(c)19.9(a)0(f)19.7(f)0(o)15.1(l)0(d)20.7(s)-346.5(is)]TJ -9.803 -1.1116 TD [(lo)20.7(we)18.8(r)-185.4(t)0(h)20.7(a)0(n)-170.4(t)0(h)20.7(a)0(t)-176.3(i)0(n)-169.4(C)0(o)19.8(L)-186.6(an)19.7(d)-179.6(D)0(B)18.9(B)0(/)19.7(C)0(o)14(L)-180.8(sc)19.9(af)19.7(fo)15.1(ld)20.7(s)-179.4(\()]TJ /F5 1 Tf 18.9149 0 TD (p)Tj /F6 1 Tf .6566 0 TD (<)Tj /F4 1 Tf .7084 0 TD [(0.)18.5(05)20.8(\).)-171.5(Th)13.9(e)]TJ -20.2799 -1.1116 TD [(ex)19.7(pr)20.9(es)19.9(si)15.1(on)-313.2(of)]TJ /F5 1 Tf 5.6618 0 TD [(OC)18.9(N)]TJ /F4 1 Tf 2.3557 0 TD [(an)19.7(d)]TJ /F5 1 Tf 1.7509 0 TD [(CO)18.9(LI)21(A1)]TJ /F4 1 Tf 3.6574 0 TD [(in)-313.3(DB)18.9(B/)19.7(Co)14(L)-319.1(s)0(c)14.1(a)0(f)19.7(f)0(o)20.9(l)0(d)20.7(s)-323.5(wa)18.8(s)]TJ -13.4259 -1.1116 TD [(hi)20.7(gh)20.8(er)-245.2(th)15(an)-245.3(th)20.7(at)-245.4(in)-250(Co)19.8(L)-255.7(\()]TJ /F5 1 Tf 9.9931 0 TD (p)Tj /F6 1 Tf .6566 0 TD (<)Tj /F4 1 Tf .7084 0 TD [(0.)18.5(05)20.8(\).)-246.4(Tr)19.7(an)19.7(sc)14.1(ri)20.7(pt)20.7(io)20.7(n)-254.5(l)0(e)13.8(v)0(e)19.7(l)-254.7(ha)19.7(d)-254.5(n)0(o)]TJ -11.3581 -1.1174 TD [(si)20.9(gn)20.8(i)20.9(ca)12.8(nt)-152.1(di)20.7(ff)20.9(er)19.7(en)19.7(ce)-154.3(be)13.9(tw)19.8(ee)18.5(n)-162.4(t)0(h)20.7(e)-163.5(ge)19.7(ne)-153.1(of)]TJ /F5 1 Tf 16.2251 0 TD [(RU)18.8(NX)18.6(2)]TJ /F4 1 Tf 3.237 0 TD [(an)19.7(d)]TJ /F5 1 Tf 1.5897 0 TD [(SO)20(X9)]TJ /F4 1 Tf 2.4651 0 TD (in)Tj -23.5169 -1.1116 TD [(th)20.7(e)-296(O)0(M)-292(g)0(r)20.9(o)0(u)20.8(p)-300.6(in)-284.5(Co)14.1(L)-296(a)0(n)19.7(d)-300.6(DB)18.9(B/)19.7(Co)19.8(L)-296(s)0(c)14.1(a)0(f)19.7(f)0(o)20.9(l)0(d)20.7(s)-300.4(\()]TJ /F5 1 Tf 19.5196 0 TD (p)Tj /F6 1 Tf .6566 0 TD (>)Tj /F4 1 Tf .7085 0 TD [(0.)18.5(05)20.8(\).)-286.7(In)]TJ -20.8847 -1.1116 TD [(PM)-331.2(gr)20.9(ou)20.8(p,)-327.1(th)15(er)19.7(e)-336.3(w)0(a)13(s)-335(no)-330.5(ob)20.8(vi)14.9(ou)20.8(s)-340.7(d)0(i)20.7(f)0(f)20.9(e)0(r)19.7(e)0(n)19.7(c)0(e)-332.8(i)0(n)]TJ /F5 1 Tf 19.0301 0 TD [(OC)18.9(N)]TJ /F4 1 Tf 2.373 0 TD [(ex)19.7(pr)20.9(es)19.9(-)]TJ -21.4031 -1.1174 TD [(si)20.9(on)-319(am)18.5(on)20.8(g)-329.4(t)0(h)20.7(e)-330.6(th)20.7(re)13.9(e)-324.8(g)0(r)15.1(o)0(u)20.8(p)0(s)-318.8(o)0(f)-319(s)0(c)19.9(a)0(f)19.7(f)0(o)15.1(l)0(d)20.7(s)0(.)-315.4(T)0(h)13.9(e)-324.8(ex)13.9(pr)20.9(es)19.9(si)20.9(on)-319(of)]TJ /F5 1 Tf 0 -1.1116 TD [(RU)18.8(NX)18.6(2)]TJ /F4 1 Tf 3.5307 0 TD [(an)19.7(d)]TJ /F5 1 Tf 1.8892 0 TD [(SO)20(X9)]TJ /F4 1 Tf 2.7589 0 TD [(wa)18.8(s)-461.7(h)0(i)20.7(g)0(h)20.8(e)0(r)-446.8(i)0(n)-451.6(P)0(M)-446.4(g)0(r)20.9(o)0(u)20.8(p)-461.9(in)-445.8(DB)18.9(B/)19.7(Co)14(L)]TJ -8.1788 -1.1116 TD [(sc)19.9(af)19.7(fo)20.9(ld)15(s)-450.2(t)0(h)20.7(a)0(n)-441.1(t)0(h)15(e)-451.5(sa)19.9(me)-442.3(se)19.9(t)-450.5(o)0(f)-445.7(t)0(h)20.7(a)0(t)-441.2(i)0(n)-445.8(C)0(o)19.8(L)-451.5(an)19.7(d)-450.4(n)0(H)20(A)0(/)19.8(C)0(o)14(L)]TJ 0 -1.1174 TD [(sc)19.9(af)19.7(fo)20.9(ld)15(s,)-516.9(re)19.7(sp)21(ec)12.8(ti)20.6(ve)19.7(ly)20.7(.)-527.6(A)0(t)-521.6(d)0(a)19.7(y)-531(7,)-517.1(th)20.7(e)-532.2(e)0(x)19.7(p)0(r)20.9(e)0(s)19.9(s)0(i)15.1(o)0(n)-514.8(l)0(e)13.8(v)0(e)19.7(l)-525.4(of)]TJ /F5 1 Tf 0 -1.1116 TD [(CO)18.9(LI)21(A1)]TJ /F4 1 Tf 3.4846 0 TD [(in)-146.3(Co)19.8(L)-152(s)0(c)19.9(a)0(f)19.7(f)0(o)15.1(l)0(d)20.7(s)-150.7(wa)18.8(s)-156.4(h)0(i)20.7(g)0(h)20.8(e)0(r)-141.6(t)0(h)15(a)0(n)-141.6(t)0(h)20.7(a)0(t)-141.7(i)0(n)-146.3(n)0(H)20(A)0(/)19.8(C)0(o)19.8(L)-152.1(an)19.7(d)]TJ -3.4846 -1.1116 TD [(DB)18.9(B/)19.7(Co)19.8(L)-250(s)0(c)19.9(a)0(f)14(f)0(o)20.9(l)0(d)20.7(s)-248.6(in)-244.2(PM)-239.1(gr)20.9(ou)20.8(p)-254.5(\()]TJ /F5 1 Tf 13.8233 0 TD (p)Tj /F6 1 Tf .6566 0 TD (<)Tj /F4 1 Tf .7084 0 TD [(0.)18.5(05)20.8(\).)]TJ /F8 1 Tf 8.9663 0 0 8.9663 310.8472 369.0141 Tm (Discussion)Tj /F4 1 Tf 9.843 0 0 9.843 320.8251 352.5732 Tm [(Hydroxyapatite)-244.8(is)-244(a)-244.2(primary)-246.3(focus)-242.6(for)-245.1(synthetic)-248.6(bone)-241.7(graft)]TJ -1.0137 -1.1116 TD [(substitutes)-485.5(because)-484.5(they)-483.7(are)-483.6(osteoconductive)-487.9(and)-482.5(provide)]TJ T* [(sufcient)-346.2(mechanical)-352.4(strength.)]TJ 7.3823 0 0 6.5614 433.7007 335.0551 Tm (25)Tj 9.843 0 0 9.843 444.4724 330.6897 Tm [(Both)-345.4(nHA)-350.6(and)-344.3(DBB)-346.1(offer)]TJ -13.5756 -1.1116 TD [(several)-264.5(biological)-267.3(advantages)-269(as)-262.4(a)-261.4(bone)-264.8(analog,)-265.9(and)-263.7(the)-263.8(HA-)]TJ ET q 1 i 59.754 295.597 m 420.321 295.597 l 420.321 58.507 l 59.754 58.507 l W n q 360.926 0 0 237.09 59.754 58.507 cm /Im3 Do Q Q BT /F7 1 Tf 9.845 0 0 9.845 433.1337 267.8739 Tm [(FIG.)-290.7(4.)]TJ /F4 1 Tf 4.0713 0 TD (Immunouorescent)Tj -4.0713 -1.0135 TD [(staining)-337.7(for)-331.2(DAPI-stained)]TJ T* [(nuclei)-336.9(of)-330.2(cells)-335.7(in)-336.2(3D)-336.8(con-)]TJ T* [(structs)-336.3(after)-335.7(day)-332.4(1;)]TJ /F7 1 Tf 8.0217 0 TD (\(AC\))Tj /F4 1 Tf -8.0217 -1.0077 TD [(represent)-340.7(CoL,)-331(nHA/CoL,)]TJ 0 -1.0135 TD [(and)-257.5(DBB/CoL)-259.7(3D)-256.2(constructs,)]TJ T* [(respectively,)-337(indicating)-335.4(the)]TJ T* [(cellular)-340.2(3D)-331(spatial)-337.9(distribu-)]TJ 0 -1.0077 TD [(tion)-332.6(on)-336(day)-332.4(1\(40)]TJ /F6 1 Tf 7.0082 0 TD ()Tj /F4 1 Tf .7198 0 TD (\);)Tj /F7 1 Tf .9502 0 TD (\(DF\))Tj /F4 1 Tf -8.6781 -1.0135 TD [(are)-333.6(immunouorescent)]TJ T* [(staining)-337.7(for)-331.2(vinculin)-337.9(of)-330.2(cells)]TJ T* [(in)-255.5(CoL,)-256.1(nHA/CoL,)-256.1(and)-257.5(DBB/)]TJ 0 -1.0077 TD [(CoL)-333.4(3D)-336.8(constructs)-334.7(on)-336(day)-332.4(1)]TJ 0 -1.0135 TD [(after)-335.7(printing)-336.7(\(40)]TJ /F6 1 Tf 7.1867 0 TD ()Tj /F4 1 Tf .7198 0 TD [(\),)-332.6(respec-)]TJ -7.9065 -1.0135 TD [(tively.)-241.3(The)]TJ /F5 1 Tf 4.578 0 TD [(white)-243.6(boxes)]TJ /F4 1 Tf 4.9351 0 TD (in)Tj /F7 1 Tf 1.0193 0 TD (\(E\))Tj /F4 1 Tf -10.5324 -1.0077 TD (and)Tj /F7 1 Tf 1.7276 0 TD (\(F\))Tj /F4 1 Tf 1.5663 0 TD [(represented)-286.2(nHA)-281(and)]TJ -3.2939 -1.0135 TD [(the)-332.6(degradation)-337.2(particles)-341(of)]TJ T* [(DBB,)-239.5(respectively.)-244.9(Scale)-244.6(bar:)]TJ T* (10)Tj /F10 1 Tf 1.002 0 TD (m)Tj /F4 1 Tf .5528 0 TD [(m.)-333.8(Color)-334.4(images)-331.9(are)]TJ -1.5548 -1.0077 TD [(available)-336.6(online.)]TJ ET q 1 i 60.321 732.245 m 298.261 732.245 l 298.261 552.132 l 60.321 552.132 l W n q 238.023 0 0 180.113 60.321 552.132 cm /Im4 Do Q Q BT /F7 1 Tf 9.845 0 0 9.845 59.7543 535.3511 Tm [(FIG.)-480.7(3.)]TJ /F4 1 Tf 4.2613 0 TD [(Youngs)-474(moduli)-473.9(of)-479.9(CoL,)-474.9(nHA/CoL,)-480.7(and)-476.3(DBB/)]TJ -4.2613 -1.0135 TD [(CoL)-362.2(group)-362(\(*)]TJ /F5 1 Tf 5.6722 0 TD (p)Tj /F6 1 Tf .668 0 TD (<)Tj /F4 1 Tf .7198 0 TD [(0.05;)-358.8(**)]TJ /F5 1 Tf 3.3918 0 TD (p)Tj /F6 1 Tf .6622 0 TD (<)Tj /F4 1 Tf .7198 0 TD [(0.01\).)-362(The)-362.3(Youngs)-364.6(moduli)-358.7(of)]TJ -11.8338 -1.0135 TD [(nHA/CoL)-379.5(was)-373.3(signicantly)-377.4(stranger)-373.1(then)-373.9(CoL)-373.7(\(**)]TJ /F5 1 Tf 20.8229 0 TD (p)Tj /F6 1 Tf .6622 0 TD (<)Tj /F4 1 Tf .7198 0 TD (0.01\))Tj -22.2049 -1.0135 TD [(and)-343.9(DBB/CoL)-340.3(scaffold)-344.3(\(*)]TJ /F5 1 Tf 10.6418 0 TD (p)Tj /F6 1 Tf .668 0 TD (<)Tj /F4 1 Tf .7198 0 TD [(0.05\),)-338.9(respectively;)-340.6(DBB/CoL)]TJ -12.0296 -1.0077 TD [(was)-338.8(stranger)-332.7(then)-339.3(CoL)-333.4(scaffold)-332.7(\(*)]TJ /F5 1 Tf 14.0221 0 TD (p)Tj /F6 1 Tf .668 0 TD (<)Tj /F4 1 Tf .7198 0 TD (0.05\).)Tj /F8 1 Tf 8.9663 0 0 8.9663 59.7543 749.7069 Tm [(A)-337.9(COMPARATIVE)-335(EVALUATION)]TJ 52.4612 0 TD (1267)Tj ET endstream endobj 76 0 obj <>stream Adobed         s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjzm!1AQa"q2#BRbr3$4CS%cs5DT &6E'dtU7()󄔤euFVfvGWgw8HXhx9IYiy*:JZjz?j~^& p^yRHEڸleT76}(AZ˯6لSkִȮs7M DLjXM-XȞN,T|Y<.)e.vٓq[LB(c1:B'{Lŝ{ 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.4992 0 TD (+)Tj /F4 1 Tf 9.843 0 0 9.843 378.0849 418.2802 Tm [(as)-343(well)-340.8(as)-343(crosslinking)-342.7(of)-342(using)-339.5(glutaralde-)]TJ -6.6064 -1.1116 TD [(hyde,)-354.6(which)-347.5(can)-351.2(increase)-353.2(the)-350.2(mechanics)-349.8(of)-347.7(collagen)-352.4(by)-347.8(in-)]TJ T* [(creasing)-265.6(its)-256.8(resistance)-263.2(to)-255.8(bril)-259(rotation.)]TJ 7.3823 0 0 6.5614 467.0361 400.7621 Tm (23,34)Tj 9.843 0 0 9.843 486.1983 396.3968 Tm [(Collagen)-260.1(plays)-260(a)]TJ -17.5901 -1.1174 TD [(role)-397.3(in)-394(crystallizing)-398.4(and)-390.4(mineralizing)-398.6(templates)-395(during)-398.4(the)]TJ 0 -1.1116 TD [(process)-211.2(of)-198(HA)-205.5(crystallization,)-209.5(and)-200.3(has)-205.9(the)-206.2(function)-200.3(of)-203.8(guiding)]TJ T* [(the)-246.5(distribution)-244(of)-238.3(HA)-240.1(particles)-249.7(along)-237.2(the)-246.5(surface)-241.3(of)-244.1(collagen)]TJ 0 -1.1174 TD (bers.)Tj 7.3823 0 0 6.5614 337.3795 356.9385 Tm (44)Tj 9.843 0 0 9.843 346.8471 352.5164 Tm [(Meanwhile,)-214.7(the)-211.9(combination)-217.7(of)-209.5(collagen)-214.1(and)-211.8(Ca)]TJ 7.3823 0 0 6.5614 534.6707 356.9385 Tm (2)Tj /F6 1 Tf .4992 0 TD (+)Tj /F4 1 Tf 9.843 0 0 9.843 544.4786 352.5164 Tm (in)Tj -23.5111 -1.1116 TD [(the)-275.3(material)-278.8(have)-277.4(possibility)-271.6(to)-278.8(mineralize)-276.4(the)-275.3(collagen)-277.5(ber)]TJ T* [(and)-211.8(thus)-205.9(may)-207.3(increase)-209.2(the)-206.2(Youngs)-210(modulus)-204.7(of)-209.5(the)-206.2(composite)]TJ T* (scaffolds.)Tj 7.3823 0 0 6.5614 351.1558 324.0566 Tm (45)Tj 9.843 0 0 9.843 361.7574 319.6913 Tm [(nHA)-327.6(has)-326.8(a)-324.8(smaller)-328.1(particle)-329.4(size)-329.2(than)-328.2(DBB,)-332.2(and)]TJ -4.9476 -1.1174 TD [(therefore)-307.2(has)-303.8(a)-301.8(larger)-302.7(surface)-304.7(area,)-305(making)-302.9(the)-304.1(Ca)]TJ 7.3823 0 0 6.5614 513.2408 313.0582 Tm (2)Tj /F6 1 Tf .5069 0 TD (+)Tj /F4 1 Tf 9.843 0 0 9.843 524.0125 308.6928 Tm [(easy)-300.3(to)]TJ -21.4319 -1.1116 TD [(dissolve)-436.1(into)-430.9(collagen.)-436.5(Hence,)-433.8(nHA/CoL)-438(has)-430.5(higher)-434.1(com-)]TJ T* [(pressive)-264.3(modulus)-262.3(than)-264.9(the)-258(other)-266(two)-257.7(groups.)]TJ 1.0137 -1.1174 TD [(Af)20(te)13.8(r)-248.7(t)0(h)14.9(e)-249.9(co)13.9(m)15(p)0(o)15.1(s)0(i)20.9(t)0(e)-245.4()62.3(b)20.9(io)15(in)20.7(k)72.7()-254.5(w)0(a)18.8(s)-254.3(pr)20.9(in)15(te)19.6(d)-248.8(i)0(n)15(t)0(o)-244.2(3)0(D)-239.2(s)0(c)14.1(a)0(f)19.7(f)0(o)20.9(l)0(d)]TJ -1.0137 -1.1116 TD [(by)-658.8(th)15(e)-664.6(3)0(D)-659.7(b)0(i)14.9(o)0(p)20.8(r)0(i)20.7(n)0(t)20.7(e)0(r)19.7(,)-671.6(a)-670.4(s)0(p)21(e)0(c)18.5(i)0()20.9(c)-670.4(ar)19.7(ch)19.7(it)20.6(ec)12.8(tu)20.7(re)-659.9(wa)18.8(s)-669(b)0(u)20.8(i)0(l)20.6(t)]TJ ET q 1 i 191.622 239.357 m 552.189 239.357 l 552.189 58.734 l 191.622 58.734 l W n q 360.926 0 0 180.624 191.622 58.734 cm /Im5 Do Q Q BT /F7 1 Tf 9.845 0 0 9.845 62.022 224.7873 Tm [(FIG.)-336.7(6.)]TJ /F4 1 Tf 4.1174 0 TD [(AL)19.1(P)-323.3(s)0(t)21(a)0(i)19.7(n)0(i)20.9(n)0(g)-312.9(o)0(f)]TJ -4.1174 -1.0135 TD [(hB)20.1(MS)20.4(Cs)-313.8(on)-312.9(sc)14.3(af)19.9(fo)21(ld)20.9(s)-323.3(7)-323.5(da)19.9(ys)]TJ T* [(af)19.9(te)19.7(r)-323.5(s)0(e)14.3(e)0(d)19.9(i)0(n)20.9(g)-323.5(\(1)21(00)]TJ /F6 1 Tf 7.4112 0 TD ()Tj /F4 1 Tf .7083 0 TD (\).)Tj /F7 1 Tf .8983 0 TD [(\(A)20.2(C)20.2(\))]TJ /F4 1 Tf -9.0179 -1.0077 TD [(re)19.9(pr)21(es)14.3(en)19.9(t)-317.9(c)0(e)18.7(l)0(l)14.9(s)-317.5(on)-312.9(Co)20.1(L,)-310.7(nH)20.2(A/)]TJ 0 -1.0135 TD [(Co)20.1(L)-272.8(a)0(n)19.9(d)-265.9(DB)19.2(B/)-262.3(Co)20.1(L)-272.8(s)0(c)20(a)0(f)19.9(f)0(o)21(l)0(d)20.9(s)]TJ T* [(in)-284.3(pr)21(ol)20.9(if)20.9(er)14.1(at)19.7(io)20.9(n)-294.7(m)15.1(ed)14.1(iu)20.9(m)-290.1(\()0(P)21.2(M)0(\))]TJ T* [(di)20.9(d)-323.5(n)0(o)21.1(t)-323.6(di)20.9(sp)15.5(la)19.7(y)-323.5(s)0(i)21(g)0(n)21.1(i)0()21(c)0(a)18.7(n)0(t)]TJ 0 -1.0077 TD [(AL)19.1(P)-323.3(s)0(t)21(a)0(i)19.7(n)0(i)15.1(n)0(g)21.1(,)]TJ /F7 1 Tf 5.8564 0 TD [(\(D)20.2(F)19.9(\))]TJ /F4 1 Tf 2.7814 0 TD [(re)14.1(-)]TJ -8.6378 -1.0135 TD [(pr)21.1(es)20(en)14.1(t)-323.6(c)0(e)18.7(l)0(l)20.7(s)-323.3(on)-312.9(Co)20.1(L,)-310.7(nH)14.5(A/)]TJ T* [(Co)20.1(L)-272.8(a)0(n)19.9(d)-265.9(DB)19.2(B/)-262.3(Co)20.1(L)-272.8(s)0(c)20(a)0(f)19.9(f)0(o)21(l)0(d)20.9(s)]TJ 0 -1.0077 TD [(in)-313.1(os)21.2(te)19.7(og)15.3(en)19.9(ic)19.7(-i)20.9(nd)21.1(uc)19.9(ti)20.7(on)-312.9(me)18.7(d-)]TJ 0 -1.0135 TD [(iu)20.9(m)-324.6(\()0(O)20.2(M)0(\))-313.8(s)0(h)21.2(o)0(w)14.5(e)0(d)-314.1(p)0(o)21.1(s)0(i)21(t)0(i)20.7(v)0(e)]TJ T* [(AL)19.1(P)-323.3(s)0(t)21(a)0(i)19.7(n)0(i)15.1(n)0(g)21.1(.)-320.1(Th)19.9(e)-324.6(b)0(r)21(o)0(w)14.5(n)]TJ T* [(sp)21.2(in)20.9(dl)15.1(e)-324.6(m)15.1(or)15.3(ph)21.1(ol)20.9(og)21.1(y)-323.5(i)0(n)]TJ /F5 1 Tf 9.3807 0 TD [(re)20(d)]TJ -9.3807 -1.0077 TD [(da)21.1(sh)21.2(ed)-216.2(bo)21.1(x)]TJ /F7 1 Tf 4.6587 0 TD [(\(D)20.2(F)19.9(\))]TJ /F4 1 Tf 2.6835 0 TD [(re)19.9(pr)21.1(es)20.1(en)19.9(te)19.7(d)]TJ -7.3421 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[(si)15.1(on)-532.1(of)-532.1(th)20.7(e)-543.7(t)0(i)20.6(p)-542.5(mo)19.6(ld)-532.2(an)19.7(d)-542.5(n)0(o)20.8(z)0(z)18.5(l)0(e)19.6(.)-544.8(In)20.9(te)19.6(rn)20.9(al)13.8(ly)20.7(,)-539.1(t)0(h)20.7(e)-543.7(sc)14.1(af)19.7(fo)20.9(ld)]TJ 0 -1.1116 TD [(pr)15.1(es)19.9(en)19.7(te)19.6(d)-490.7(t)0(h)15(e)-491.8(ty)20.7(pi)20.7(ca)18.5(l)-496.6(c)0(h)19.7(a)0(r)19.7(a)0(c)18.5(t)0(e)19.6(r)0(i)15(s)0(t)20.9(i)0(c)19.6(s)-490.5(of)-486(ca)18.5(nc)19.7(el)19.5(lo)20.7(us)-485.8(bo)20.8(ne)19.7(,)-493(a)]TJ T* [(ho)15.1(ne)19.7(yc)19.7(om)19.6(b)-484.9(m)0(i)19.5(c)0(r)19.7(o)0(s)15.3(t)0(r)20.7(u)0(c)19.7(t)0(u)20.7(r)0(e)-481.4(e)0(v)19.7(e)0(n)19.7(l)0(y)-480.4(d)0(i)20.7(s)0(t)20.9(r)0(i)20.7(b)0(u)15.1(t)0(e)19.6(d)-484.9(wi)19.8(th)-480.4(10)20.8(0)]TJ /F10 1 Tf 22.9697 0 TD (m)Tj /F4 1 Tf .5414 0 TD (m)Tj -23.5111 -1.1174 TD [(po)15.1(re)19.7(s.)-217.4(Th)19.6(e)-226.9(p)0(o)20.8(r)0(e)19.7(s)-225.5(ar)19.7(e)-226.9(a)0(s)14.1(s)0(u)21(m)0(e)18.4(d)-225.7(to)-215.4(be)-216.5(cr)14(ea)18.5(te)19.6(d)-225.7(b)0(y)-215.3(t)0(h)20.7(e)-226.9(su)15.3(bl)20.7(im)19.5(at)19.6(io)20.7(n)]TJ 0 -1.1116 TD [(of)-474.5(th)15(e)-480.3(s)0(o)15.3(l)0(v)20.7(e)0(n)19.7(t)-485(du)20.8(ri)20.7(ng)-474.5(ly)20.7(op)20.8(hi)14.9(li)20.6(za)18.5(ti)20.6(on)20.8(.)]TJ 7.3823 0 0 6.5614 207.9495 236.296 Tm (46)Tj 9.843 0 0 9.843 220.0251 231.9306 Tm -.0206 Tc [(T)-20.6(h)-1(e)-506.7(h)-20.6(ig)-20.6(h-)-20.6(re)-20.6(so)-20.6(l)-5.6(u)-20.6(ti)-20.6(on)]TJ -16.2827 -1.1116 TD 0 Tc [(SE)19.8(M)-572.2(i)0(m)19.5(a)0(g)19.7(e)0(s)-561.9(o)0(f)-560.9(n)0(H)20(A)0(/)19.8(C)0(o)19.8(L)-572.5(re)19.7(e)19.8(ct)19.5(ed)-562.1(th)20.7(e)-566.7(u)0(n)15.1(i)0(f)20.7(o)0(r)20.9(m)-566.8(pa)13.9(rt)20.7(ic)19.6(le)]TJ T* [(di)14.9(st)20.9(ri)20.7(bu)20.8(ti)14.8(on)-497.5(of)-503.3(nH)20(A,)-506.5(wh)20(ic)19.6(h)-513.7(r)0(e)19.7(s)0(u)21(l)0(t)20.6(e)0(d)-504.5(i)0(n)-503.4(a)-509.1(mo)13.9(re)-498.7(in)20.7(te)13.8(gr)20.9(at)19.5(ed)]TJ 0 -1.1174 TD [(st)15.1(ru)20.9(ct)19.5(ur)20.9(e.)-224.6(As)-221.7(to)-221.2(th)20.7(e)-232.6(D)0(B)18.9(B)-232.5(pa)19.7(rt)15(ic)19.6(le)19.6(s,)-223.2(be)19.7(ca)18.5(us)21(e)-232.6(o)0(f)-221(t)0(h)20.7(e)-232.6(pa)19.7(rt)15(ic)19.6(le)-222.4(si)20.9(ze)]TJ 0 -1.1116 TD [(an)13.9(d)-306.4(t)0(h)20.7(e)-307.5(in)20.7(te)19.6(rn)15.1(al)-297.2(po)20.8(re)19.7(s,)-298.1(BD)18.9(D/)19.8(Co)14(L)-307.6(g)0(r)20.9(o)0(u)20.8(p)-306.4(pr)20.9(es)14.1(en)19.7(te)19.6(d)-306.4(a)-307.5(po)20.8(ro)15.1(si)20.9(ty)]TJ T* [(st)15.1(ru)20.9(ct)19.5(ur)20.9(e,)-374.3(in)15(cl)19.5(ud)20.8(in)20.7(g)-387(t)0(h)20.7(e)-382.4(mi)19.5(cr)19.7(op)15.1(or)20.9(es)-371.8(in)15(si)20.9(de)-372(th)20.7(e)-388.2(m)0(a)18.4(t)0(e)19.6(r)0(i)20.7(a)0(l)19.5(s)-386.8(an)19.7(d)]TJ 0 -1.1174 TD [(th)15(e)-319(m)0(a)18.4(c)0(r)14(o)0(p)20.8(o)0(r)20.9(e)0(s)-314.2(f)0(a)19.7(b)0(r)20.9(i)0(c)13.8(a)0(t)19.5(e)0(d)-314.4(b)0(y)-313.2(t)0(h)20.7(e)-324.8(3D)-314.1(pr)20.9(in)20.7(te)19.6(rs)15.3(.)]TJ 1.0137 -1.1116 TD [(A)-445.5(series)-446.4(of)-445.7(studies)-446.5(have)-444.5(demonstrated)-450(that)-443.6(the)-442.3(mineral)]TJ -1.0137 -1.1116 TD [(properties,)-385.4(including)-385.8(solubility,)-383.5(chemical)-383.6(composition,)-381(and)]TJ 0 -1.1174 TD [(surface)-454.4(morphology)-444.3(were)-451.1(important)-444.6(factors)-453.4(for)-446.7(regulating)]TJ 0 -1.1116 TD [(diverse)-407.4(cell)-404.4(behaviors.)]TJ 7.3823 0 0 6.5614 151.37 126.7086 Tm (3638)Tj 9.843 0 0 9.843 173.8204 122.3433 Tm [(The)-403.1(different)-409.8(properties)-405(of)-399.6(the)]TJ -11.5885 -1.1116 TD [(two)-321.1(inorganic)-323.5(parameters)-327.8(may)-316.7(have)-323.5(a)-319(diverse)-321(effect)-326.9(on)-319(cell)]TJ 0 -1.1174 TD [(behavior.)-354.5(The)-351.2(hBMSCs)-354.8(have)-352.3(obvious)-353.2(multipotent)-349.2(differen-)]TJ 0 -1.1116 TD [(tiation)-516.2(abilities)-513.8(and)-511.3(are)-512.4(the)-511.4(most)-512.4(common)-511.3(cells)-509(in)-514.9(bone)]TJ T* [(tissue)-382(engineering.)]TJ 7.3823 0 0 6.5614 135.0425 82.885 Tm (7,22,23)Tj 9.843 0 0 9.843 160.9511 78.5197 Tm [(In)-376.5(this)-373.1(study,)-377.5(hBMSCs)-377.9(were)-381.9(used)]TJ -10.2811 -1.1116 TD [(to)-434.3(detect)-436.6(the)-430.8(effect)-436.4(of)-428.4(the)-430.8(two)-436.3(inorganic)-432.9(materials)-435.2(on)-428.4(the)]TJ 0 -1.1174 TD [(osteogenic)-337.1(differentiation.)]TJ 26.5234 28.9483 TD -.0203 Tc [(T)-5.3(h)-20.3(e)-421.1(F)-20.3(I)-5(T)-20.3(C-)-20.3(ph)-20.3(al)-20.3(lo)-20.3(i)-5.4(d)-20.3(in)-20.3(/P)-20.3(I)-419.7(s)-20.3(ta)-20.3(in)-20.3(i)-5.4(n)-20.3(g)-419.9(i)-20.3(nt)-20.3(ui)-20.3(ti)-20.3(v)-5.3(e)-20.3(ly)-430.3(d)-20.3(i).4(s)-20.3(p).7(l)-20.3(a)-.8(y)-20.3(e)-.6(d)-430.3(t)-20.3(h)-5.4(e)]TJ -1.0137 -1.1116 TD 0 Tc [(gr)20.9(ow)20(th)-301.8(an)19.7(d)-312.1(d)0(i)20.7(s)0(t)15.1(r)0(i)20.7(b)0(u)20.8(t)0(i)20.6(o)0(n)-301.7(o)0(f)-301.7(c)0(e)18.5(l)0(l)20.6(s)-311.9(on)-301.7(3D)-302.6(bi)20.7(op)20.8(ri)15(nt)20.7(ed)-302.9(co)19.7(ns)21(tr)20.7(uc)13.9(ts)20.9(.)]TJ T* [(Th)19.6(e)-226.9(n)0(u)15.1(m)0(b)19.6(e)0(r)-216.4(o)0(f)-221(c)0(e)18.5(l)0(l)20.6(s)-231.3(in)-221.2(th)20.7(e)-232.6(c)0(o)19.7(l)0(l)20.6(a)0(g)19.7(e)0(n)-222.2(s)0(c)19.9(a)0(f)19.7(f)0(o)20.9(l)0(d)-221.2(g)0(r)20.9(o)0(u)15.1(p)-225.7(wa)18.8(s)-231.3(h)0(i)20.7(g)0(h)20.8(e)0(r)]TJ 0 -1.1174 TD [(th)20.7(an)-406.5(th)20.7(at)-406.7(of)-405.3(th)20.7(e)-417(o)0(t)20.7(h)0(e)19.7(r)-415.8(tw)14.1(o)-410(g)0(r)15.1(o)0(u)20.8(p)0(s)21(.)-418.1(Th)19.6(e)-411.2(t)0(w)14.1(o)-415.8(se)19.9(ts)-405.3(of)-405.3(pr)20.9(in)20.7(te)19.5(d)]TJ 0 -1.1116 TD [(sc)19.9(af)19.7(fo)20.9(ld)15(s)-237(h)0(a)19.7(v)0(e)-228(l)0(a)19.5(r)0(g)20.9(e)0(r)-228(p)0(o)20.8(r)0(e)-228(s)0(i)20.9(z)0(e)-229.1(t)0(h)20.7(a)0(n)-228(t)0(h)20.7(e)-238.4(co)19.7(ll)20.6(ag)19.7(en)-228(gr)15.1(ou)20.8(p,)-223.4(wh)20(ic)19.5(h)]TJ T* [(ca)18.5(us)21(ed)-366.2(a)-376.6(l)0(o)15(w)0(e)18.8(r)-375.4(se)19.9(ed)19.7(in)15(g)-375.5(e)0(f)19.7()0(c)19.8(i)0(e)19.6(n)0(c)19.7(y)0(,)-367.4(c)0(o)19.7(n)0(s)15.3(i)0(s)20.9(t)0(e)19.5(n)0(t)-365.2(w)0(i)19.8(t)0(h)-370.9(o)0(t)20.7(h)0(e)19.7(r)-375.5(re)19.7(-)]TJ 0 -1.1174 TD [(se)19.9(ar)19.7(ch)19.7(.)]TJ 7.3823 0 0 6.5614 337.833 280.1763 Tm (41)Tj 9.843 0 0 9.843 348.718 275.7543 Tm [(As)20.2(se)14.1(ss)21.2(me)18.4(nt)-353.7(of)-359.3(th)20.7(e)-365.1(b)0(i)14.9(o)0(s)21(a)0(f)19.7(e)0(t)19.5(y)-364(of)-359.3(sc)19.9(af)19.7(fo)15.1(ld)20.7(s)-363.8(i)0(s)-359.2(o)0(f)-353.5(p)0(r)20.9(i)0(-)]TJ -3.8475 -1.1116 TD [(ma)18.4(ry)-762.4(im)19.5(po)20.8(rt)20.7(an)19.7(ce)12.8(.)-769.5(V)0(i)19.8(n)0(c)19.7(u)0(l)14.9(i)0(n)-762.6(s)0(t)20.9(a)0(i)19.5(n)0(i)20.7(n)0(g)-768.2(a)0(n)19.7(d)-772.9(CC)18.8(K-)20(8)-772.9(a)0(s)19.9(s)0(a)19.9(y)]TJ T* [(de)19.7(mo)19.6(ns)21(tr)20.7(at)13.8(ed)-360.5(th)20.7(at)-366.4(th)20.7(e)-370.9(b)0(i)20.7(o)0(p)15.1(r)0(i)20.7(n)0(t)20.7(e)0(d)-360.5(s)0(c)14.1(a)0(f)19.7(f)0(o)20.9(l)0(d)20.7(s)-375.3(we)18.8(re)-360.4(no)15.1(nt)20.7(ox)20.8(ic)-360.6(to)]TJ 0 -1.1174 TD [(hB)19.8(MS)20.1(Cs)]TJ /F5 1 Tf 3.9684 0 TD [(in)-353.7(vi)13.8(tr)20.9(o)]TJ /F4 1 Tf 2.9835 0 TD [(.)-366.3(V)0(i)19.8(n)0(c)19.7(u)0(l)20.7(i)0(n)-353.7(s)0(t)15.1(a)0(i)19.6(n)0(i)20.7(n)0(g)-353.5(r)0(e)19.7(s)0(u)15.3(l)0(t)20.6(s)-363.8(pr)20.9(ov)15.1(ed)-354.7(th)20.7(at)-354.8(vi)20.7(n-)]TJ -6.952 -1.1116 TD [(cu)19.7(li)20.6(n)-312.1(w)0(a)13(s)-311.9(ex)19.7(pr)20.9(es)14.1(se)19.9(d)-312.1(a)0(r)19.7(o)0(u)20.8(n)0(d)-307.5(a)0(l)19.5(m)0(o)19.6(s)0(t)-301.6(a)0(l)13.8(l)-312.3(ce)18.5(ll)20.6(s,)-303.8(wh)20(ic)13.8(h)-312.1(i)0(n)20.7(d)0(i)20.7(c)0(a)18.5(t)0(e)19.5(d)]TJ T* [(th)20.7(at)-372.1(hB)14.1(MS)20.1(Cs)-371.7(co)19.7(ul)14.9(d)-381.2(s)0(h)21(o)0(w)-377.4(a)0(d)19.7(h)0(e)19.7(s)0(i)20.9(v)0(e)-377.7(g)0(r)20.9(o)0(w)20(t)0(h)-370.9(i)0(n)-376.7(b)0(o)20.8(t)0(h)-376.7(t)0(h)20.7(e)-382.4(co)19.7(l-)]TJ T* [(la)19.6(ge)19.7(n)-254.5(m)0(a)18.4(t)0(r)20.7(i)0(x)-244.2(a)0(n)19.7(d)-254.5(th)20.7(e)-255.7(c)0(o)19.7(m)0(p)19.6(o)0(s)21(i)0(t)14.8(e)-249.9(ma)18.4(tr)15(ix)-238.5(an)13.9(d)-248.8(i)0(l)14.8(l)0(u)20.7(s)0(t)20.9(r)0(a)19.7(t)0(e)19.6(d)-254.5(th)20.7(at)-245.4(th)14.9(e)]TJ 0 -1.1174 TD [(ad)19.7(he)19.7(si)20.9(on)-370.8(ab)19.7(il)20.6(it)20.6(y)-381.2(w)0(a)18.8(s)-375.3(si)20.9(mi)19.5(la)13.8(r)-375.4(o)0(n)-365.1(a)0(l)13.8(l)-375.6(th)20.7(re)19.7(e)-382.4(t)0(y)20.7(p)0(e)19.7(s)-375.3(of)-370.8(sc)19.9(af)19.7(fo)20.9(ld)14.9(s.)]TJ 0 -1.1116 TD [(Th)19.6(e)-468.8(C)0(C)18.8(K)0(-)20(8)-473.4(as)19.9(sa)19.9(y)-467.6(r)0(e)19.7(s)0(u)15.3(l)0(t)20.6(s)-467.4(in)20.7(di)14.9(ca)18.5(te)19.6(d)-467.6(t)0(h)20.7(a)0(t)-464.3(t)0(h)20.7(e)-468.8(ce)18.5(ll)20.6(s)-473.2(s)0(t)20.9(i)0(l)20.6(l)-467.8(ha)19.7(d)]TJ T* [(pr)20.9(ol)20.7(if)20.7(er)14(at)19.5(iv)20.7(e)-290.2(c)0(a)18.5(p)0(a)19.7(c)0(i)19.5(t)0(y)-278.8(i)0(n)-278.8(t)0(h)20.7(e)-290.2(bi)20.7(op)15.1(ri)20.7(nt)20.7(ed)-279.8(ma)18.4(tr)20.7(ix)-278.8(ma)18.5(te)19.5(ri)20.7(al)13.8(.)-285.6(A)0(f)20(t)0(e)19.5(r)]TJ 0 -1.1174 TD [(3)-364(d)0(a)19.7(y)0(s)-353.3(o)0(f)-359.3(i)0(n)20.7(c)0(u)19.7(b)0(a)19.7(t)0(i)20.6(o)0(n)15.1(,)-360.5(th)20.7(e)-370.9(c)0(e)18.5(l)0(l)20.6(s)-363.8(in)-353.7(co)13.9(ll)20.6(ag)19.7(en)-354.7(gr)20.9(ou)20.8(p)-369.7(h)0(a)19.7(d)-364(a)-365.1(s)0(i)20.9(g)0(-)]TJ 0 -1.1116 TD [(ni)20.7(c)19.8(an)19.7(t)-559.9(p)0(r)20.9(o)0(l)14.9(i)0(f)20.7(e)0(r)19.7(a)0(t)19.5(i)0(v)20.7(e)-561(ef)19.7(fe)14(ct)-544.9(co)13.9(mp)19.6(ar)19.7(ed)-550.5(wi)19.8(th)-549.5(th)20.7(e)-561(c)0(o)19.7(m)0(p)19.6(o)0(s)21(i)0(t)20.6(e)]TJ T* [(gr)20.9(ou)20.8(p.)-309.8(Th)19.6(e)-319(d)0(i)20.7(f)0(f)15.1(e)0(r)19.7(e)0(n)19.7(c)0(e)-309.8(m)0(i)19.5(g)0(h)20.8(t)-318(be)-308.6(ca)12.8(us)21(ed)-308.6(by)-307.5(th)20.7(e)-319(l)0(o)15(w)0(e)18.8(r)-317.8(se)19.9(ed)19.7(in)15(g)]TJ 0 -1.1174 TD [(ef)19.7(c)19.8(ie)19.5(nc)19.7(y)-484.9(i)0(n)-474.6(t)0(h)20.7(e)-480.3(nH)20(A/)14.1(Co)19.8(L)-480.3(a)0(n)19.7(d)-484.9(DB)18.9(B/)19.7(Co)19.8(L)-486.1(g)0(r)20.9(o)0(u)20.8(p)-484.9(as)-469.7(me)18.4(n-)]TJ 0 -1.1116 TD [(ti)20.6(on)20.8(ed)-216.5(ab)19.7(ov)20.8(e.)-218.8(Mo)19.9(re)19.7(ov)20.8(er)19.7(,)-228.1(t)0(h)20.7(e)-226.9(io)20.7(ns)-215.1(fr)20.9(om)-216.5(di)20.7(si)20.9(nt)14.9(eg)19.7(ra)19.7(te)19.6(d)-225.7(i)0(n)20.7(o)0(r)20.9(g)0(a)19.7(n)0(i)14.9(c)]TJ T* [(ma)18.4(te)19.6(ri)20.7(al)19.6(s,)-275(su)21(ch)-274.1(as)-279.6(Ca)]TJ 7.3823 0 0 6.5614 393.7322 115.7669 Tm (2)Tj /F6 1 Tf .4838 0 TD (+)Tj /F4 1 Tf 9.843 0 0 9.843 404.1637 111.4016 Tm [(an)13.9(d)-283.3(P)0(O)]TJ 7.3823 0 0 6.5614 433.3605 109.7575 Tm (4)Tj .4838 .9159 TD (3-)Tj 9.843 0 0 9.843 445.8329 111.4016 Tm [(an)19.7(d)-283.3(s)0(o)-272.7(o)0(n)20.8(,)-285.7(mi)19.5(gh)20.8(t)-283.4(i)0(n)20.7()0(u)21(e)0(n)13.9(c)0(e)]TJ -13.7139 -1.1174 TD [(th)20.7(e)-434.2(O)0(D)-430.2(v)0(a)19.7(l)0(u)20.7(e)0(s)19.9(.)-435.4(AL)18.8(P)-438.7(s)0(t)20.9(a)0(i)19.5(n)0(i)20.7(n)0(g)-428.4(i)0(s)-428.4(a)0(n)-423.8(i)0(n)20.7(d)0(i)20.7(c)0(a)12.8(t)0(o)20.7(r)-433(re)19.7(e)14.1(ct)19.5(in)20.7(g)-433.1(t)0(h)20.7(e)]TJ 0 -1.1116 TD [(ab)19.7(il)20.6(it)20.6(y)-663.5(o)0(f)-653(o)0(s)21(t)0(e)19.6(o)0(b)20.8(l)0(a)13.8(s)0(t)20.9(i)0(c)-654.3(d)0(i)20.7(f)0(f)20.9(e)0(r)19.7(e)0(n)19.7(t)0(i)14.8(a)0(t)19.5(i)0(o)20.7(n)-663.5(of)-647.3(hB)19.8(MS)14.3(Cs)20(.)-660(A)0(f)20(t)0(e)19.5(r)]TJ T* [(7)-248.8(d)0(a)19.7(y)0(s)-243.9(i)0(n)-244.2(O)0(M)19.1(,)-251.1(hB)19.8(MS)20.1(Cs)-244.9(sh)21(ow)20(ed)-245.3(po)20.8(si)20.9(ti)20.6(ve)-245.3(AL)18.8(P)-248.6(s)0(t)15.1(a)0(i)19.5(n)0(i)20.7(n)0(g)20.8(,)-251.1(wh)20(il)14.8(e)]TJ T* [(th)20.7(e)-336.3(P)0(M)-331.2(g)0(r)20.9(o)0(u)20.8(p)-335.2(di)20.7(d)-340.9(n)0(o)20.8(t)-335.3(sh)15.3(ow)-325.6(si)20.9(gn)20.8(i)15.1(ca)18.5(nt)-324.9(po)20.8(si)15.1(ti)20.6(ve)-325.9(te)19.6(st)15.1(s)-335(r)0(e)19.7(s)0(u)21(l)0(t)14.8(s)0(,)]TJ 0 -1.1174 TD [(co)19.7(ns)21(is)20.9(te)13.8(nt)-313.4(wi)19.8(th)-313.4(th)20.7(e)-324.8(p)0(r)20.9(e)0(v)19.7(i)0(o)20.7(u)0(s)-318.8(r)0(e)19.7(p)0(o)20.8(r)0(t)20.7(.)]TJ 7.3823 0 0 6.5614 449.9149 60.9449 Tm -.0162 Tc (23)Tj ET q 360.926 0 0 370.035 59.754 361.984 cm /Im7 Do Q BT /F7 1 Tf 9.845 0 0 9.845 433.1337 598.6203 Tm 0 Tc [(FIG.)-336.7(7.)]TJ /F4 1 Tf 4.1174 0 TD [(Expression)-334.5(of)]TJ /F5 1 Tf -4.1174 -1.0135 TD (RUNX2)Tj /F7 1 Tf 3.4033 0 TD (\(A\))Tj /F5 1 Tf 1.3821 0 TD [(,)-285.5(OCN)]TJ /F7 1 Tf 2.8735 0 TD (\(B\))Tj /F4 1 Tf 1.336 0 TD (,)Tj /F5 1 Tf .5355 0 TD (SOX9)Tj /F7 1 Tf -9.5304 -1.0077 TD (\(C\))Tj /F5 1 Tf 1.3878 0 TD (,)Tj /F4 1 Tf .5816 0 TD (and)Tj /F5 1 Tf 1.7794 0 TD (COL)Tj /F7 1 Tf 2.2746 0 TD (\(D\))Tj /F4 1 Tf 1.716 0 TD [(genes)-335.4(in)]TJ -7.7395 -1.0135 TD [(hBMSCs)-336.6(cultured)-333.1(in)-336.2(PM)-330.9(or)]TJ T* [(OM.)-335.2(\()49.8(*)48.9()-329.2(represent)]TJ /F5 1 Tf 8.5975 0 TD (t)Tj /F4 1 Tf .2822 0 TD (-test)Tj -8.8797 -1.0077 TD [(between)-339.7(PM)-330.9(and)-332.4(OM)]TJ 0 -1.0135 TD (group,*)Tj /F5 1 Tf 3.0866 0 TD (p)Tj /F6 1 Tf .6622 0 TD (<)Tj /F4 1 Tf .7198 0 TD [(0.05,)-332.2(**)]TJ /F5 1 Tf 3.3342 0 TD (p)Tj /F6 1 Tf .668 0 TD (<)Tj /F4 1 Tf .7141 0 TD (0.01;)Tj -9.1849 -1.0135 TD [()50.8(&)47.7()-271.6(represent)]TJ /F5 1 Tf 6.2365 0 TD (t)Tj /F4 1 Tf .2822 0 TD [(-test)-277(between)]TJ -6.5187 -1.0135 TD [(two)-332.2(PM)-336.6(groups,)]TJ 7.3837 0 0 6.5625 501.1085 523.3322 Tm (&)Tj /F5 1 Tf 9.845 0 0 9.845 506.8912 518.9101 Tm (p)Tj /F6 1 Tf .668 0 TD (<)Tj /F4 1 Tf .7198 0 TD (0.05,)Tj 7.3837 0 0 6.5625 433.1337 513.3542 Tm (&&)Tj /F5 1 Tf 9.845 0 0 9.845 444.6991 508.9888 Tm (p)Tj /F6 1 Tf .668 0 TD (<)Tj /F4 1 Tf .7198 0 TD 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-1.0137 -1.1174 TD 0 Tc [(R)15(U)15.3(NX)24.4(2)16.2(,)-245.3(OC)24.7(N)15.2(,)-251.1(S)16.2(O)15.3(X)15(8)16.2(,)]TJ /F4 1 Tf 8.8181 0 TD -.0156 Tc (and)Tj /F5 1 Tf 1.6646 0 TD -.0151 Tc [(CO)-15.1(L)10.8(I)1.1(A1)]TJ /F4 1 Tf 3.3003 0 TD -.0153 Tc [(,)-260.6(h).9(av)-15.3(e)-254.8(a)-.3(lway)-15.3(s)-253.4(b).9(een)-264.1(co)-15.3(n)11.3(s)1.1(id)-15.3(-)]TJ -13.783 -1.1116 TD -.0157 Tc [(er)-15.7(e)9.8(d)-437.3(an)-443(impor)-15.7(t)10.8(a)-.7(nt)-443.1(fact)-15.7(o)10.8(r)-437.2(i)-15.7(n)-427(ear)-15.7(l)10.8(y)-437.3(o)-15.7(s)11.1(teog)-15.7(e)9.7(n).5(ic)-444.2(diff)-15.7(e)9.8(r).5(en)-15.7(t)10.8(i).4(ati)-15.7(o)10.8(n,)]TJ T* -.0153 Tc [(wh)-15.3(i)11.2(l).8(e)]TJ /F5 1 Tf 2.4997 0 TD (SOX9)Tj /F4 1 Tf 2.6149 0 TD 0 Tc [(an)25.4(d)]TJ /F5 1 Tf 1.7509 0 TD -.0153 Tc (OCN)Tj /F4 1 Tf 2.35 0 TD 0 Tc [(a)15(r)16.2(e)-330.6(m)15(ar)25.5(k)16.2(e)15(r)-329.4(p)0(r)26.6(o)16.2(t)16.1(e)15(in)26.5(s)-329.2(i)16.1(n)-329.4(l)16.1(at)25.3(e)-330.6(o)16.2(s)16.4(t)0(e)25.3(o)16.2(g)16.2(e)15(ni)26.5(c)]TJ -9.2155 -1.1116 TD -.0159 Tc [(di)-15.9(f)10.6(f).3(er)-15.9(e)9.6(n).3(tia)-15.9(t)9.4(i).2(on)-15.9(.)]TJ 7.3823 0 0 6.5614 118.7149 685.7006 Tm -.0173 Tc [(41,4)6.6(2)]TJ 9.843 0 0 9.843 139.9181 681.3352 Tm -.0157 Tc [(CoLI)-535.1(ar)-15.7(e)-525.9(i).4(mpor)-15.7(t)10.8(a)-.7(nt)-535.3(co)-15.7(m)9.7(pone)-15.7(n)9.7(t).4(s)-535(o).5(f)-535.1(t)-15.7(h)10.8(e)]TJ -7.9138 -1.1174 TD -.0159 Tc [(ECM)-15.9(.)-292(The)-306.1(e)-.9(xpr)-15.9(e)9.6(s).5(s)-15.9(i)10.8(on)-305(of)]TJ /F5 1 Tf 10.0219 0 TD -.0158 Tc [(COLI)-15.8(A)9.6(1)]TJ /F4 1 Tf 3.5941 0 TD -.0159 Tc [(i)-15.9(n)10.6(crea)-15.9(s)9.7(e)-.9(d,)-307.3(indi)-15.9(c)9.4(a)-.9(tin)-15.9(g)-288.8(a)-15.9(n)-290(i).2(n)-15.9(-)]TJ -13.6159 -1.1116 TD -.0156 Tc [(cr)-15.6(e)9.9(a)-.6(se)-363.4(in)-368(the)-363.4(s).8(y)-15.6(n)11(thes)-15.6(i)11.1(s)-362.1(of)-362.2(n)-15.6(o)11(nmin)-15.6(e)9.8(r).6(al)-15.6(i)10.7(zed)-362.3(EC)-15.6(M)9.1(,)-358.9(w)-15.6(h)10.1(ich)-362.3(i).5(s)-367.8(a)-.6(n)]TJ T* 0 Tc [(i)16.1(m)15(po)26.6(r)16.2(t)16.1(an)25.4(t)-295(s)16.4(te)25.3(p)-294.8(i)16.1(n)-294.8(b)0(o)26.6(n)16.2(e)-296(f)16.2(or)26.6(m)15(a)15(t)16.1(io)26.5(n)16.2(.)]TJ 7.3823 0 0 6.5614 192.6425 652.8187 Tm -.0179 Tc [(9,1)6(1)]TJ 9.843 0 0 9.843 208.0629 648.4534 Tm 0 Tc [(T)15(h)16.2(e)-296(R)0(T)24.4(-)16.2(P)16.3(C)15.2(R)-295.9(r)0(e)25.5(s)16.4(u)16.2(l)0(t)26.3(s)-294.6(in)26.5(-)]TJ -14.837 -1.1174 TD -.016 Tc [(di)-16(c)9.3(a)-1(te)-461.8(th)-16(a)9.4(t)-460.7(the)-461.8(e)-1(x)-16(p)10.6(res)-16(s)11(ion)-460.6(o)-16(f)-450.1(t).1(he)-461.8(os)-16(t)10.7(e)-1(og)-16(e)9.4(n).2(ic)-461.8(di)-16(f)10.5(f).2(er)-16(e)9.5(n).2(ti)-16(a)9.3(tion)-16(-)]TJ 0 -1.1116 TD -.0156 Tc [(re)-15.6(l)9.7(a)-.6(ted)-356.5(g)-15.6(e)9.8(n).6(es)]TJ /F5 1 Tf 5.5524 0 TD 0 Tc [(R)15(U)15.3(NX)24.4(2)16.2(,)-337.5(OC)24.7(N)15.2(,)-343.3(S)16.2(O)15.3(X)15(8)16.2(,)]TJ /F4 1 Tf 9.0946 0 TD -.0156 Tc (and)Tj /F5 1 Tf 1.7567 0 TD -.0151 Tc [(CO)-15.1(L)10.8(I)1.1(A1)]TJ /F4 1 Tf 3.6517 0 TD 0 Tc [(i)16.1(n)-340.9(th)26.5(e)-342.1(O)15.3(M)]TJ -20.0553 -1.1116 TD -.016 Tc [(gr)-16(o)10.6(ups)-224.2(wer)-16(e)-209.4(s)-16(i)10.7(gni)-16()10.6(cant)-16(l)10.3(y)-218.7(h)-16(i)10.5(ghe)-16(r)-209.4(t)-16(h)10.5(a)-1(n)-224.4(t).1(hat)-224.6(i).1(n)-224.4(a)-1(ll)-224.6(the)-225.6(P).3(M)-225.3(grou)-16(p)10.6(s).4(.)]TJ 0 -1.1174 TD -.0156 Tc (The)Tj /F5 1 Tf 1.9122 0 TD -.0151 Tc [(RANKL)-15.1(/)11.5(O).2(PG)]TJ /F4 1 Tf 5.7079 0 TD 0 Tc [(b)16.2(a)15(l)16.1(a)15(nc)25.4(e)-376.6(r)16.2(eg)25.4(u)16.2(l)16.1(at)25.3(e)15(d)-375.5(by)-365.1(o)16.2(s)0(t)26.7(e)15(o)16.2(c)15(la)25.3(s)16.4(t)16.1(/o)26.5(s)16.4(t)16.1(eo)25.4(b)16.2(l)16.1(a)15(s)0(t)]TJ -7.6201 -1.1116 TD [(i)16.1(n)0(t)26.5(e)15(r)16.2(a)15(ct)25.3(i)16.1(o)16.2(ns)-220.9(pl)26.5(a)15(y)16.2(s)-237(a)-238.4(k)16.2(e)15(y)-237.2(r)16.2(o)16.2(l)16.1(e)-238.4(i)16.1(n)-237.2(b)16.2(o)16.2(n)0(e)-222.2(r)0(e)25.5(g)16.2(e)15(n)16.2(er)25.5(a)15(t)16.1(io)26.5(n)16.2(.)-233.8(T)15(h)0(e)-222.2(r)0(e)25.5(l)16.1(a)15(t)16.1(iv)26.5(e)]TJ T* -.0158 Tc [(exp)-15.8(r)10.8(es)-15.8(s)11.2(i).3(on)-345.2(ratio)-345.2(of)]TJ /F5 1 Tf 7.7238 0 TD -.015 Tc [(RU)-15(N)9.7(X)0(2)]TJ /F4 1 Tf 3.3809 0 TD -.0159 Tc [(in)-345.3(the)-340.7(n)-15.9(H)9.8(A)-.6(/Co)-15.9(l)-329.3(gro)-15.9(u)10.7(p)-339.5(was)-345.1(l).2(ow)-15.9(e)8.7(r)]TJ -11.1047 -1.1116 TD [(th)-15.9(a)9.5(n)-178.3(t)-15.9(h)10.6(e)-179.4(r)-15.9(a)9.6(tio)-184(of)-184(CoL)-185.2(a)-.9(nd)-184(DBB/)-15.9(C)9.5(o).3(L)-185.2(i).2(n)-178.3(t)-15.9(h)10.6(e)-179.4(O)-15.9(M)-169.6(gr)-15.9(o)10.7(up.)-186.4(The)]TJ /F5 1 Tf 22.267 0 TD 0 Tc [(OC)24.7(N)]TJ /F4 1 Tf -22.267 -1.1174 TD -.0156 Tc (and)Tj /F5 1 Tf 1.6761 0 TD 0 Tc [(CO)24.7(L)16.3(I)16.2(A)15(1)]TJ /F4 1 Tf 3.571 0 TD -.0159 Tc [(e)-15.9(x)9.5(pres)-15.9(s)11.1(io)-15.9(n)-265.8(i).2(n)-276.2(D)-.6(BB/C)-15.9(o)9.7(L)-277.4(w)-.6(as)-276(si)-15.9(g)10.6(n).3(i)-15.9(c)9.7(a)-.9(ntl)-15.9(y)-265.9(high)-15.9(e)9.5(r)]TJ -5.2471 -1.1116 TD [(th)-16(a)9.4(n)-264.8(i)-16(n)-254.5(the)-271.7(C)-.8(oL)-271.7(gro)-16(u)10.6(p).2(.)-267.1(T)-1(he)-271.7(res)-16(u)10.8(lts)-270.3(in)-270.5(the)-271.7(P).3(M)-265.7(g)-16(r)10.6(oup)-270.5(wer)-16(e)-255.5(con)-16(-)]TJ T* -.0158 Tc [(si)-15.8(s)10.9(t).3(en)-15.8(t)-484.7(w)-.5(ith)-494.9(th)-15.8(e)-485.7(OM.)-491.5(T)-15.8(h)9.6(e)-496.1(o).4(st)-15.8(e)9.5(ogen)-15.8(i)10.7(c)-496.1(d).4(iff)-15.8(e)9.7(ren)-15.8(t)10.7(iati)-15.8(o)10.7(n-r)-15.8(e)9.7(l).3(at)-15.8(e)9.5(d)]TJ 0 -1.1174 TD 0 Tc [(g)16.2(e)15(ne)25.4(s)]TJ /F5 1 Tf 2.4997 0 TD -.0154 Tc (SOX9)Tj /F4 1 Tf 2.5631 0 TD 0 Tc [(an)25.4(d)]TJ /F5 1 Tf 1.6991 0 TD -.0158 Tc [(COLI)-15.8(A)9.6(1)]TJ /F4 1 Tf 3.5883 0 TD -.0159 Tc [(in)-293.5(hBMS)-15.9(C)9.8(s)-293.3(hav)-15.9(e)-278.4(h)-15.9(i)10.6(ghe)-15.9(r)-284.2(e)-.9(xpre)-15.9(s)9.7(s).5(i)-15.9(o)10.6(n)]TJ -10.3502 -1.1116 TD [(in)-224.4(th)-16(e)-209.4(n).2(H)-16(A)8.9(/CoL)-225.6(and)-224.4(D)-.7(B)-16(B)8.5(/CoL)-225.6(gro)-16(u)10.6(p).2(s)-224.2(t).1(ha)-16(n)-209.4(i)-16(n)-208.4(C)-.8(o)-16(L)-209.5(gr)-16(o)10.6(u).2(p)-224.4(a)-1(ft)-16(e)9.3(r)]TJ T* [(7)-258.9(d).3(ay)-15.9(s)-248.3(o).3(f)-258.9(i).2(nc)-15.9(u)9.5(b).3(ati)-15.9(o)10.6(n,)-261.2(espe)-15.9(c)8.4(i).2(all)-15.9(y)-248.6(in)-258.9(the)-260.1(n).3(H)-15.9(A)9(/CoL)-260.1(gr)-15.9(o)10.7(up,)-261.2(whi)-15.9(c)9.4(h)]TJ 0 -1.1174 TD -.0155 Tc [(in)-15.5(d)11.1(i).6(cat)-15.5(e)9.8(d)-275.8(that)-275.9(C)-15.5(a)]TJ 7.3823 0 0 6.5614 127.3889 499.4077 Tm 0 Tc (2)Tj /F6 1 Tf .4761 0 TD (+)Tj /F4 1 Tf 9.843 0 0 9.843 137.537 494.9857 Tm -.0159 Tc [(r)-15.9(e)9.6(lea)-15.9(s)9.7(ed)-276.2(by)-276.2(nH)-15.9(A)-261.7(p)-15.9(r)10.7(o).3(mo)-15.9(t)10.6(e)-.9(d)-276.2(o).3(st)-15.9(e)9.4(obl)-15.9(a)9.4(s).5(t)-276.3(pro)-15.9(-)]TJ -7.6719 -1.1116 TD -.0158 Tc [(li)-15.8(f)10.7(e)-.8(rat)-15.8(i)10.5(on)-264.6(and)-258.8(t)-15.8(h)10.7(e)-260(ex)-15.8(p)10.8(r).4(es)-15.8(s)11.2(i).3(on)-264.6(of)-258.8(o)-15.8(s)11(teo)-15.8(g)10.8(enic)-265.7(gene)-15.8(s)9.8(.)]TJ 7.3823 0 0 6.5614 251.0928 488.4093 Tm 0 Tc (9)Tj 9.843 0 0 9.843 72 473.1022 Tm [(Fr)21(om)-452.7(th)15(e)-463(a)0(b)19.7(o)0(v)20.8(e)0(,)-454.9(t)0(h)20.7(e)-468.8(DB)18.9(B)-462.9(w)0(e)18.8(r)0(e)-452.6(o)0(r)15.1(i)0(g)20.7(i)0(n)20.7(a)0(l)19.5(l)0(y)-457.3(f)0(r)20.9(o)0(m)-452.7(n)0(a)19.7(t)0(u)15(r)0(a)19.7(l)]TJ -1.0137 -1.1174 TD [(bo)20.8(ne)19.7(,)-521.8(a)0(n)19.7(d)-513.7(th)15(e)-514.9(m)0(i)19.5(c)0(r)19.7(o)0(-)-509(a)0(n)19.7(d)-519.5(ma)18.4(cr)19.7(op)20.8(or)20.9(ou)15.1(s)-513.5(s)0(t)15.1(r)0(u)20.9(c)0(t)19.5(u)0(r)20.9(e)0(s)-510(w)0(e)18.8(r)0(e)-510.2(a)]TJ 0 -1.1116 TD [(be)19.7(ne)19.7(t)-232.6(to)-227(va)19.7(sc)19.9(ul)20.7(ar)14(iz)19.6(at)19.5(io)20.7(n)-237.2(d)0(u)15.1(r)0(i)20.7(n)0(g)-226.8(b)0(o)20.8(n)0(e)-228(d)0(e)13.9(f)0(e)19.7(c)0(t)-228.1(r)0(e)19.7(p)0(a)19.7(i)0(r)20.7(.)-239.6(To)-228(ob)15.1(ta)19.6(in)]TJ T* [(th)20.7(e)-428.5(b)0(e)19.7(s)0(t)-416.8(a)0(p)19.7(p)0(l)20.7(i)0(c)13.8(a)0(b)19.7(l)0(e)-412.4(r)0(a)14(t)0(i)20.6(o)0(,)-413.5(a)0(n)19.7(d)-427.3(ex)19.7(pl)15(or)20.9(e)-422.7(t)0(h)15(e)-422.7(mo)19.6(st)-416.8(bi)15(om)19.6(im)19.5(et)19.5(ic)]TJ T* [(na)19.7(tu)20.7(ra)14(l)-358.3(b)0(o)15.1(n)0(e)-354.7(f)0(o)20.9(r)-363.9(cl)19.5(in)20.7(ic)19.6(al)-354.8(ap)19.7(pl)20.7(ic)13.8(at)19.5(io)20.7(n)-364(o)0(f)-353.5(t)0(h)20.7(e)-365.1(pr)20.9(in)20.7(ti)20.6(ng)-353.5(pr)15.1(oc)19.7(es)19.9(s,)]TJ 0 -1.1174 TD [(th)20.7(e)-313.3(d)0(i)20.7(f)0()21(c)0(u)19.7(l)0(t)20.6(i)0(e)13.8(s)-306.2(of)-295.9(th)15(e)-307.5(p)0(a)19.7(r)0(t)20.7(i)0(c)13.8(l)0(e)-297.2(s)0(i)20.9(z)0(e)-304(a)0(n)19.7(d)-306.4(ir)20.7(re)19.7(gu)15.1(la)19.6(r)-306.3(s)0(h)21(a)0(p)13.9(e)-307.5(of)-295.9(th)15(is)]TJ 0 -1.1116 TD [(tr)20.7(ab)19.7(ec)12.8(ul)20.7(ar)-314.4(bo)20.8(ne)-314.4(gr)20.9(af)19.7(t)-323.8(w)0(e)18.8(r)0(e)-314.4(o)0(v)15.1(e)0(r)19.7(c)0(o)19.7(m)0(e)-315.6(i)0(n)-313.3(t)0(h)20.7(i)0(s)-313.2(s)0(t)20.9(u)0(d)20.8(y)0(.)]TJ /F8 1 Tf 8.9663 0 0 8.9663 62.022 384.4345 Tm (Conclusion)Tj /F4 1 Tf 9.843 0 0 9.843 72 367.9936 Tm [(Both)-449.1(two)-447.8(composite)-457(bioinks,)-448.9(nHA/CoL)-449.6(and)-453.7(DBB/CoL,)]TJ -1.0137 -1.1174 TD [(were)-307.1(used)-304.9(to)-307.6(print)-305.2(biomimic)-306.6(3D)-302.6(scaffolds)-311.4(successfully.)-307.8(The)]TJ 0 -1.1116 TD [(two)-205.9(kinds)-201.3(of)-203.8(hydroxyapatite)-209.3(composite)-209.4(which)-203.5(favor)-208.2(hBMSCs)]TJ T* [(proliferation)-587.3(and)-586.2(differentiation)-585(proved)-589.5(to)-578.3(be)-585.1(promising)]TJ T* [(candidates)-378.6(for)-371.9(a)-376.6(3)0(D)-377.4(scaffold)-373.9(bioink.)-373.2(A)-376.3(porous)-375(architecture)]TJ 0 -1.1174 TD [(was)-270.1(manufactured)-272.6(eventually.)-271.8(Further)-268(studies)-273.7(would)-265.7(need)-271.7(to)]TJ 0 -1.1116 TD [(be)-383.5(concentrated)-387.9(on)-376.6(the)-384.7(DBB)-380.6(as)-383.3(a)-382.4(bioink)-381.3(to)-382.5(build)-380.1(up)-382.3(a)-382.4(3)0(D)]TJ T* [(printing)-262.6(customized)-263.5(bone)-264.8(substitute)-260(in)-261.5(clinical)-266.2(application.)]TJ /F8 1 Tf 8.9663 0 0 8.9663 62.022 270.3684 Tm [(Authors)-334(Contributions)]TJ /F4 1 Tf 9.843 0 0 9.843 72 253.9275 Tm [(Qin)13(g)-162.4(L)0(i)-159(and)-148.5(Xion)11.9(gxi)13.8(n)-162.4(Lei)-155.5(equ)12.8(ally)-149.8(cont)11.6(rib)13.9(uted)-149.7(to)-157.8(this)-148.5(arti)11.5(cle)11.5(.)]TJ -1.0137 -1.1174 TD [(Peij)11.6(un)-249.9(Lyu)-252.2(and)-246.4(Gui)13(feng)-247.4(Zha)11.6(ng)-255.6(are)-247.5(corr)11.8(esp)13(ondi)12.7(ng)-249.9(auth)11.6(ors)14.2(.)]TJ /F8 1 Tf 8.9663 0 0 8.9663 62.022 220.0252 Tm (Acknowledgment)Tj /F4 1 Tf 9.843 0 0 9.843 72 203.5842 Tm [(This)-362.6(work)-368.1(was)-368(supported)-362.3(by)-365.1(the)-367.5(National)-363.7(Key)-368.2(Research)]TJ -1.0137 -1.1116 TD [(and)-332.8(Development)-340.5(Program)-332.4(of)-336.2(China)-336.1(\(2016YFC1102900\).)]TJ /F8 1 Tf 8.9663 0 0 8.9663 62.022 171.7228 Tm [(Disclosure)-336.7(Statement)]TJ /F4 1 Tf 9.843 0 0 9.843 72 155.2818 Tm [(No)-331.4(competing)-336.3(nancial)-340.7(interests)-334.7(exist.)]TJ /F8 1 Tf 8.9663 0 0 8.9663 62.022 134.3622 Tm (References)Tj /F4 1 Tf 9.2951 0 0 9.2951 66.6708 118.3181 Tm [(1.)-500.4(Provaggi,)-406.5(E.,)-398.6(Leong,)-403.4(J.J.H.,)-409.2(and)-401.1(Kalaskar,)-406.6(D.M.)-403.8(Applica-)]TJ 1.2503 -1.1284 TD [(tions)-431.7(of)-429.6(3D)-428.8(printing)-428.7(in)-426.7(the)-431(management)-431.6(of)-429.6(severe)-435.8(spinal)]TJ 0 -1.1223 TD [(conditions.)-332.5(Proc)-339.4(Inst)-330.8(Mech)-337.6(Eng)-331.7(H)]TJ /F7 1 Tf 14.3759 0 TD (6,)Tj /F4 1 Tf 1.0796 0 TD [(231,)-336(2017.)]TJ -16.7058 -1.1284 TD [(2.)-500.4(Noshin,)-507.3(M.,)-499.7(Baker,)-510.4(H.B.,)-507(Taskoy,)-506.3(E.,)]TJ /F5 1 Tf 17.8891 0 TD [(et)-504.1(al.)]TJ /F4 1 Tf 2.7691 0 TD [(3D)-502(printed)]TJ -19.4078 -1.1284 TD [(biofunctionalized)-708(scaffolds)-707.3(for)-706.5(microfracture)-703.4(repair)-708.7(of)]TJ 0 -1.1223 TD [(cartilage)-335.9(defects.)-337.8(Biomaterials)]TJ /F7 1 Tf 12.6986 0 TD (185,)Tj /F4 1 Tf 2.0798 0 TD [(219,)-336(2018.)]TJ 10.9786 70.9158 TD [(3.)-500.4(Bruyas,)-432.2(A.,)-428.8(Lou,)-429.4(F.,)-430.1(Stahl,)-433.7(A.M.,)]TJ /F5 1 Tf 15.7116 0 TD [(et)-430.9(al.)]TJ /F4 1 Tf 2.6166 0 TD [(Systematic)-434.8(char-)]TJ -17.0779 -1.1223 TD [(acterization)-480.2(of)-466.1(3D-printed)-474.3(PCL/)]TJ /F10 1 Tf 13.4549 0 TD (b)Tj /F4 1 Tf .5489 0 TD [(-TCP)-469.9(scaffolds)-475.6(for)-468.6(bio-)]TJ -14.0038 -1.1284 TD [(medical)-371.9(devices)-372(and)-364.5(bone)-370.7(tissue)-368.1(engineering:)-368.7(inuence)-369.9(of)]TJ 0 -1.1223 TD [(composition)-338.2(and)-334(porosity.)-337(J)-328.6(Mater)-339.5(Res)]TJ /F7 1 Tf 16.2179 0 TD (14,)Tj /F4 1 Tf 1.5858 0 TD [(33,)-335.8(2018.)]TJ -19.054 -1.1283 TD -.0188 Tc [(4)-18.8(.)-519.2(Boga,)-390.9(J).2(.C.,)-390.9(Mi)6(guel,)-390.9(S.P.,)-390.9(d)-.6(e)-389.1(M).1(elo-Dio)5.5(go,)-390.9(D.)5.5(,)]TJ /F5 1 Tf 19.7737 0 TD -.0161 Tc [(et)-387.7(al)8.7(.)]TJ /F4 1 Tf 2.4519 0 TD -.0202 Tc [(In)-392.4(vitro)]TJ -20.9753 -1.1223 TD -.0194 Tc [(c)-5.4(h)4.9(aracteriza)-5.4(t)5.4(ion)-330.6(o)-1.2(f)-332.9(3)4.9(D)-338.3(p)4.9(rinted)-330.6(sca)-5.4(ffolds)-329.7(a).7(imed)-330.6(at)-336.1(bone)-328.7(tissue)]TJ 0 -1.1284 TD -.0207 Tc [(regene)-6.7(ration.)-173.2(Colloids)]TJ 8.5877 0 TD -.0197 Tc [(Surfaces)-171.4(B)-171(B)-.2(iointerfaces)]TJ /F7 1 Tf 9.5392 0 TD -.0182 Tc [(16)6.1(5,)]TJ /F4 1 Tf 1.842 0 TD [(2)6.1(07,)-164.7(201)6.1(8.)]TJ -21.2192 -1.1223 TD 0 Tc [(5.)-500.4(Berzina-Cimdina,)-567.9(L.,)-557.2(and)-553.6(Borodajenko,)-559.5(N.)-556.8(Research)-560.9(of)]TJ 1.2503 -1.1284 TD [(calcium)-347.5(phosphates)-343(using)-334.7(Fourier)-341.5(transform)-346(infrared)-339.2(spec-)]TJ 0 -1.1223 TD [(troscopy.)-415(In:)-404.7(Theophanides)-417.4(T,)-404.7(ed.)-413.2(Materials)-409.3(Science,)-416.7(En-)]TJ 0 -1.1284 TD [(gineering)-340.2(and)-334(Technology,)-334.5(2015,)-330(pp.)-335.8(123148.)]TJ -1.2503 -1.1223 TD [(6.)-500.4(Camacho,)-445(N.P.,)-438(West,)-434.7(P.,)-442.3(Torzilli,)-434.3(P.A.,)]TJ /F5 1 Tf 18.5905 0 TD [(et)-443.1(al.)]TJ /F4 1 Tf 2.6349 0 TD [(FTIR)-439.4(mi-)]TJ -19.975 -1.1284 TD [(croscopic)-350.2(imaging)-345.2(of)-344.2(collagen)-342(and)-346.2(proteoglycan)-344.9(in)-347.4(bovine)]TJ T* [(cartilage.)-342.1(Biopolymers)]TJ /F7 1 Tf 9.5636 0 TD (1,)Tj /F4 1 Tf 1.0857 0 TD [(62,)-329.7(2001.)]TJ -11.8996 -1.1223 TD [(7.)-500.4(Lin,)-270.2(K.F.,)-273.3(He,)-268.4(S.,)-265.4(Song,)-271.9(Y.,)]TJ /F5 1 Tf 12.5156 0 TD [(et)-272.3(al.)]TJ /F4 1 Tf 2.2994 0 TD [(Low)-272.3(temperature)-271.6(additive)]TJ -13.5647 -1.1284 TD [(manufacturing)-243.2(biomimic)-240.7(three-dimensional)-246(hydroxyapatite/)]TJ 0 -1.1223 TD [(collagen)-323.7(scaffolds)-323.1(for)-322.2(bone)-315.8(regeneration.)-327.1(ACS)-320.7(Appl)-318.7(Mater)]TJ 0 -1.1284 TD (Interfaces)Tj /F7 1 Tf 4.2939 0 TD (11,)Tj /F4 1 Tf 1.5858 0 TD [(8,)-329.6(2016.)]TJ -7.13 -1.1223 TD [(8.)-500.4(Inzana,)-320.8(J.A.,)-318.4(Olvera,)-323.7(D.,)-319(Fuller,)-314.2(S.M.,)]TJ /F5 1 Tf 16.9681 0 TD [(et)-321.1(al.)]TJ /F4 1 Tf 2.397 0 TD [(3D)-312.9(printing)-318.9(of)]TJ -18.1147 -1.1284 TD [(composite)-501.3(calcium)-493.9(phosphate)-496.2(and)-498.7(collagen)-494.5(scaffolds)-500(for)]TJ 0 -1.1223 TD [(bone)-334.1(regeneration.)-339.3(Biomaterials)]TJ /F7 1 Tf 13.3573 0 TD (13,)Tj /F4 1 Tf 1.5797 0 TD [(35,)-335.8(2014.)]TJ -16.1874 -1.1284 TD [(9.)-500.4(Wahl,)-307.5(D.A.,)-302.5(and)-297.4(Czernuszka,)-311.2(J.T.)-300.3(Collagen-hydroxyapatite)]TJ 1.2503 -1.1223 TD [(composites)-195.6(for)-181.9(hard)-190.1(tissue)-191.2(repair.)-190.3(Eur)-187.6(Cell)-185.3(Mater)]TJ /F7 1 Tf 19.3529 0 TD (1,)Tj /F4 1 Tf .9393 0 TD [(11,)-183.3(2006.)]TJ -22.0426 -1.1284 TD [(10.)-500.5(Zhou,)-423.4(H.,)-422.7(and)-419.4(Lee,)-425.6(J.)-420.2(Nanoscale)-420.8(hydroxyapatite)-430(particles)]TJ 1.7505 -1.1223 TD [(for)-334.4(bone)-334.1(tissue)-337.6(engineering.)-338.7(Acta)-333.3(Biomater)]TJ /F7 1 Tf 18.0232 0 TD (7,)Tj /F4 1 Tf 1.0796 0 TD [(7,)-335.7(2011.)]TJ -20.8533 -1.1284 TD [(11.)-500.5(Li,)-263.9(Q.,)-264.1(Wang,)-259.3(T.,)-264.4(Zhang,)-263.1(G.F.,)]TJ /F5 1 Tf 14.2783 0 TD [(et)-260.1(al.)]TJ /F4 1 Tf 2.2811 0 TD [(A)-264(comparative)-263.1(evalua-)]TJ -14.8089 -1.1223 TD [(tion)-237.3(of)-240.5(the)-235.8(mechanical)-240.5(properties)-238.1(of)-240.5(two)-239.3(calcium)-237.7(phosphate/)]TJ 0 -1.1284 TD [(collagen)-220(composite)-214.6(materials)-220.6(and)-212(their)-213.5(osteogenic)-219.4(effects)-218.1(on)]TJ 0 -1.1223 TD [(adipose-derived)-265.6(stem)-265.2(cells.)-263.3(Stem)-256.7(Cells)-263.8(Int)]TJ /F7 1 Tf 17.1998 0 TD (395,)Tj /F4 1 Tf 2.0067 0 TD [(2016,)-262.9(2016.)]TJ -20.957 -1.1284 TD [(12.)-500.5(Va)]TJ 2.5373 .0427 TD ()Tj .3842 -.0427 TD [(zquez,)-501.4(C.G.,)-500.9(Barba,)-504.3(C.P.,)-502.2(and)-498.7(Mungu)]TJ 16.1569 .0427 TD ()Tj .305 -.0427 TD [(a,)-498.5(N.)-501.9(Stoichio-)]TJ -17.6329 -1.1223 TD [(metric)-290.6(hydroxyapatite)-289.7(obtained)-289(by)-280.8(precipitation)-288.4(and)-285.2(sol)-291.7(gel)]TJ 0 -1.1284 TD [(processes.)-336.9(Rev)-338.8(Mex)-333.3(Fis)]TJ /F7 1 Tf 10.082 0 TD (3,)Tj /F4 1 Tf 1.0857 0 TD [(51,)-335.8(2005.)]TJ -12.9182 -1.1223 TD [(13.)-500.5(Kobayashi,)-375.9(K.,)-367.8(Anada,)-370.6(T.,)-368.1(Handa,)-370.6(T.,)]TJ /F5 1 Tf 17.6695 0 TD [(et)-369.9(al.)]TJ /F4 1 Tf 2.4946 0 TD (Osteoconduc-)Tj -18.4136 -1.1284 TD [(tive)-631.9(property)-625.5(of)-624.7(a)-626.5(mechanical)-630.8(mixture)-628.1(of)-624.7(octacalcium)]TJ 0 -1.1223 TD [(phosphate)-435.2(and)-431.6(amorphous)-427.4(calcium)-432.9(phosphate.)-435.3(ACS)-430.5(Appl)]TJ 0 -1.1284 TD [(Mater)-339.5(Interfaces)]TJ /F7 1 Tf 7.0202 0 TD (24,)Tj /F4 1 Tf 1.5858 0 TD [(6,)-335.7(2014.)]TJ -10.3565 -1.1223 TD [(14.)-500.5(Li,)-337.1(L.,)-337.6(Zhao,)-336.1(M.,)-335(Li,)-337.1(J.,)]TJ /F5 1 Tf 11.7532 0 TD [(et)-333.3(al.)]TJ /F4 1 Tf 2.4275 0 TD [(Preparation)-337.8(and)-334(cell)-337.2(inltra-)]TJ -12.4302 -1.1284 TD [(tion)-261.7(of)-252.7(lotus-type)-261.5(porous)-258.5(nano-hydroxyapatite/polyurethane)]TJ 0 -1.1223 TD [(scaffold)-262.8(for)-255.1(bone)-261(tissue)-258.3(regeneration.)-260(Mater)-260.2(Lett)]TJ /F7 1 Tf 19.7188 0 TD (149,)Tj /F4 1 Tf 2.0066 0 TD (2015.)Tj -23.476 -1.1284 TD [(15.)-500.5(Krishnan,)-532.4(V.,)-526.4(Bhatia,)-531.4(A.,)-532.5(and)-523.1(Varma,)-531.1(H.)-532.4(Development,)]TJ 1.7505 -1.1223 TD [(characterization)-479.4(and)-462.1(comparison)-473.8(of)-466.1(two)-465(strontium)-470.2(doped)]TJ 0 -1.1284 TD [(nano)-382.9(hydroxyapatite)-381.2(molecules)-383.5(for)-377.1(enamel)-378.4(repair/regener-)]TJ 0 -1.1223 TD [(ation.)-339.3(Dent)-335.1(Mater)]TJ /F7 1 Tf 7.6057 0 TD (5,)Tj /F4 1 Tf 1.0857 0 TD [(32,)-329.7(2016.)]TJ 9.2951 0 0 9.2951 313.0582 275.3574 Tm [(16.)-500.5(Ki)17(ku)18(ch)19.9(i)12.6(,)-164.7(M.)18.8(,)-164.7(I)0(t)22.4(o)0(h)18(,)-158.6(S.)15.2(,)-158.6(I)0(c)17.6(h)0(i)18.6(n)0(o)18(s)12.9(e,)-156.9(S.)21.3(,)-164.7(S)0(h)21.2(i)12.6(no)18(m)12.5(i)0(y)18.6(a)0(,)-156.9(K)0(.)22.6(,)-164.7(an)19.9(d)-164.8(T)0(a)18(n)0(a)19.9(k)0(a)19.9(,)]TJ 1.7505 -1.1223 TD [(J.)-151.8(Se)17(l)12.6(f)0(-)19.5(o)0(r)15.7(g)0(a)19.9(n)0(i)24.7(z)0(a)15.7(t)0(i)19.2(o)0(n)-146.7(m)0(e)14.2(c)14(ha)13.8(ni)24.7(sm)-151.4(in)-152.2(a)-156.8(b)0(o)18(n)0(e)19.9(-)0(l)22.4(i)0(k)18.6(e)-162.9(hy)24.1(dr)15.7(ox)24.1(ya)13.8(pa)19.9(t)12.6(i)0(t)19.2(e)0(/)]TJ 0 -1.1284 TD [(co)13.8(l)12.6(l)12.6(a)0(g)13.8(e)14(n)-170.9(n)0(a)19.9(n)0(o)18(c)0(o)19.9(m)0(p)18.4(o)0(s)18.9(i)12.6(te)-162.5(sy)18.9(nt)18.6(he)19.9(si)19.5(ze)15.7(d)-170.9(i)12.6(n)-170.9(v)0(i)18.6(t)12.6(ro)-161.2(an)13.8(d)-164.8(i)0(t)19.2(s)-176.1(bi)24.7(ol)18.6(og)18(i)12.6(c)0(a)15.7(l)]TJ T* [(re)17.6(ac)15.7(t)12.6(i)0(o)18.6(n)-250.2(in)-243.7(vi)24.7(vo)18(.)-250.1(B)0(i)19.9(o)0(m)18.5(a)0(t)20.5(e)0(r)17.6(i)12.6(al)14.4(s)]TJ /F7 1 Tf 11.9606 0 TD [(13)18(,)]TJ /F4 1 Tf 1.4821 0 TD -.018 Tc [(2)-18(2)6.1(,)-268.1(2)-18(00)-18(1.)]TJ -15.1932 -1.1223 TD 0 Tc [(17.)-500.5(Lu)28.3(d)18.2(w)16.6(i)12.6(g)18.2(,)-323.3(S)21.4(.C)31.6(.,)-305.1(K)16.6(o)0(w)28.6(a)14(l)18.7(s)12.9(k)18.2(i)18.7(,)-323.3(J)19(.M)31(.,)-305.1(a)14(n)18.2(d)-317.3(B)13.4(o)18.2(d)18.2(e)14(n,)-305.2(S)15.3(.)18.2(D)16.6(.)-323.3(O)16.6(s)19(t)12.6(e)13.9(o)18.2(i)18.7(nd)30.2(uc)32.1(-)]TJ 1.7505 -1.1284 TD -.0161 Tc [(tiv)-16.1(e)-459.7(bo)-16.1(n)14.1(e)-471.8(g)-16.1(r)11.8(aft)-473.1(s)2.9(u)-16.1(b)14.1(stitutes.)-473.6(E).2(u)-16.1(r)11.8(opean)-473.7(spin)-16.1(e)-459.7(j)-3.5(ourn)-16.1(a)16(l)-473.1(o)-16.1(f)11.8()-.8(cial)]TJ 0 -1.1223 TD -.0166 Tc [(p)-16.6(u)13.6(bl)-4(icat)-4(ion)-388.8(o)-16.6(f)-373(t)-4(he)-393(Eur)-391.1(S)-1.3(pin)-16.6(e)-374.8(J)-3.7(,)-388.7(th)-16.6(e)-374.8(E)-.3(u)-16.6(r)11.3(opean)-388.8(Spi)-4(n)1.6(al)-388.2(Defor-)]TJ 0 -1.1284 TD -.0157 Tc [(mity)-265.9(Society,)-265.8(and)-265.9(t)3(h)-15.7(e)-252(Eur)-15.7(o)12.2(pean)-265.9(Se)4.4(ctio)-15.7(n)-247.8(o)-15.7(f)-250.1(t)-3.1(he)-270.1(C)3.8(e)-1.7(rvica)4.4(l)-265.3(Sp)-15.7(i)15.1(n)-15.7(e)]TJ 0 -1.1223 TD -.0151 Tc [(Research)-332.4(Society)]TJ /F7 1 Tf 7.1178 0 TD -.0182 Tc (9,)Tj /F4 1 Tf 1.0491 0 TD [(1:)-5.6(20)-18.2(0)12(0)0(.)]TJ -9.9174 -1.1284 TD .0114 Tc [(18.)-494.9(Boh)5.2(n)-.9(er,)-415.6(M)-.2(.)-415.6(C).4(alci)11.4(u)-6.6(m)-415.2(orth)5.2(op)5.2(hos)11.4(p)-6.3(h)-.9(at)11.4(e)-10.8(s)-414.8(i)11.4(n)-421.4(medi)11.4(cine:)-415.1(from)]TJ 1.781 -1.1223 TD .0108 Tc [(cerami)10.8(c)-5.3(s)-561.8(t)-1(o)-556.6(calci)10.8(u)-7.2(m)-556.1(phosphate)-560.8(cements.)-556.5(I)2.2(njury)]TJ /F7 1 Tf 21.3473 0 TD .0123 Tc [(10)6.1(,)]TJ /F4 1 Tf 1.8359 0 TD (4,)Tj -23.1832 -1.1284 TD [(200)6.1(0.)]TJ -1.781 -1.1223 TD 0 Tc [(19.)-500.5(Wang,)-350.8(T.,)-343.7(Li,)-349.3(Q.,)-343.4(Zhang,)-348.5(G.F.,)]TJ /F5 1 Tf 14.7845 0 TD [(et)-345.5(al.)]TJ /F4 1 Tf 2.4519 0 TD [(Comparative)-349.1(evalua-)]TJ -15.486 -1.1284 TD [(tion)-584.9(of)-575.9(a)-583.8(biomimic)-582.2(collagen/hydroxyapatite/)]TJ /F10 1 Tf 19.0052 0 TD (b)Tj /F4 1 Tf .555 0 TD (-tricaleium)Tj -19.5602 -1.1223 TD [(phosphate)-301(scaffold)-299.4(in)-298.6(alveolar)-301.6(ridge)-299.4(preservation)-297.5(with)-299.9(Bio-)]TJ 0 -1.1284 TD [(Oss)-335.7(Collagen.)-336.5(Front)-334.7(Mater)-333.4(Sci)]TJ /F7 1 Tf 12.8755 0 TD (2,)Tj /F4 1 Tf 1.0857 0 TD [(10,)-329.7(2016.)]TJ -15.7116 -1.1223 TD [(20.)-500.5(Liu,)-386.1(C.,)-383.2(Han,)-384.4(Z.,)-380.3(and)-382.8(Czernuszka,)-390.5(J.T.)-379.6(Gradient)-384.4(collagen/)]TJ 1.7505 -1.1284 TD [(nanohydroxyapatite)-440.9(composite)-434.2(scaffold:)-439.3(development)-439.6(and)]TJ 0 -1.1223 TD [(characterization.)-345.3(Acta)-333.3(Biomater)]TJ /F7 1 Tf 13.3146 0 TD (2,)Tj /F4 1 Tf 1.0857 0 TD [(5,)-329.6(2009.)]TJ -16.1508 -1.1284 TD [(21.)-500.5(Bo)19.3(se)14.7(,)-158.6(S)15.3(.,)-152.6(Va)18.3(ha)19.9(bz)19.9(ad)19.9(eh)19.9(,)-158.6(S)0(.)21.3(,)-158.6(an)19.9(d)-158.7(B)13.4(an)13.8(dy)24.1(op)18(ad)19.9(hy)18(ay)19.9(,)-158.6(A)0(.)-148.2(B)13.4(on)18(e)-156.8(t)0(i)19.2(s)0(s)19.8(u)0(e)]TJ 1.7505 -1.1223 TD [(en)13.8(gi)24.7(ne)19.9(er)17.6(in)18.6(g)-250.2(u)0(s)18.9(i)12.6(ng)-244.3(3D)-239.7(pr)21.8(in)18.6(ti)19.2(ng)18(.)-250.1(M)12.8(at)20.5(er)-244.7(To)22.2(da)13.8(y)]TJ /F7 1 Tf 17.7061 0 TD [(12)24.1(,)]TJ /F4 1 Tf 1.476 0 TD [(16)18(,)-250.1(2)0(0)24.1(1)0(3)18(.)]TJ /F8 1 Tf 8.9663 0 0 8.9663 62.022 749.6502 Tm (1270)Tj 50.2102 0 TD [(LI)-331.6(ET)-338.7(AL.)]TJ ET endstream endobj 95 0 obj <>stream /GS1 gs BT /F4 1 Tf 9.2951 0 0 9.2951 59.7543 725.1588 Tm 0 0 0 rg 0 Tc 0 Tw [(22.)-500.5(Wang,)-381.3(X.,)-373.9(Song,)-375.6(Y.,)-373.9(Liu,)-380(Y.,)]TJ /F5 1 Tf 14.2539 0 TD [(et)-376(al.)]TJ /F4 1 Tf 2.5129 0 TD [(Osteogenic)-379.6(differenti-)]TJ -15.0163 -1.1223 TD [(ation)-327(of)-325.9(three-dimensional)-337.5(bioprinted)-323.4(constructs)-332.5(consisting)]TJ 0 -1.1284 TD [(of)-374.7(human)-370.5(adipose-derived)-375.4(stem)-368.8(cells)-373.1(in)-371.8(vitro)-367.8(and)-370.6(in)-371.8(vivo.)]TJ 0 -1.1223 TD [(PLos)-333.8(One)]TJ /F7 1 Tf 4.3853 0 TD (6,)Tj /F4 1 Tf 1.0857 0 TD [(11,)-329.7(2016.)]TJ -7.2215 -1.1283 TD [(23.)-500.5(Wang,)-314.2(X.F.,)-309.9(Lu,)-307.2(P.J.,)-313.6(Song,)-308.5(Y.,)]TJ /F5 1 Tf 14.8516 0 TD [(et)-308.9(al.)]TJ /F4 1 Tf 2.3787 0 TD [(Nano)-311.3(hydroxyapatite)]TJ -15.4799 -1.1223 TD [(particles)-349.1(promote)-348.1(osteogenesis)-346(in)-347.4(a)-345.9(three-dimensional)-349.7(bio-)]TJ 0 -1.1284 TD [(printing)-666.6(construct)-668.7(consisting)-667(of)-667.4(alginate/gelatin/hASCs.)]TJ 0 -1.1223 TD [(RSC)-336.1(Adv)]TJ /F7 1 Tf 4.2695 0 TD (8,)Tj /F4 1 Tf 1.0857 0 TD [(6,)-335.7(2016.)]TJ -7.1056 -1.1284 TD [(24.)-500.5(Klammert,)-267.7(U.,)-258(Gbureck,)-263.5(U.,)-258(Vorndran,)-261.7(E.,)]TJ /F5 1 Tf 18.9686 0 TD [(et)-260.1(al.)]TJ /F4 1 Tf 2.2811 0 TD [(3D)-258(powder)]TJ -19.4992 -1.1223 TD [(printed)-451.6(calcium)-451.2(phosphate)-447.4(implants)-447.6(for)-450.3(reconstruction)-449.7(of)]TJ 0 -1.1284 TD [(cranial)-795.5(and)-797.5(maxillofacial)-798.4(defects.)-795.3(Journal)-792.9(of)-795.5(cranio-)]TJ 0 -1.1223 TD [(maxillofacial)-341(surgery)]TJ /F7 1 Tf 8.9781 0 TD (8,)Tj /F4 1 Tf 1.0857 0 TD [(38,)-335.8(2010.)]TJ -11.8142 -1.1284 TD [(25.)-500.5(Vorndran,)-249.5(E.,)-252.2(Klammert,)-249.4(U.,)-251.9(Ewald,)-251.9(A.,)]TJ /F5 1 Tf 18.011 0 TD [(et)-247.9(al.)]TJ /F4 1 Tf 2.2567 0 TD (Simultaneous)Tj -18.5173 -1.1284 TD [(immobilization)-305(of)-295.4(bioactives)-298.1(during)-301.5(3D)-294.6(powder)-301.7(printing)-294.5(of)]TJ 0 -1.1223 TD [(bioceramic)-293.5(drug-release)-286.3(matrices.)-288.2(Adv)-288.7(Funct)-287.8(Mater)]TJ /F7 1 Tf 21.1887 0 TD (10,)Tj /F4 1 Tf 1.537 0 TD (20,)Tj -22.7257 -1.1284 TD (2010.)Tj -1.7505 -1.1223 TD [(26.)-500.5(Butscher,)-241(A.,)-233.6(Bohner,)-237.9(M.,)-231.3(Hofmann,)-240.5(S.,)]TJ /F5 1 Tf 17.8586 0 TD [(et)-235.7(al.)]TJ /F4 1 Tf 2.2262 0 TD [(Structural)-241.4(and)]TJ -18.3343 -1.1284 TD [(material)-331.1(approaches)-336.9(to)-329.1(bone)-328(tissue)-331.5(engineering)-326.5(in)-329.1(powder-)]TJ 0 -1.1223 TD [(based)-240(three-dimensional)-233.8(printing.)-233.6(Acta)-235.7(Biomater)]TJ /F7 1 Tf 19.7615 0 TD (3,)Tj /F4 1 Tf .982 0 TD [(7,)-232(2011.)]TJ -22.494 -1.1284 TD [(27.)-500.5(Ch)19.3(an)19.9(dr)15.7(an)19.9(,)-506.3(P)0(.)21.3(L)0(.)16.2(,)-500.2(Pa)17(ik)18.6(,)-506.3(D)0(.)22.6(C)0(.)19.4(,)-506.3(an)19.9(d)-506.4(H)0(o)16.4(l)12.6(me)14.2(s)12.9(,)-506.3(J.)19(W.)-498.6(S)15.3(t)0(r)16.3(u)0(c)19.9(t)0(u)18.6(r)0(a)17.6(l)]TJ 1.7505 -1.1223 TD [(me)14.2(c)14(h)0(a)19.9(n)0(i)18.6(s)0(m)-578.4(f)0(o)21.8(r)-588(al)20.5(te)14.4(ra)17.6(t)12.6(i)12.6(o)0(n)-579.7(o)0(f)-575.9(c)0(o)19.9(l)0(l)19.2(a)0(g)19.9(e)0(n)-577.8(g)0(e)19.9(l)-585.1(me)14.2(ch)19.9(an)19.9(i)12.6(c)0(s)-583(b)0(y)]TJ 0 -1.1284 TD [(gl)18.6(ut)18.6(ar)17.6(al)20.5(de)19.9(hy)18(de)-218(cr)17.6(os)18.9(s)12.9(l)0(i)19.2(n)0(k)18(i)12.6(ng)18(.)-231.8(C)13.4(on)18(ne)19.9(ct)-217.4(Ti)16.7(ss)19.8(ue)-218(Re)15.1(s)]TJ /F7 1 Tf 19.3407 0 TD (4,)Tj /F4 1 Tf .9637 0 TD [(53)18(,)-231.8(2)0(0)24.1(1)0(2)18(.)]TJ -22.0548 -1.1223 TD [(28.)-500.5(Perrien,)-370.2(D.S.,)-370.9(Young,)-362.2(C.S.,)-368(Alvarezurena,)-376.2(P.P.,)]TJ /F5 1 Tf 21.5669 0 TD [(et)-363.8(al.)]TJ /F4 1 Tf 2.4946 0 TD (Per-)Tj -22.311 -1.1284 TD [(cutaneous)-738.3(injection)-733.2(of)-734.5(augment)-734.2(injectable)-735.7(bone)-730.6(graft)]TJ 0 -1.1223 TD [(\(rhPDGF-BB)-435.6(and)]TJ /F10 1 Tf 7.685 0 TD (b)Tj /F4 1 Tf .555 0 TD [(-tricalcium)-437(phosphate)-429.1([)]TJ /F10 1 Tf 9.6734 0 TD (b)Tj /F4 1 Tf .5489 0 TD (-TCP]/bovine)Tj -18.4624 -1.1284 TD [(type)-473.8(I)-466(collagen)-470.1(matrix\))-471.8(increases)-469.5(vertebral)-474.9(bone)-468.3(mineral)]TJ 0 -1.1223 TD [(density)-338.6(in)-329.1(geriatric)-340.1(female)-338.1(baboons.)-333.8(Spine)-336.6(J)]TJ /F7 1 Tf 18.3526 0 TD (5,)Tj /F4 1 Tf 1.0857 0 TD [(13,)-335.8(2013.)]TJ -21.1887 -1.1284 TD [(29.)-500.5(Ho)16.4(pp)18(e)14(,)-170.8(A.)22.6(,)-170.8(G)0(u)22.5(l)0(d)18.6(a)0(l)20.5(,)-170.8(N.)22.6(S.)15.2(,)-164.7(a)0(n)19.9(d)-170.9(B)13.4(o)0(c)13.8(c)14(ac)15.7(ci)20.5(ni)18.6(,)-164.7(A)0(.)16.5(R)0(.)-157.5(A)-166.4(re)17.6(vi)18.6(ew)-158.5(of)-161.2(t)12.6(h)0(e)]TJ 1.7505 -1.1223 TD [(bi)18.6(ol)18.6(og)18(i)12.6(c)0(a)15.7(l)-255.7(re)17.6(sp)18.9(on)18(s)12.9(e)-260.5(to)-249.8(io)18.6(ni)18.6(c)-260.5(d)0(i)18.6(s)12.9(so)18.9(lu)18.6(ti)19.2(on)-250.4(pr)21.8(od)18(uc)19.9(ts)-248.9(fr)19.5(om)-249.9(bi)18.6(oa)19.9(c-)]TJ 0 -1.1284 TD [(ti)19.2(ve)-242.4(gl)18.6(as)20.8(se)14.7(s)-249.3(a)14(nd)-244.3(gl)18.6(a)14(s)0(s)13.7(-)0(c)23.7(e)0(r)17.6(a)0(m)20.3(i)0(c)14.4(s)12.9(.)-250.1(B)0(i)19.9(o)0(m)18.4(a)0(t)20.5(e)0(r)17.6(i)0(a)20.5(l)0(s)]TJ /F7 1 Tf 17.9744 0 TD [(11)18(,)]TJ /F4 1 Tf 1.4821 0 TD -.018 Tc [(3)-18(2)6.1(,)-268.1(2)-18(01)-18(1.)]TJ -21.207 -1.1223 TD 0 Tc [(30.)-500.5(Pa)17(yn)18(e)14(,)-335.5(K.)22.6(J.)19(,)-335.5(a)14(nd)-323.5(Ve)18.3(is)19.5(,)-335.5(A)0(.)-319(F)0(o)21.2(u)0(r)15.7(i)12.6(er)-324(t)12.6(r)0(a)17.6(n)0(s)18.9(f)0(o)15.7(r)0(m)-319.3(i)0(r)-325.3(s)12.9(pe)19.9(ct)14.4(ro)21.8(s)12.9(c)0(o)13.8(p)0(y)]TJ 1.7505 -1.1284 TD [(of)-222.2(co)19.9(l)12.6(l)0(a)14.4(g)0(e)19.9(n)-225.8(an)19.9(d)-231.9(g)0(e)19.9(l)0(a)20.5(t)0(i)19.2(n)-231.9(s)12.9(o)0(l)18.6(u)0(t)18.6(i)12.6(on)18(s:)-218.4(de)19.9(co)13.8(nv)24.1(ol)18.6(ut)18.6(i)12.6(o)0(n)-219.9(o)0(f)-222.2(t)12.6(he)-218(am)14.2(i)12.6(d)0(e)]TJ 0 -1.1223 TD [(I)-234.2(b)0(a)19.9(n)0(d)-226(f)0(o)21.8(r)-234.2(co)19.9(nf)21.8(or)15.7(m)12.5(a)0(t)20.5(i)0(o)18.6(n)0(a)19.9(l)-237.4(s)12.9(t)0(u)18.6(d)0(i)18.6(e)0(s)20.8(.)-231.8(Bi)19.9(op)18(ol)18.6(ym)18.4(er)17.6(s)]TJ /F7 1 Tf 18.8405 0 TD [(11)18(,)]TJ /F4 1 Tf 1.4638 0 TD [(27)24.1(,)-237.9(1)0(9)24.1(8)0(8)18(.)]TJ -22.0548 -1.1284 TD [(31.)-500.5(Barth,)-365.3(A.)-355.5(Infrared)-366.5(spectroscopy)-362(of)-362.5(proteins.)-359.5(Biochim)-362.2(Bio-)]TJ 1.7505 -1.1223 TD [(phys)-335.2(Acta)]TJ /F7 1 Tf 4.4524 0 TD (9,)Tj /F4 1 Tf 1.0796 0 TD [(1767,)-336.1(2007.)]TJ -7.2825 -1.1284 TD [(32.)-500.5(Autefage,)-639.3(H.,)-636.2(Briandme)]TJ 11.7349 .0427 TD ()Tj .3904 -.0427 TD [(sange,)-630.5(F.,)-637.5(Cazalbou,)-634.1(S.,)]TJ /F5 1 Tf 11.2043 0 TD [(et)-638.2(al.)]TJ /F4 1 Tf -21.5791 -1.1223 TD [(Adsorption)-723(and)-724.3(release)-726.2(of)-722.3(BMP-2)-723.6(on)-720(nanocrystalline)]TJ 0 -1.1284 TD [(apatite-coated)-295.7(and)-285.2(uncoated)-293.8(hydroxyapatite/beta-tricalcium)]TJ 0 -1.1223 TD [(phosphate)-459.6(porous)-453.7(ceramics.)-457.6(J)-456.7(Biomed)-454.1(Mater)-455.4(Res)-453.8(Part)-460.8(B)]TJ 0 -1.1284 TD [(Appl)-337(Biomater)]TJ /F7 1 Tf 6.4103 0 TD (2,)Tj /F4 1 Tf 1.0796 0 TD [(91,)-335.8(2009.)]TJ -9.2403 -1.1223 TD [(33.)-500.5(Shih,)-728.8(Y.R.,)-726.6(Hwang,)-734(Y.,)-727.7(Phadke,)-725.9(A.,)]TJ /F5 1 Tf 19.1028 0 TD [(et)-729.7(al.)]TJ /F4 1 Tf 3.2143 0 TD (Calcium)Tj -20.5666 -1.1284 TD [(phosphate-bearing)-424.5(matrices)-422.3(induce)-423.5(osteogenic)-420.7(differentia-)]TJ 0 -1.1223 TD [(tion)-408.1(of)-399.1(stem)-405.4(cells)-409.6(through)-399.2(adenosine)-408.9(signaling.)-405.5(Proc)-400.3(Natl)]TJ 0 -1.1284 TD [(Acad)-339.9(Sci)-330.2(U)-337.2(S)-332.4(A)]TJ /F7 1 Tf 7.0507 0 TD (3,)Tj /F4 1 Tf 1.0857 0 TD [(11,)-335.8(2014.)]TJ -9.8869 -1.1223 TD [(34.)-500.5(S)15.3(t)12.6(a)20.1(c)14(he)32.1(l)12.6(,)-219.6(I)15.8(.)0(,)-207.5(S)15.3(c)14(h)18.2(w)16.6(a)14(r)15.8(z)14(e)14(n)18.2(b)18.2(o)0(l)30.8(z)14(,)-219.6(U)0(.)28.7(,)-219.6(He)30.5(nl)30.8(e)14(,)-219.6(T)16.3(.)0(,)]TJ /F5 1 Tf 17.9805 0 TD [(e)14(t)-219.1(al)30.8(.)]TJ /F4 1 Tf 2.1591 0 TD [(C)19.5(r)15.8(os)31.1(s)12.9(-)15.8(L)16.3(i)18.7(nk)30.2(i)12.6(n)18.2(g)]TJ -18.3892 -1.1284 TD -.0168 Tc [(o)-16.8(f)-373.2(Type)-387.1(I)-385.2(C)-3.4(ol)-4.2(lagen)-382.9(w)-.2(i)-4.2(t)1.9(h)-389(M)2.1(i)-4.2(c)-2.8(robi)-4.2(al)-382.3(Trans)-3.9(g)1.4(l)-4.2(u)1.4(t)-4.2(a)3.3(m)-4.3(i)1.9(n)-16.8(a)15.3(s)-3.9(e)-2.8(:)-382.3(i)-4.2(den-)]TJ T* -.0143 Tc [(ti)4.4(cat)4.4(io)3.9(n)-392.6(o)-14.3(f)-382.9(C)5.2(ro)-14.3(s)16.8(s)-1.4(-Li)4.4(n)-14.3(k)15.9(i)-1.7(n)3.9(g)]TJ 10.9908 0 TD -.0169 Tc [(Si)-4.3(tes.)-401.2(Bi)-4.3(om)-4.4(acro)-16.9(m)13.7(o)1.3(l)-4.3(ecules)]TJ /F7 1 Tf 10.6554 0 TD -.0182 Tc (3,)Tj /F4 1 Tf 1.1101 0 TD 0 Tc [(11)30.2(,)]TJ -22.7562 -1.1223 TD [(20)30.2(1)18.2(0)0(.)]TJ -1.7505 -1.1284 TD [(35.)-500.5(Tenhuisen,)-608(K.S.,)-602.7(Martin,)-603.3(R.I.,)-605.3(Klimkiewicz,)-606.3(M.,)]TJ /F5 1 Tf 23.3662 0 TD [(et)-601.6(al.)]TJ /F4 1 Tf -21.6157 -1.1223 TD [(Formation)-442.2(and)-443.8(properties)-439.4(of)-441.7(a)-443.5(synthetic)-440.2(bone)-437.8(composite:)]TJ 0 -1.1284 TD [(hydroxyapatite-collagen.)-518.4(Journal)-506.2(of)-508.8(biomedical)-506(materials)]TJ 9.2951 0 0 9.2951 76.0252 191.6787 Tm (research)Tj /F7 1 Tf 3.6778 0 TD (7,)Tj /F4 1 Tf 1.0857 0 TD [(29,)-329.7(1995.)]TJ 20.4995 57.3938 TD [(36.)-500.5(He,)-420.9(C.,)-419.8(Xiao,)-426.7(G.,)-422.7(Jin,)-419.9(X.,)]TJ /F5 1 Tf 12.9121 0 TD [(et)-424.8(al.)]TJ /F4 1 Tf 2.6044 0 TD [(Electrodeposition)-424.2(on)-421.1(na-)]TJ -13.766 -1.1223 TD [(nobrous)-377.6(polymer)-372.5(scaffolds:)-383.6(rapid)-378.7(mineralization,)-377.8(tunable)]TJ 0 -1.1284 TD [(calcium)-249.9(phosphate)-258.3(composition)-252.8(and)-248.6(topography.)-251.5(Adv)-252.1(Funct)]TJ 0 -1.1223 TD (Mater)Tj /F7 1 Tf 2.7264 0 TD (20,)Tj /F4 1 Tf 1.5858 0 TD [(20,)-329.7(2010.)]TJ -6.0626 -1.1283 TD [(37.)-500.5(Specchia,)-404.6(N.,)-404.4(Pagnotta,)-407.8(A.,)-404.4(Cappella,)-413.9(M.,)]TJ /F5 1 Tf 19.4566 0 TD [(et)-406.5(al.)]TJ /F4 1 Tf 2.5678 0 TD [(Effect)-411.7(of)]TJ -20.2739 -1.1223 TD [(hydroxyapatite)-472.7(porosity)-465(on)-469.9(growth)-467.7(and)-468.2(differentiation)-476.3(of)]TJ 0 -1.1284 TD [(human)-333.9(osteoblast-like)-335.7(cells.)-336.5(J)-334.7(Mater)-333.4(Sci)]TJ /F7 1 Tf 16.7363 0 TD (3,)Tj /F4 1 Tf 1.0857 0 TD [(37,)-329.7(2002.)]TJ -19.5724 -1.1223 TD [(38.)-500.5(Chou,)-444.6(Y.F.,)-444.1(Huang,)-445.7(W.B.,)-448.8(Dunn,)-447.5(J.,)]TJ /F5 1 Tf 17.4926 0 TD [(et)-449.2(al.)]TJ /F4 1 Tf 2.6532 0 TD [(The)-445.7(effect)-450.6(of)]TJ -18.3953 -1.1284 TD [(biomimetic)-336.1(apatite)-341.3(structure)-341.7(on)-335.7(osteoblast)-335.7(viability,)-338.2(prolif-)]TJ 0 -1.1223 TD [(eration,)-333.9(and)-334(gene)-332.2(expression.)-339.3(Biomaterials)]TJ /F7 1 Tf 17.7183 0 TD (3,)Tj /F4 1 Tf 1.0796 0 TD [(26,)-335.8(2005.)]TJ -20.5483 -1.1284 TD [(39.)-500.5(Schwarz,)-343.8(M.L.R.,)-342.1(Kowarsch,)-343.6(M.,)-341.1(Rose,)-344.2(S.,)]TJ /F5 1 Tf 19.6456 0 TD [(et)-345.5(al.)]TJ /F4 1 Tf 2.4458 0 TD [(Effect)-344.6(of)]TJ -20.341 -1.1223 TD [(surface)-663.9(roughness,)-671(porosity,)-660.2(and)-669.5(a)-663.1(resorbable)-669.9(calcium)]TJ 0 -1.1284 TD [(phosphate)-569.4(coating)-563.3(on)-567.5(osseointegration)-574.3(of)-563.7(titanium)-569.9(in)-566.9(a)]TJ T* [(minipig)-334.3(model.)-333.4(J)-334.7(Biomed)-332.1(Mater)-339.5(Res)-331.8(Part)-338.8(A)]TJ /F7 1 Tf 18.3404 0 TD (3,)Tj /F4 1 Tf 1.0857 0 TD [(89,)-329.7(2009.)]TJ -21.1765 -1.1223 TD [(40.)-500.5(Koutsopoulos,)-579.9(S.)-582.5(Synthesis)-584.9(and)-584.1(characterization)-595.2(of)-582(hy-)]TJ 1.7505 -1.1284 TD [(droxyapatite)-588.3(crystals:)-594.1(a)-589.9(review)-592(study)-590.9(on)-585.8(the)-589.6(analytical)]TJ 0 -1.1223 TD [(methods.)-332.8(J)-334.7(Biomed)-332.1(Mater)-339.5(Res)-331.8(Part)-338.8(A)]TJ /F7 1 Tf 15.7665 0 TD (4,)Tj /F4 1 Tf 1.0857 0 TD [(62,)-329.7(2002.)]TJ -18.6027 -1.1284 TD [(41.)-500.5(Z)16.3(h)0(u)30.2(,)-250.1(Y.)28.7(,)-256.2(Z)16.3(h)18.2(u)0(,)-238.1(R)13.4(.,)-238(M)12.8(a)14(,)-250.1(J)12.9(.)18.2(,)]TJ /F5 1 Tf 11.6373 0 TD [(e)14(t)-249.6(al)30.8(.)]TJ /F4 1 Tf 2.2201 0 TD -.0139 Tc [(In)-264.1(v)-13.9(i)16.9(tro)-264.1(c)0(ell)-263.5(p)-13.9(r)14(o)-13.9(l)16.9(i)-1.3(feratio)4.3(n)-264.1(e).1(v)-13.9(a)12.1(l-)]TJ -12.107 -1.1223 TD 0 Tc [(u)18.2(a)14(t)12.6(i)18.7(on)-396.7(of)-393(po)30.2(r)15.8(o)0(u)24.1(s)-407.9(n)18.2(a)14(no)30.2(-)15.8(z)14(i)12.6(r)15.8(c)14(o)0(n)30.2(i)12.6(a)-406.9(s)12.9(c)14(a)14(f)15.8(fo)27.9(l)12.6(d)18.2(s)-407.9(w)0(i)23.1(t)18.7(h)-408.8(d)0(i)30.8(f)0(f)25.6(e)14(r)15.8(e)14(n)18.2(t)-414.3(p)18.2(o)0(-)]TJ 0 -1.1284 TD -.0152 Tc [(rosity)-253.2(fo)-15.2(r)-237.4(b)3(o)-15.2(n)15(e)-251.3(tissu)-15.2(e)-239.3(e)-1.2(ng)-15.2(i)15.6(n)-15.2(e)10.8(e)-1.2(ring)-15.2(.)-235(B)-1.8(iomed)-247.1(M)-2.4(ater)]TJ /F7 1 Tf 19.359 0 TD -.0182 Tc (5,)Tj /F4 1 Tf .9698 0 TD -.0302 Tc [(1)-12(0)-30.2(,)-250(2)-30.2(01)-30.2(5.)]TJ -22.0792 -1.1223 TD 0 Tc [(42.)-500.5(Ozeki,)-431.1(M.,)-432.6(Kuroda,)-436(S.,)-430.1(Kon,)-435.1(K.,)]TJ /F5 1 Tf 15.8641 0 TD [(et)-430.9(al.)]TJ /F4 1 Tf 2.6227 0 TD [(Differentiation)-441.3(of)]TJ -16.7363 -1.1284 TD [(bone)-633(marrow)-639.1(stromal)-639.4(cells)-641.4(into)-633.8(osteoblasts)-639.9(in)-640.1(a)-632.6(self-)]TJ 0 -1.1223 TD [(assembling)-287.1(peptide)-282.7(hydrogel:)-287.3(in)-286.4(vitro)-282.5(and)-285.2(in)-286.4(vivo)-286.7(studies.)-282.9(J)]TJ 0 -1.1284 TD [(Biomater)-332.3(Appl)]TJ /F7 1 Tf 6.4042 0 TD (7,)Tj /F4 1 Tf 1.0857 0 TD [(25,)-329.7(2011.)]TJ -9.2403 -1.1223 TD .0106 Tc [(43.)-501.8(L)2.5(egeros)10.6(,)-312(R)10.6(.)-6.6(Z)-3.6(.)-300.5(P)1.5(ropert)10.6(i)-6.8(e).2(s)-299.7(o)-1.7(f)-302.9(o)4.4(steoconductive)-304.8(b)-1.7(i)10.6(o)-7.4(mat)10.6(e)-11.6(rial)10.6(s)-6.5(:)]TJ 1.781 -1.1284 TD .011 Tc [(cal)11(c)-11.2(i)11(u)-7(m)-562(phosphates.)-562.4(C)11(l)-5.7(i)-.8(n)-562.5(O)3.2(rt)11(h)-7(o)-1.3(p)-562.5(R)11(e)-10.5(l)11(a)-5.1(t)-561.9(Res)]TJ /F7 1 Tf 19.8103 0 TD .0062 Tc [(39)-6.1(5,)]TJ /F4 1 Tf 2.3482 0 TD .0123 Tc [(395)6.1(,)]TJ -22.1585 -1.1223 TD .0062 Tc [(20)-6.1(02)-6.1(.)]TJ -1.781 -1.1284 TD 0 Tc [(44.)-500.5(Ramirezrodr)]TJ 6.8006 .0427 TD ()Tj .2989 -.0427 TD [(guez,)-490.9(G.B.,)-488.7(Montesi,)-490.5(M.,)-487.5(Panseri,)-489(S.,)]TJ /F5 1 Tf 16.3764 0 TD [(et)-485.8(al.)]TJ /F4 1 Tf -21.7255 -1.1223 TD [(Biomineralized)-334.3(recombinant)-338.2(collagen-based)-334.4(scaffold)-336(mim-)]TJ 0 -1.1284 TD [(icking)-333.3(native)-331.4(bone)-334.1(enhances)-340.4(mesenchymal)-333.3(stem)-338.3(cell)-331.1(inter-)]TJ 0 -1.1223 TD [(action)-258.2(and)-260.8(differentiation.)-269(Tissue)-260.8(Eng)-258.5(Part)-265.6(A)]TJ /F7 1 Tf 18.2855 0 TD (23,)Tj /F4 1 Tf 1.5126 0 TD [(23,)-256.5(2017.)]TJ -21.5486 -1.1284 TD [(45.)-500.5(Zhang,)-537.5(W.,)-535.3(Liao,)-536.8(S.S.,)-536.9(and)-535.3(Cui,)-535.3(FZ.)-535.8(Hierarchical)-545.7(Self-)]TJ 1.7505 -1.1223 TD [(Assembly)-351.8(of)-356.4(Nano-Fibrils)-354(in)-353.5(Mineralized)-359.8(Collagen.)-354.8(Chem)]TJ 0 -1.1284 TD (Mater)Tj /F7 1 Tf 2.7264 0 TD (16,)Tj /F4 1 Tf 1.5858 0 TD [(15,)-329.7(2003.)]TJ -6.0626 -1.1223 TD [(46.)-500.5(Poinern,)-461.3(G.E.,)-461.4(Brundavanam,)-466.2(R.K.,)-464.3(Mondinos,)-462.6(N.,)]TJ /F5 1 Tf 23.5004 0 TD [(et)-461.4(al.)]TJ /F4 1 Tf -21.7499 -1.1284 TD [(Synthesis)-273.9(and)-273(characterisation)-279.1(of)-271(nanohydroxyapatite)-276.2(using)]TJ 0 -1.1223 TD [(an)-388.7(ultrasound)-396.3(assisted)-396.1(method.)-394.5(Ultrason)-391.5(Sonochem)]TJ /F7 1 Tf 21.5791 0 TD (4,)Tj /F4 1 Tf 1.1406 0 TD (16,)Tj -22.7197 -1.1284 TD 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