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[(strated)-315.3(multilineage)-312.3(differentiation)-320.1(potential)-312.3([11,12].)-312.9(In)-307.4(this)]TJ
0 -1.1174 TD
[(study,)-394.8(we)-390.1(studied)-394.8(the)-396.3(characteristics)-397.9(of)-393.8(stem)-392.6(cells)-393.8(isolated)]TJ
0 -1.1116 TD
[(from)-339.6(the)-338.7(salivary)-345.2(gland,)-338.5(such)-339.4(as)-343(morphology,)-338.3(proliferation,)]TJ
T*
[(surface)-385.3(markers,)-388.8(and)-384.6(multilineage)-387.2(differentiation)-389.2(potential,)]TJ
T*
[(of)-301.7(healthy)-298.3(individuals)-301.6(and)-298.2(patients)-305.1(with)-299.3(SS,)-299.1(to)-296.1(elucidate)-302.9(the)]TJ
0 -1.1174 TD
[(pathogenesis)-471.6(of)-462.9(SS,)-460.3(and)-465.2(to)-468.9(uncover)-465(new)-466.1(avenues)-466(for)-464(the)]TJ
0 -1.1116 TD
[(treatment)-337.5(of)-336.2(SS)-330.2(by)-336.3(using)-333.7(labial)-331.9(gland)-335.1(stem)-335(cells.)]TJ
/F1 1 Tf
9.9626 0 0 9.9626 62.022 271.8424 Tm
[(Mater)-8.1(ials)-337.1(and)-336.9(Methods)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 253.9842 Tm
[(This)-253.2(study)-247.3(was)-252.8(approved)-258.8(by)-249.9(the)-252.3(institutional)-252.3(review)-251.8(board)]TJ
-1.0137 -1.1116 TD
[(of)-382.3(Peking)-382.1(University)-381.8(School)-382.1(of)-376.5(Stomatology)-383.4(\(PKUSSIRB-)]TJ
T*
[(201520016\).)-555.8(All)-551.6(patients)-558.5(gave)-553.9(written)-557.3(informed)-558.4(consent)]TJ
T*
[(before)-337.2(their)-335.2(participation)-335.2(in)-336.4(this)-332.8(study.)]TJ
/F9 1 Tf
9.9626 0 0 9.9626 62.022 196.044 Tm
[(Prima)-9.9(ry)-333.6(culture)-340.9(of)-333.6(labi)-7.8(al)-334(gland)-339.6(stem)-337.8(cells)]TJ
0 -1.2007 TD
[(from)-339.8(patien)-7.4(ts)-334.3(with)-336.5(SS)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 72 166.2236 Tm
[(SS)-340.5(groups.)]TJ
/F4 1 Tf
9.843 0 0 9.843 120.0189 166.2236 Tm
[(Five)-339.6(female)-270.6(patients)-270.5(with)-264.7(SS)-266.8(\(mean)-268.2(age:)-266.1(47.5)]TJ
-5.8922 -1.1116 TD
[(years\),)-311.6(who)-303.7(visited)-308.6(the)-309.9(Department)-310.6(of)-307.4(Oral)-311.8(Medicine)-307.2(or)-307.4(the)]TJ
T*
[(Department)-402.7(of)-399.6(Maxillofacial)-406.4(Surgery)-400.3(at)-400.9(Peking)-399.4(University)]TJ
0 -1.1174 TD
[(School)-174.8(of)-169.2(Stomatology)-170.3(from)-172.6(March)-170(2015)-171.4(to)-169.4(October)-172.3(2015)-171.4(and)]TJ
0 -1.1116 TD
[(were)-508.7(clinically)-516.2(and)-505.6(pathologically)-514.9(conrmed)-507.5(to)-509.2(have)-507.8(SS)]TJ
T*
[(\(according)-423.5(to)-417(2002)-419.1(AmericanEuropean)-425.3(Consensus)-420.5(Group)]TJ
0 -1.1174 TD
[(classication)-191.7(criteria)-196.9([13]\),)-187.4(were)-186.1(recruited)-192.1(in)-186.6(this)-188.8(group.)-187.5(After)]TJ
0 -1.1116 TD
[(obtaining)-178.5(informed)-178.3(consent,)-180.4(the)-171.6(labial)-182.1(glands)-174.7(of)-175(these)-180.6(patients)]TJ
T*
[(were)-261(resected)-266.8(under)-260.1(sterile)-269.4(conditions.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 72 67.6346 Tm
[(Healthy)-265.7(control)-267.5(group.)]TJ
/F4 1 Tf
9.843 0 0 9.843 163.7291 67.6346 Tm
[(Five)-276.2(female)-201.5(non-SS)-196.3(patients)-201.4(\(mean)]TJ
-10.3329 -1.1174 TD
[(age:)-415.8(45.6)-415.7(years\),)-421(who)-413.1(visited)-418(the)-419.3(Department)-420(of)-416.9(Oral)-415.5(and)]TJ
25.504 67.9184 TD
[(Maxil)-7.8(lofacial)-387(Surgery)-383(at)-383.6(Peking)-387.9(University)-387.6(School)-382.1(of)-388.1(Sto-)]TJ
0 -1.1174 TD
[(matology)-460.7(from)-454.8(March)-458(2015)-453.7(to)-451.6(October)-460.3(2015)-453.7(and)-453.7(needed)]TJ
0 -1.1116 TD
[(surgery)-273.6(to)-261.5(remove)-269.3(lip)-268.5(mucocele,)-271.7(agreed)-269.3(to)-267.3(participate)-270.7(in)-267.3(this)]TJ
T*
[(study.)-440.9(After)-439.6(obtaining)-437.7(their)-433.1(informed)-437.5(consent,)-439.6(their)-438.9(labial)]TJ
T*
[(glands)-266.9(were)-261(resected)-266.8(under)-260.1(sterile)-263.6(conditions.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 323.0361 670.3369 Tm
[(Isolation)-422.4(and)-422.2(culture)-428(of)-420.4(labial)-426.4(gland)-418.9(stem)-426.3(cells)-422.6(by)-425.1(using)-426.4(the)]TJ
-1.1128 -1.2203 TD
[(monolayer)-312.5(method.)]TJ
/F4 1 Tf
9.843 0 0 9.843 391.8046 659.3951 Tm
-.0181 Tc
[(Af)3.9(ter)-307.2(t)3.7(h)3.8(e)-308.3(r)3.9(e)-3.1(s)4(e)2.7(ct)3.7(i)3.7(o)-1.9(n)-301.4(o)-1.9(f)-301.4(l)-2(ab)3.8(ial)-307.3(g)3.8(lan)3.8(d)-307.2(t)3.7(i)-2.1(s)4(s)4(u)-1.9(es,)]TJ
-8.0002 -1.1116 TD
-.021 Tc
[(the)-6(y)-575(we)-6(re)-581.9(imme)-6(diate)-6(l).8(y)-575(s)-4.6(oa)-6(ked)-580.8(i).8(n)-580.8(m)-.3(esenc)-6(hym)-6(al)-580.9(ste)-6(m)-576.3(ce)-6(ll)]TJ
0 -1.1174 TD
-.0156 Tc
[(me)5.2(d)6.3(i).4(u)6.3(m)-398.1(\()-74.3(M)-.3(S)6.5(C)5.3(M\))-391(an)6.3(d)-396.8(t)6.2(ra)5.2(n)6.3(s).8(f)6.4(e)5.2(rr)6.4(ed)-391.1(to)-391.1(a)-398(b)6.3(io)6.3(sa)5.2(f)6.4(e)-.6(t)6.2(y)-396.8(c)5.2(a)5.2(b).6(i)6.2(n)6.3(et)6.2(.)-393.4(F).7(i)6.2(-)]TJ
0 -1.1116 TD
-.0173 Tc
[(br)4.7(ou)4.6(s)-461.7(t)4.5(is)4.8(su)4.6(es,)-458.5(c)-2.3(ap)4.6(su)4.6(l)4.5(e)-2.3(s)4.8(,)-464.2(an)4.6(d)-461.9(b)-1.1(l)4.5(o)4.6(od)-461.9(v)4.6(e)3.5(ss)4.8(el)4.5(s)-461.7(a)3.5(ro)4.6(un)4.6(d)-461.9(t)4.5(he)-457.3(lab)4.6(i)-1.3(al)]TJ
T*
-.0179 Tc
[(gl)3.9(an)4(d)4(s)-249.2(were)-244.8(remo)4(ved)-243.6(b)-1.7(y)-243.6(u)-1.7(s)4.2(i)3.9(ng)-243.6(op)4(h)4(t)-1.8(h)4(a)2.9(lmi)3.9(c)-250.5(s)4.2(c)2.9(is)4.2(so)4(r)4.1(s)-1.5(.)-246(T)2.9(h)4(e)-250.6(t)3.9(i)-1.8(s)4.2(s)4.2(u)-1.7(es)]TJ
0 -1.1174 TD
-.0215 Tc
[(w)-6.2(e)-.7(r)-5.3(e)-311.7(mi)-5.4(nce)-6.5(d)-310.6(in)-5.3(to)-316.3(1-m)-6.5(m)]TJ
7.3823 0 0 6.5614 406.0345 608.9951 Tm
0 Tc
(3)Tj
9.843 0 0 9.843 412.6109 604.5731 Tm
-.0201 Tc
[(pie)-5.1(ces)-314.7(by)-314.9(using)-314.9(o)1.8(phthalm)-5.1(i)1.7(c)-310.3(s)-3.7(cissors,)]TJ
-10.114 -1.1116 TD
-.0198 Tc
[(pla)-4.8(ced)-406.8(in)-406.8(10-cm)-408(cell)-406.9(c)1(ulture)-408(di)]TJ
12.5216 0 TD
-.0194 Tc
[(shes,)-408.7(a)1.4(nd)-406.4(washed)-406.4(three)-407.6(t)2.4(imes)]TJ
-12.5216 -1.1116 TD
-.0214 Tc
[(w)-6.1(i).4(t)-5.3(h)-327.8(P)-5.1(B)-.5(S)-333.4(a)-.6(nd)-333.5(on)-5.2(ce)-334.7(wit)-5.4(h)-327.8(M)-6.1(S).7(CM.)-335.9(T)-.7(he)-334.7(med)-5.2(i).4(um)-334.7(wa)-6.4(s)-327.6(a)-6.4(spi)-5.4(r).6(a)-6.4(t).4(ed)-5.2(,)]TJ
T*
[(a)-6.5(n).4(d)-333.6(t)-5.5(he)-334.8(d)-5.3(i).3(sh)-5.3(es)-333.4(w)-6.2(e)-.7(re)-334.8(p)-5.3(l).3(ac)-6.5(ed)-333.6(i)-5.4(n)-333.6(a)-334.8(c)-.7(e)-6.5(ll-)-5.3(hat)-5.4(c)-.7(hi)-5.4(ng)-333.6(b)-5.3(o).4(x)-333.6(a)-6.5(t)-333.8(3).4(7)]TJ
/F8 1 Tf
21.8926 0 TD
0 Tc
()Tj
/F4 1 Tf
.3801 0 TD
-.0209 Tc
(C.)Tj
-21.2591 -1.1174 TD
.3578 Tc
[(A)373.1(f)379.8(t)379.6(e)372.8(rt)379.6(h)379.7(e)-1.6(t)373.8(i)379.6(s)374.2(s)379.9(u)379.7(e)-1.6(b)374(l)379.6(o)374(c)378.6(ka)378.6(t)373.9(t)379.6(a)378.6(c)372.8(h)379.7(e)378.6(dt)373.9(ot)379.6(h)379.7(e)-1.6(w)373.1(a)378.6(l)379.6(l)373.9(,1)374(0)212.7(m)378.5(LM)373.1(S)379.9(C)378.7(M)]TJ
-1.0137 -1.1116 TD
-.0215 Tc
[(w)-6.2(a)-.7(s)-442.9(s)-5.1(low)-6.2(l).3(y)-443.1(a)-.7(dd)-5.3(ed)-443(fo)-5.3(r)-443(p).4(ri)-5.4(mar)-5.3(y)-443.1(cel)-5.4(l)-437.4(c)-6.5(ult)-5.4(u).4(r)-5.3(e)-438.5(a)-6.5(t)-443.2(37)]TJ
/F8 1 Tf
19.6867 0 TD
0 Tc
()Tj
/F4 1 Tf
.3801 0 TD
-.0219 Tc
[(C)-438.7(a)-6.9(nd)-443.4(5%)]TJ
-20.0668 -1.1116 TD
-.0152 Tc
(CO)Tj
7.3823 0 0 6.5614 326.3243 537.2219 Tm
0 Tc
(2)Tj
9.843 0 0 9.843 329.8393 538.866 Tm
-.0217 Tc
[(.)-376.5(A)-.6(f)-5.5(t).1(er)-379.9(the)-381.1(cel)-5.6(ls)-379.7(gre)-6.7(w)-375(ou)-5.5(t)-374.3(o)-5.5(f)-374.1(t).1(h)-5.5(e)-375.3(t)-5.7(i).1(ss)-5.3(ue)-381.1(blo)-5.5(c)-.9(k,)-376.5(w)-6.4(e)-375.3(st)-5.6(art)-5.6(e)-.9(d)]TJ
-1.7049 -1.1174 TD
-.0215 Tc
[(o)-5.3(b).4(s)-5.1(e)-.7(rv)-5.3(ing)-281.8(cel)-5.4(lul)-5.4(a)-.7(r)-276(m)-6.5(orp)-5.3(hol)-5.4(ogy)-5.3(;)-276.2(w)-.4(e)-277.2(b)-5.3(eg)-5.3(an)-276(o)-34.1(b)-39.9(s)-33.9(e)-41(r)-34.1(v)-39.9(a)-35.3(t)-40(i)-34.2(o)-34.1(n)-316.3(o)-34.1(n)-310.6(d)-39.9(a)-35.3(y)]TJ
0 -1.1116 TD
.0146 Tc
[(5.)-276.8(When)-280.2(80%)-275.5(c)-4.9(ell)-274.6(c)-4.9(onue)-4.9(nc)-4.9(e)-275.6(w)-4.6(as)-280(a)6.6(c).8(h)7.7(i)1.9(e)6.6(v)2(ed)7.7(,)-271(w)1.1(e)-275.6(p)7.7(erf)7.8(o)2(r)7.8(m).8(e)6.6(d)]TJ
T*
.0119 Tc
[(pas)5.2(s)-.5(age)-537.5(c)-1.9(u)5(l)-.8(t)4.9(i)-.8(v)5(a)-1.9(ti)4.9(on)5(.)-538.7(T)3.9(he)-537.5(cu)5(lt)4.9(ure)-537.5(med)5(i)-.8(u)5(m)-537.6(was)-541.9(r)5.1(ef)5.1(reshed)]TJ
0 -1.1174 TD
0 Tc
[(e)-13.8(v)-12.6(e)-8(r)-12.6(y)-577.1(3)-577.1(d)-12.6(a)-8(y)-12.6(s)-6.7(,)-579.4(a)-13.8(n)-6.9(d)-577.1(t)-12.7(h)-6.9(i)-12.7(r)-12.6(d)-6.9(-)-12.6(g)-6.9(e)-13.8(n)-6.9(e)-13.8(r)-6.8(a)-13.8(t)-12.7(i)-7(o)-12.6(n)-577.1(cel)11.5(ls)-555.1(wer)12(e)-566.7(use)13(d)-559.8(for)]TJ
0 -1.1116 TD
[(sub)14.2(seq)13(uen)12.8(t)-295(exp)12.8(eri)12.7(me)12.7(nts)-285.5(aft)12.7(er)-285.6(80%)-286.6(co)13.9(nu)14.1(enc)11.7(e)-296(w)0(a)13(s)-294.7(ach)11.7(iev)12.7(ed.)]TJ
/F9 1 Tf
9.9626 0 0 9.9626 313.0582 462.7841 Tm
[(Stu)-9(dy)-330.8(of)-339.2(prolife)-8.4(ration)-334.5(ch)-6.4(aracte)-7(ristics)]TJ
0 -1.2007 TD
[(of)-339.2(labial)-341.8(gland)-333.9(stem)-337.8(c)-6.5(ells)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 433.0204 Tm
[(Samples)-350.9(of)-347.8(third-generation)-349.8(labial)-349.1(gland)-346.6(stem)-352.3(cells)-347.7(from)]TJ
-1.0137 -1.1174 TD
[(SS)-393.5(and)-384.6(control)-389.2(groups)-392.3(were)-387.7(subjected)-393.6(to)-388.2(digestion,)-389.1(centri-)]TJ
0 -1.1116 TD
[(fugation,)-238.3(and)-234.9(counting)-234.9(after)-238.3(re-suspension.)-238.4(A)-232.4(100-)]TJ
/F10 1 Tf
20.1014 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(L)-232.7(aliquot)]TJ
-20.6543 -1.1116 TD
[(of)-405.3(the)-402(cell)-404.4(suspension)-403.4(solution)-406.4(was)-402.6(added)-405.3(to)-399.7(each)-405.3(well)-404.1(of)]TJ
0 -1.1174 TD
[(a)-520.6(96-well)-516.8(plate,)-518.4(with)-518.1(ve)-516.9(replicates)-522.7(for)-515.9(each)-520.5(group)-518.1(and)]TJ
/F10 1 Tf
0 -1.1116 TD
(*)Tj
/F4 1 Tf
1.0022 0 TD
[(3,000)-341.9(cells)-347.7(per)-344.3(well.)-344.2(From)-345.3(day)-338.5(1)-346.7(t)0(o)-342.2(1)0(0)-342(after)-347.7(inoculation,)]TJ
-1.0022 -1.1116 TD
[(a)-267.2(CCK-8)-267.6(test)-270.6(was)-264.3(conducted)-267(each)-267.1(day.)-267.1(Optical)-270.5(density)-268.1(\(OD\))]TJ
T*
[(at)-280(wavelength)-274.8(450)-170.3(nm)-274.1(was)-275.9(recorded)-277.2(for)-273.9(each)-278.6(well.)-275.1(With)-277.6(cell)]TJ
0 -1.1174 TD
[(cultivation)-343.4(time)-341.1(as)-337.2(abscissas)-346.8(and)-338.5(OD)-338(values)-337.2(as)-343(ordinates,)-338.3(a)]TJ
0 -1.1116 TD
[(cell)-341.1(proliferation)-333.9(curve)-336.1(was)-333.5(plotted.)]TJ
/F9 1 Tf
9.9626 0 0 9.9626 313.0582 312.4346 Tm
[(Flow)-344.9(cytometric)-343.8(detection)-341.7(of)-333.6(the)-339.2(surface)-337.8(mark)-7(ers)]TJ
0 -1.2007 TD
[(of)-339.2(labial)-341.8(gland)-333.9(stem)-337.8(c)-6.5(ells)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 282.6708 Tm
[(Third-generation)-287.6(labial)-285.8(gland)-283.3(stem)-277.4(cells)-284.4(from)-282(the)-281.1(test)-287.9(and)]TJ
-1.0137 -1.1116 TD
[(control)-406.5(groups)-398(were)-399.2(subjected)-405.1(to)-399.7(digestion,)-400.6(centrifugation,)]TJ
0 -1.1174 TD
[(and)-465.2(counting)-465.3(after)-468.7(re-suspension.)-468.8(Cells)-462.8(were)-468.3(resuspended)]TJ
0 -1.1116 TD
[(to)-468.9(a)-463(concentration)-468.6(of)-468.7(1)]TJ
/F8 1 Tf
10.0622 0 TD
()Tj
/F4 1 Tf
.7142 0 TD
(10)Tj
7.3823 0 0 6.5614 428.9952 254.1543 Tm
(7)Tj
9.843 0 0 9.843 434.3243 249.7889 Tm
[(cells/mL,)-472.2(and)-465.2(100)]TJ
/F10 1 Tf
7.8159 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(L)-463.1(o)0(f)-468.7(the)]TJ
-20.6889 -1.1116 TD
[(cell)-369.9(suspension)-368.8(was)-362.2(added)-370.7(to)-365.2(round-bottomed)-363.6(wells)-370.5(of)-365(mi-)]TJ
0 -1.1174 TD
[(crowell)-955.7(plates.)-957(Fluorescent-label-conjugated)-962(antibodies)]TJ
0 -1.1116 TD
[(again)-8(st)-307.4(CD29,)-315(CD34,)-315(CD44,)-315(CD)-315.1(45,)-310.9(CD73,)-315(CD90,)-315(CD105,)]TJ
T*
[(CD49f,)-287.3(CD106,)-281.6(and)-286.7(CD117)-283.9(were)-284(added)-284.3(and)-280.9(the)-286.8(plates)-285.5(were)]TJ
0 -1.1174 TD
[(incubated)-388(for)-308.5(2045)-166.8(min)-309.9(on)-313.2(ice,)-308.7(in)-307.6(the)-315.6(dark.)-308.5(The)-310.9(cells)-313.2(were)]TJ
0 -1.1116 TD
[(washed)-602.9(twice)-596.4(with)-598.8(100200)]TJ
/F10 1 Tf
12.4122 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(L)-595.5(o)0(f)-595.4(stain)-594.2(buffer)-600.9(and)-592(re-)]TJ
-12.9651 -1.1116 TD
[(suspended)-219.2(in)-215.4(200)]TJ
/F10 1 Tf
7.0441 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(L)-215.4(o)0(f)-215.3(stain)-214.1(buffer.)-218.5(The)-213(labeled)-218.8(cell)-214.3(samples)]TJ
-7.5971 -1.1174 TD
[(were)-266.8(analyzed)-261.3(by)-261.4(ow)-263.2(cytometry.)]TJ
/F9 1 Tf
9.9626 0 0 9.9626 313.0582 140.2016 Tm
[(Col)-8.6(ony-fo)-7(rming)-340.2(efciency)-340.5(assay)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 122.3433 Tm
[(Third-generation)-350.9(cells)-342(were)-341.6(sampl)-7.8(ed)-337.4(during)-346.5(an)-337.4(exponen-)]TJ
-1.0137 -1.1116 TD
[(tial)-391.9(growth)-393.5(period,)-391.4(and)-390.4(they)-391.6(were)-393.5(suspended)-392(in)-394(MSCM)-387.2(to)]TJ
T*
[(obtain)-290.3(a)-284.5(single-cell)-286.6(suspension)-288.2(with)-282(a)-284.5(single)-7.8(-cell)-284.5(percentage)]TJ
0 -1.1174 TD
[(above)-457.1(95%;)-454.9(the)-453.8(cell)-456.2(concentration)-457.1(was)-454.4(adjusted)-454.7(to)-451.6(l)]TJ
/F8 1 Tf
22.4168 0 TD
()Tj
/F4 1 Tf
.72 0 TD
(l0)Tj
7.3823 0 0 6.5614 548.5038 93.8834 Tm
(4)Tj
9.843 0 0 9.843 313.0582 78.5197 Tm
[(cells/mL.)-512.5(Cells)-428.2(were)-433.8(inoculated)-435.3(into)-430.9(a)-434.2(six-well)-431.5(plate)-434.3(at)-429.7(a)]TJ
0 -1.1116 TD
[(densit)-7.8(y)-179.6(o)0(f)-180.7(100)-181.9(cells/well,)-186.3(and)-183(six)-181.8(replicate)-182(wells)-186.2(were)-186.1(used)-178.2(for)]TJ
T*
[(each)-226.8(group)-224.3(of)-226.8(cells.)-224.4(The)-224.5(plates)-227.9(were)-226.4(placed)-223.3(in)-227(an)-222.2(incubator)-225.6(at)]TJ
/F1 1 Tf
8.9663 0 0 8.9663 62.022 749.6502 Tm
(1172)Tj
42.4584 0 TD
[(WANG,)-336.1(WANG,)-336.1(AND)-337.2(HUA)]TJ
ET
endstream
endobj
107 0 obj
<>stream
/GS1 gs
BT
/F4 1 Tf
9.843 0 0 9.843 59.7543 725.1588 Tm
0 0 0 rg
0 Tc
0 Tw
(37)Tj
/F8 1 Tf
1.0022 0 TD
()Tj
/F4 1 Tf
.3974 0 TD
[(C)-434.1(under)-438.6(5%)-441(CO)]TJ
7.3823 0 0 6.5614 142.1858 723.5148 Tm
(2)Tj
9.843 0 0 9.843 145.9275 725.1588 Tm
[(.)-435.4(When)-439.8(visible)-441(clones)-435.1(appeared)-445.4(after)]TJ
-8.7548 -1.1174 TD
[(7)-266(days)-264.6(of)-267.1(cultivation,)-266.2(the)-269.5(culture)-263.7(was)-270.1(stopped)-262.2(and)-269.4(the)-263.8(clones)]TJ
0 -1.1116 TD
[(were)-410.7(subjected)-416.6(to)-405.5(GIEMSA)-407.7(staini)-8(ng)-405.4(\(Sigma-Aldrich\),)-410.4(fol-)]TJ
T*
[(lowed)-266.9(by)-255.6(air)-263.7(drying)-260.1(and)-263.6(counting)-257.9(of)-261.4(clones.)]TJ
/F9 1 Tf
9.9626 0 0 9.9626 59.7543 666.255 Tm
[(Diffe)-10(rentiat)-8(ion)-333.9(of)-333.6(labi)-7.8(al)-334(gland)-339.6(stem)-337.8(cells)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 69.7323 648.4534 Tm
[(Osteogenic)-552.4(differentiation.)]TJ
/F4 1 Tf
9.843 0 0 9.843 177.8456 648.4534 Tm
[(Third-generation)-541(labial)-533.5(gl)20.7(an)19.7(d)]TJ
-11.9975 -1.1174 TD
[(st)15.1(em)-338.6(ce)18.5(ll)20.6(s)-352.2(f)0(r)20.9(o)0(m)-337.5(t)0(h)20.7(e)-347.8(SS)-341.7(an)19.7(d)-346.7(c)0(o)19.7(n)0(t)14.9(r)0(o)20.9(l)-346.8(gr)20.9(ou)15.1(ps)-336.1(an)19.7(d)-346.7(h)0(u)15.1(m)0(a)18.4(n)-346.7(bo)20.8(ne)]TJ
0 -1.1116 TD
[(ma)18.4(rr)15.1(ow)-250.7(MS)20.1(Cs)-250.7(\(h)20.9(BM)18.9(SC)20(s,)-252(No)14.2(.)-256.9(1)0(2)20.8(9)0(7)15.1(6)0(;)-250(S)0(c)19.8(i)0(e)19.6(n)0(c)19.7(e)0(l)19.6(l)-260.4(Re)18.6(se)14.1(ar)19.7(ch)-251(La)18.5(-)]TJ
T*
[(bo)15.1(ra)19.7(to)20.7(ri)20.7(es)19.9(\))-150.8(w)0(e)18.8(r)0(e)-141.6(i)0(n)20.7(o)0(c)13.9(u)0(l)20.7(a)0(t)19.5(e)0(d)-141.6(a)0(t)-141.7(a)-152(de)19.7(ns)21(it)20.6(y)-150.8(o)0(f)-140.4(5)]TJ
/F8 1 Tf
16.7953 0 TD
()Tj
/F4 1 Tf
.7084 0 TD
(10)Tj
7.3823 0 0 6.5614 241.6818 619.9368 Tm
(4)Tj
9.843 0 0 9.843 246.8976 615.5715 Tm
[(ce)18.5(ll)20.6(s/)20.9(we)18.8(ll)-140.7(in)-140.6(a)]TJ
-19.0128 -1.1174 TD
[(si)15.1(x-)20.9(we)18.8(ll)-359.6(pl)20.7(at)19.5(e)-370.9(a)0(n)19.7(d)-369.7(ce)18.5(ll)-359.6(cl)13.8(im)19.5(bi)20.7(ng)-359.3(sl)20.9(id)20.7(es)-360.3(in)-359.4(th)15(e)-370.9(s)0(i)20.9(x)0(-)20.9(w)0(e)18.8(l)0(l)-359.6(p)0(l)20.7(a)0(t)19.5(e)0(.)]TJ
0 -1.1116 TD
[(Af)14.2(te)19.6(r)-179.6(2)0(4)20.8(-)0(h)-169.2(c)0(u)19.7(l)0(t)20.6(i)0(v)20.7(a)0(t)19.5(i)0(o)20.7(n)-179.6(in)-169.4(MS)20.1(CM)18.9(,)-182(t)0(h)20.7(e)-180.8(me)18.4(di)20.7(um)-170.5(in)-169.4(th)20.7(e)-175(o)0(s)15.3(t)0(e)19.6(o)0(g)20.8(e)0(n)19.7(i)0(c)]TJ
T*
[(in)15(du)20.8(ct)19.5(io)20.7(n)-317.9(g)0(r)20.9(o)0(u)20.8(p)-317.9(wa)18.8(s)-317.7(r)0(e)19.7(p)0(l)20.7(a)0(c)18.5(e)0(d)-308.6(w)0(i)19.8(t)0(h)-307.6(o)0(s)21(t)0(e)19.6(o)0(g)20.8(e)0(n)19.7(i)0(c)-308.8(i)0(n)20.7(d)0(u)20.8(c)0(t)19.5(i)0(o)20.7(n)-317.9(me)18.4(-)]TJ
T*
[(di)14.9(um)19.6(,)-406.6(w)0(h)20(i)0(c)19.5(h)-410(co)13.9(ns)21(is)20.9(te)19.6(d)-410(o)0(f)-399.6(h)0(i)14.9(g)0(h)20.8(-)0(g)20.9(l)0(u)20.7(c)0(o)19.7(s)0(e)-400.6(D)0(M)19.1(E)0(M)-401.7(\()0(S)15.3(t)0(e)19.6(m)0(P)19.8(r)0(o)]TJ
/F8 1 Tf
7.3823 0 0 6.5614 290.6645 576.1132 Tm
()Tj
/F4 1 Tf
9.843 0 0 9.843 296.3905 571.7479 Tm
(,)Tj
-24.041 -1.1174 TD
[(gi)14.9(bc)19.7(o\))20.9(,)-193.5(1)0(0)20.8(%)-192.2(FB)20(S)-191(\()0(S)21(t)0(e)19.6(m)0(P)19.8(r)0(o)20.9(,)-193.5(gi)20.7(bc)19.7(o\))15.1(,)-187.7(1)0(0)-146.2(n)0(m)19.6(o)0(l)20.7(/)0(L)-187.8(d)0(e)19.7(x)0(a)19.7(m)0(e)18.4(t)0(h)20.7(a)0(s)19.9(o)0(n)20.8(e)]TJ
0 -1.1116 TD
[(\(S)15.3(ig)20.7(ma)18.4(-A)20(ld)20.7(ri)20.7(ch)19.7(\),)-327(0.)18.5(2)-156.6(m)0(m)18.4(o)0(l)20.7(/)0(L)-326.1(a)0(s)19.9(c)0(o)13.9(r)0(b)20.9(i)0(c)-326(a)0(c)18.5(i)0(d)-324.9(\()0(S)15.3(i)0(g)20.7(m)0(a)18.4(-)0(A)20(l)0(d)20.7(r)0(i)20.7(c)0(h)19.7(\))0(,)]TJ
T*
[(10)-146.2(mm)18.4(ol)20.7(/L)]TJ
/F11 1 Tf
5.1031 0 TD
(b)Tj
/F4 1 Tf
.5414 0 TD
[(-g)20.9(ly)20.7(co)19.7(l-)15(2-)20.9(di)20.7(os)21(od)20.8(iu)15(m)-762.6(p)0(h)20.8(o)0(s)21(p)0(h)20.8(a)0(t)19.5(e)-768.3(sa)19.9(lt)-757(hy)20.8(dr)20.9(at)19.5(e)]TJ
-5.6445 -1.1174 TD
[(\(S)15.3(ig)20.7(ma)18.4(-A)20(ld)20.7(ri)20.7(ch)19.7(\),)-574.7(an)19.7(d)-588.6(1)0(%)-573.5(p)0(e)19.7(n)0(i)20.7(c)0(i)19.5(l)0(l)20.6(i)0(n)20.7()0(s)15.3(t)0(r)20.7(e)0(p)19.7(t)0(o)20.7(m)0(y)19.6(c)0(i)19.5(n)-582.8(so)15.3(lu)20.7(ti)20.6(on)]TJ
0 -1.1116 TD
[(\(S)15.3(te)19.5(mP)19.8(ro)20.9(,)-377.8(g)0(i)14.9(b)0(c)19.7(o)0(\))-370.8([)0(1)20.9(4)0(])20.9(.)-383.6(Th)19.6(e)-382.4(c)0(o)19.7(n)0(t)20.7(r)0(o)20.9(l)-381.4(gr)20.9(ou)20.8(ps)-370.6(we)13(re)-371.9(cu)19.7(lt)20.6(ur)20.9(ed)-372(in)]TJ
T*
[(MS)20.1(CM)18.9(,)-251.1(w)0(h)20(i)0(c)13.8(h)-248.8(wa)18.8(s)-248.6(r)0(e)19.7(f)0(r)15.1(e)0(s)19.9(h)0(e)19.7(d)-248.8(ev)19.7(er)19.7(y)-248.8(3)-254.5(da)19.7(ys)21(.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 69.7323 494.9857 Tm
[(Adipogenic)-520(differentiation.)]TJ
/F4 1 Tf
9.843 0 0 9.843 178.1291 494.9857 Tm
[(Third-generation)-500.7(labial)-504.7(gland)]TJ
-12.0263 -1.1116 TD
[(stem)-571.2(cells)-566.6(from)-564.3(the)-563.3(SS)-566.3(and)-568.9(control)-562(groups)-570.8(and)-563.2(human)]TJ
T*
[(adipose-derived)-435.9(MSCs)-432.2(\(hASCs;)-429.8(No.)-427(11537;)-433(Sciencell)-429.5(Re-)]TJ
0 -1.1174 TD
[(search)-413(Laboratories\))-416.4(were)-410.7(used)-408.6(for)-406.4(adipogenic)-412.2(differentia-)]TJ
0 -1.1116 TD
[(tion)-286.9(experiment)-10.2(s.)-286.6(The)-287.9(cells)-290.1(were)-289.8(seeded)-286.4(into)-286.9(24-)-291.3(and)-286.7(6-well)]TJ
T*
[(plates.)-455.9(When)-451.3(compete)-456.1(cell)-450.5(conuence)-457.9(was)-454.4(achieved,)-454.8(adi-)]TJ
T*
[(pogenic)-459.4(induction)-455(was)-454.4(initiated)-456.4(by)-457.2(using)-454.7(an)-452.6(adipogenesis)]TJ
0 -1.1174 TD
[(differentiation)-325.8(kit)-320.3(\(StemPro,)-319.6(gibco\))-324.7(and)-321.2(noninduced)-318.7(control)]TJ
0 -1.1116 TD
[(groups)-248.3(were)-255.2(cultured)-247.5(in)-250(MSCM.)-246.7(After)-249.5(21)-249.9(days)-247.3(of)-249.8(cultivation)]TJ
T*
[(of)-284.4(the)-281.1(adipogenic)-285.5(induction)-282.2(groups,)-280.5(the)-286.8(cells)-284.4(were)-284(subjected)]TJ
0 -1.1174 TD
[(to)-417(Oil)-413.4(Red)-414.4(O)-410.9(staining)-419.1(and)-413.4(real-time)-418.1(polymerase)-418.8(chain)-411.1(re-)]TJ
0 -1.1116 TD
[(action)-337.5(\(PCR\))-335.7(detection.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 69.7323 363.5149 Tm
[(Induction)-584.1(of)-578.4(differentiation)-583.3(into)-575.1(salivary)-586.7(gland)-577(epithelioid-)]TJ
-1.1128 -1.2267 TD
[(like)-561(cells.)]TJ
/F4 1 Tf
9.843 0 0 9.843 105.5622 352.5164 Tm
[(Third-generation)-541(labial)-539.2(gland)-536.7(stem)-536.6(cells)-543.5(from)]TJ
-4.6538 -1.1116 TD
[(the)-373.2(SS)-370.5(and)-367.3(control)-372(groups)-369.2(and)-373.1(hBMSCs)-366.3(were)-376.2(subjected)-370.6(to)]TJ
T*
[(induction)-639.3(culture)-643.8(for)-636.8(differentiation)-642.6(into)-638.2(salivary)-639(gland)]TJ
T*
[(epithelioid-like)-459.7(cells.)-460.6(The)-454.9(cells)-457.1(were)-451.1(seeded)-459.2(into)-453.9(six-well)]TJ
0 -1.1174 TD
[(plates.)-542.3(After)-543.3(80%)-540(cellular)-548.3(conuence)-544.3(was)-540.8(achieved,)-541.2(the)]TJ
0 -1.1116 TD
[(induction)-334(was)-327.7(conducted.)-333.8(The)-328.2(rat)-332.9(submandibular)-334.7(gland)-329.3(cell)]TJ
T*
[(line)-449.3(\(SMG-C6;)-449.3(Department)-448.8(of)-445.7(Pathology)-448.9(and)-448(Physiology,)]TJ
0 -1.1174 TD
[(Peking)-560.7(University)-560.4(Medical)-565.3(Laboratory\))-562.8(culture)-557.4(solutions)]TJ
0 -1.1116 TD
[(were)-399.2(collected)-400.9(and)-396.1(ltered,)-393.7(and)-396.1(epithelial)-401.1(cell)-392.9(culture)-401.9(me-)]TJ
T*
[(dium)-432(\(Gibco\))-436(was)-431.4(added)-434.1(to)-428.5(the)-436.6(culture)-430.7(for)-429.5(differentiation)]TJ
0 -1.1174 TD
[(into)-453.9(salivary)-454.7(gland)-456.1(epithelioid-like)-454(cells.)-454.8(The)-454.9(cells)-451.4(of)-451.4(the)]TJ
0 -1.1116 TD
[(noninduced)-393.6(control)-395(groups)-386.5(were)-393.5(cultured)-391.5(in)-394(MSCM.)-390.7(Cells)]TJ
T*
[(were)-497.1(subjected)-491.5(to)-491.9(immunouorescence)-499.1(staining)-494(and)-494(real-)]TJ
0 -1.1174 TD
[(time)-335.4(PCR)-339.3(detection)-336.3(after)-336.2(7)-329.4(days)-333.7(of)-336.2(induction.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 69.7323 199.1054 Tm
[(Alkaline)-581.9(phosphatase)-572.5(staining.)]TJ
/F4 1 Tf
9.843 0 0 9.843 195.0803 199.1054 Tm
[(After)-560.5(7)-542.5(d)0(a)19.7(y)0(s)-531.9(o)0(f)-537.8(i)0(n)20.7(d)0(u)20.8(c)0(e)18.5(d)]TJ
-13.7484 -1.1116 TD
[(os)15.3(te)19.5(og)20.8(en)19.7(ic)-579.5(di)20.7(ff)20.9(er)19.7(en)19.7(ti)14.8(at)19.5(io)20.7(n,)-574.7(ea)18.5(ch)-579.3(gr)20.9(ou)20.8(p)-588.6(o)0(f)-578.1(s)0(t)20.9(e)0(m)-580.6(c)0(e)18.5(l)0(l)20.6(s)-588.4(wa)18.8(s)]TJ
T*
[(su)15.3(bj)20.7(ec)18.5(te)19.6(d)-340.9(t)0(o)-324.9(a)0(l)13.8(k)0(a)19.7(l)0(i)20.6(n)0(e)-325.9(p)0(h)15.1(o)0(s)21(p)0(h)20.8(a)0(t)19.5(a)0(s)19.9(e)-342.1(\(A)20(LP)19.8(\))-340.9(s)0(t)20.9(a)0(i)19.5(n)0(i)20.7(n)0(g)-330.5(b)0(y)-330.5(u)0(s)21(i)0(n)20.7(g)-340.9(a)]TJ
0 -1.1174 TD
[(co)19.7(mm)18.4(er)19.7(ci)13.8(al)-326(AL)18.8(P)-335(r)0(e)19.7(a)0(g)13.9(e)0(n)19.7(t)-335.3(ki)20.7(t)-335.3(\()0(C)14.1(W)13.9(BI)14.1(O,)-327.9(In)20.9(c.)23.1(,)-337.5(C)0(h)19.8(i)0(n)15(a)0(\))19.7(,)-337.5(ac)18.5(co)19.7(rd)20.9(-)]TJ
0 -1.1116 TD
[(in)15(g)-398.5(t)0(o)-388.2(t)0(h)20.7(e)-399.7(ma)18.4(nu)15.1(fa)19.7(ct)19.5(ur)20.9(er)19.7(s)-393.6(in)20.7(st)20.9(ru)20.9(ct)19.5(io)20.7(ns)15.3(.)-400.8(G)0(r)20(a)0(y)19.7(-)0(b)20.9(r)0(o)20.9(w)0(n)-389(s)0(t)15.1(a)0(i)19.6(n)0(i)20.7(n)0(g)]TJ
T*
[(wa)18.8(s)-323.4(c)0(o)13.9(n)0(s)21(i)0(d)20.7(e)0(r)19.7(e)0(d)-314.4(a)-324.8(po)20.8(si)20.9(ti)20.6(ve)-314.4(re)19.7(su)15.3(lt)20.6(.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 69.7323 133.3417 Tm
[(Alizarin)-288.6(red)-286.7(staining)-287.6(for)-288.3(mineralized)-295.4(nodules.)]TJ
/F4 1 Tf
9.843 0 0 9.843 244.4598 133.3417 Tm
[(After)-289.8(21)-295.9(days)]TJ
-18.7651 -1.1116 TD
[(of)-439.9(induced)-447.9(osteogenic)-440.8(differentiation,)-444.5(each)-445.6(group)-443.2(of)-439.9(stem)]TJ
T*
[(cells)-555.1(was)-552.3(subjected)-549.1(to)-549.5(alizarin)-554.1(red)-551.6(staining;)-552.8(200)]TJ
/F10 1 Tf
21.2418 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
.5495 Tc
[(L1)549.5(%)]TJ
-21.7947 -1.1174 TD
0 Tc
[(alizarin)-300.6(red)-298.2(solution)-302.7(was)-298.9(added)-295.8(to)-296.1(the)-298.3(culture)-298.2(wells,)-304.8(and)-292.4(the)]TJ
0 -1.1116 TD
[(cells)-416.8(were)-416.5(incubated)-416.8(at)-412.4(room)-414.5(temperature)-415.6(for)-417.9(30)-163.5(min.)-411.3(De-)]TJ
T*
[(ionized)-396.2(water)-388.9(was)-396.8(used)-391.3(to)-388.2(rinse)-392.5(the)-390.5(cells)-393.8(until)-391.8(nonspecic)]TJ
0 -1.1174 TD
[(stains)-410.8(were)-416.5(completely)-413.5(removed.)-412.1(Formation)-409.8(of)-411.1(red)-407.6(miner-)]TJ
0 -1.1116 TD
[(alized)-338.7(nodules)-337.1(was)-333.4(considered)-337(a)-336.3(positive)-332.7(result.)]TJ
26.5234 67.9184 TD
[(Alizarin)-450.1(red)-447.9(staining)-453.7(semi-quantitative)-457.1(method:)-448.1(1%)-452.5(ce-)]TJ
-1.0137 -1.1174 TD
[(tylpyridine)-296(solution)-297(was)-298.9(added)-295.8(into)-298.4(each)-295.9(stained)-298.1(hole.)-297.1(After)]TJ
0 -1.1116 TD
[(being)-554(completely)-557.5(dissolved,)-561.6(100)]TJ
/F10 1 Tf
14.0594 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(L)-555.2(o)0(f)-555.1(the)-557.5(solution)-556.2(was)]TJ
-14.6124 -1.1116 TD
[(added)-428.3(into)-430.9(a)-428.5(96-well)-436.2(plate,)-432(and)-430.7(the)-425.1(absorb)-7.5(ance)-428.4(was)-431.4(mea-)]TJ
T*
[(sured)-334.7(at)-331.8(490)-170.3(nm.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 320.8251 670.3369 Tm
[(Observation)-367.3(of)-369.8(adipogenic)-368.4(differentiation)-374.6(by)-368.2(using)-369.5(Red)-367.5(Oil)-370.3(O)]TJ
-1.1128 -1.2203 TD
(staining.)Tj
/F4 1 Tf
9.843 0 0 9.843 350.9857 659.3951 Tm
[(After)-370.5(a)-365.1(21-day)-370.6(adipogenic)-366.1(induction,)-372(the)-367.5(cells)-370.8(in)]TJ
-4.0779 -1.1116 TD
[(each)-290.1(group)-293.5(were)-295.6(subjected)-289.9(to)-290.3(Red)-293.5(Oil)-292.4(O)-289.9(staining)-292.4(to)-290.3(observ)-7.5(e)]TJ
0 -1.1174 TD
[(cellular)-243(morphology)-248.5(and)-240.6(red)-246.3(adipose)-240.4(droplets.)-249.6(After)-243.7(removal)]TJ
0 -1.1116 TD
[(of)-238.3(the)-240.7(culture)-246.4(medium)-240.7(from)-241.7(the)-240.7(culture)-246.4(dishes,)-241.3(the)-240.7(cells)-244(were)]TJ
T*
[(rinsed)-428.1(once)-433(with)-426(PBS)-430.2(and)-430.7(xed)-425.9(with)-431.8(10%)-430.6(formaldehyde-)]TJ
0 -1.1174 TD
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T*
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T*
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0 -1.1174 TD
[(seconds)-320.7(of)-313.2(differentiation)-320.1(with)-322.3(70%)-315.4(ethanol.)-319(After)-318.6(the)-315.6(cells)]TJ
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T*
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T*
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[(\)i)329.4(n)]TJ
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0 Tc
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T*
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T*
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[(\).)-499.8(After)-502.9(the)-505.7(cells)-503.2(were)]TJ
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[(rinsed)-307.2(with)-305(PBS)-309.3(solution,)-306.2(xed)-310.7(with)-305(4%)-308.5(paraformaldehyde,)]TJ
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12.4525 0 TD
()Tj
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[(X-100,)-476.3(3%)-469.8(hydrogen)-476.5(per-)]TJ
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4.2391 0 TD
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[(,)-308.7(and)]TJ
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()Tj
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[(C,)-454.8(re-)]TJ
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T*
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T*
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[(the)-471.1(dark.)-475.5(The)-472.2(protein)-475.6(expression)-479.6(differences)-477.4(among)-474.4(each)]TJ
T*
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1.0137 -1.1174 TD
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T*
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14.8525 0 TD
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[(,)-413.9(AMY1,)-418.8(AQP5,)]TJ
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[(and)-333.6(ZO-1)-331.3(mRNA)-334.5(expression)-338.7(with)-333.3(real-time)-342.1(PCR.)]TJ
/F4 1 Tf
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[(After)-335.9(7)-335.2(days)]TJ
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0 -1.1116 TD
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T*
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T*
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2.5112 0 TD
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[(\))-496.4(was)-500.5(detected)-503.3(by)-497.5(using)-495(real-time)]TJ
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T*
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(AQP5)Tj
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(ZO-1)Tj
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[(\))-300.6(typically)-312.3(expressed)-306.7(in)-301.8(the)-309.9(salivary)-304.9(glands)]TJ
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T*
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T*
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[(sequences)-526.8(are)-524(listed)-526.3(in)-526.5(Table)-527.5(1)]TJ
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(b)Tj
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[(-actin)-527.5(was)-529.3(used)-523.7(as)-527.3(an)]TJ
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[(LABIAL)-339.4(GLAND)-333.3(MSC)]TJ
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(S)Tj
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[(OF)-331.2(HEALTHY)-338(SUBJECTS)-334.7(AND)-337.2(SS)-337.3(PATIENTS)-20007.2(1173)]TJ
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endstream
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[(Data)-255.4(were)-255.2(expressed)-260.7(as)-256.6(the)-252.3(mean)]TJ
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[(standard)-258.7(deviation)-254.5(and)]TJ
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[(were)-508.7(analyzed)-508.9(by)-509.1(using)-506.5(SPSS)-504.9(17.0)-507.8(software.)-504.9(Two-tailed)]TJ
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T*
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T*
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[(uantica)-5.1(tion,)-415.2(a)-5.1(n)1.8(d)-418.6(r)1.9(elative)]TJ
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[(ered)-340.8(statistically)-335.1(signicant.)]TJ
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[(Pr)-8.2(imary)-335.3(cu)-6.4(lture)-334.5(of)-339.2(labial)-336.1(gland)-339.6(stem)-337.8(cells)]TJ
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[(Stem)-340.8(cell-like)-336.4(cells)-342(and)-332.8(a)-336.3(few)-339.4(epithelium-like)-338.8(cells)-342(grew)]TJ
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[(as)-268.1(a)-261.4(monolayer)-267(from)-264.8(around)-266.9(the)-263.8(tissue)-266.8(block)-260.2(after)-267.1(a)-267.2(5-day)-260.1(in)]TJ
T*
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[(and)-269.4(control)-268.3(groups.)-269(Primary)-269.2(cells)-267.1(proliferated)-276.2(rapidly)-268.3(after)-267.1(a)]TJ
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[(7-day)-473.2(in)-474.6(vitro)-472.3(cultivation.)-473.5(Figure)-474.2(1A)-469.6(shows)-472.5(the)-471.1(complex)]TJ
T*
[(cellular)-323.7(array)-324.6(containing)-319(spindle-shap)-9.6(ed)-320.1(stem)-317.7(cell-like)-324.9(cells)]TJ
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[(and)-436.4(polygonal)-437.6(epithelium-like)-442.5(cells)-434.1(in)-434.3(the)-436.6(primary)-436.4(mono-)]TJ
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[(layer)-399.6(culture.)-399.6(The)-397.3(P1)-393.7(cells,)-403(which)-393.6(were)-399.2(obtained)-397.3(by)-399.6(long-)]TJ
T*
[(time)-513.9(enzymolysi)-8.9(s,)-511.2(were)-514.4(all)-511.6(spindle-shaped)-519.9(stem)-513.6(cell-like)]TJ
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[(cells)-560.8(\(Fig.)-554.9(1B\).)-560.7(The)-558.6(cellular)-559.8(morphology)-553.8(in)-555.3(the)-557.5(normal)]TJ
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[(control)-268.3(group)-270.4(was)-264.3(not)-268.4(signicantly)-269.1(different)-271.6(from)-264.8(that)-270.8(in)-267.3(the)]TJ
ET
62.022 719.49 490.11 .22668 re
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62.022 611.886 490.11 .22675 re
f
62.022 700.384 490.11 .22681 re
f
BT
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(Table)Tj
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(1.)Tj
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[(Sequences)-333.2(of)-337(Primers)-336(Used)-332.7(for)-332.2(Real-Time)-334.5(Polymerase)-338.2(Chain)-330.8(Reaction)]TJ
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[(Genes)-11628.7(Forward)-337(primer)-336(\(5)]TJ
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[(\))-13919.4(Reverse)-334.9(primer)-336(\(5)]TJ
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(RUNX-2)Tj
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[(CGCATTCCTCATCCCAGTAT)-10025(AGGGGTAAGACTGGTCATAGGA)]TJ
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[(CTGTATCAATGGCTGGGAGC)-9713(GCCTGGAGAGGAGCAGAACT)]TJ
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(a)Tj
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[(GTTTTGCTCGGATACTTGCCA)-9359(CCCCCAGGATCAAAAGTAATC)]TJ
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(c)Tj
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[(GGAATTAGATGACAGCGACTTGG)-7507(GGCTTGTAGCAGGTTGTCTTGAA)]TJ
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[(CCATTCACAACCCTTTCAGAC)-9255(CACATAAATACGAACCCCAACA)]TJ
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