ࡱ> 8:7q` RbjbjqPqP2&::$ 1*$[h   V  " I T.01$h JV < l1  Title:Role of histone deacetylation in cell-specific expression of endothelial nitric-oxide synthase Author(s): HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=OneClickSearch&field=AU&val=Gan+YH&curr_doc=2/2&Form=FullRecordPage&doc=2/2" \o "one-click search" Gan YH,  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=OneClickSearch&field=AU&val=Shen+YH&curr_doc=2/2&Form=FullRecordPage&doc=2/2" \o "one-click search" Shen YH,  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=OneClickSearch&field=AU&val=Wang+J&curr_doc=2/2&Form=FullRecordPage&doc=2/2" \o "one-click search" Wang J,  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=OneClickSearch&field=AU&val=Wang+XW&curr_doc=2/2&Form=FullRecordPage&doc=2/2" \o "one-click search" Wang XW,  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=OneClickSearch&field=AU&val=Utama+B&curr_doc=2/2&Form=FullRecordPage&doc=2/2" \o "one-click search" Utama B,  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=OneClickSearch&field=AU&val=Wang+J&curr_doc=2/2&Form=FullRecordPage&doc=2/2" \o "one-click search" Wang J,  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=OneClickSearch&field=AU&val=Wang+XL&curr_doc=2/2&Form=FullRecordPage&doc=2/2" \o "one-click search" Wang XL Source:JOURNAL OF BIOLOGICAL CHEMISTRY 280 (16): 16467-16475 APR 22 2005 Document Type:Article Language:English  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=PagedCitedRefList&UT=000228444800121&doc=2/2&rec_id=143916086" Cited References: 31  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Z42gbC@C47B3DmNLDND&Func=DispCitingRec&doc=2/2&isickref=143916086" Times Cited: 1 HYPERLINK "Gan%20Yehua2005.pdf" INCLUDEPICTURE "http://images.isiknowledge.com/WoK3/Images/Links/WOK3/Generic_Src.gif" \* MERGEFORMATINET  Abstract:Histone acetylation plays an important role in chromatin remodeling and gene expression. The molecular mechanisms involved in cell-specific expression of endothelial nitric-oxide synthase ( eNOS) are not fully understood. In this study we investigated whether histone deacetylation was involved in repression of eNOS expression in non-endothelial cells. Induction of eNOS expression by histone deacetylase ( HDAC) inhibitors trichostatin A (TSA) and sodium butyrate was observed in all four different types of non-endothelial cells examined. Chromatin immunoprecipitation assays showed that the induction of eNOS expression by TSA was accompanied by a remarkable increase of acetylation of histone H3 associated with the eNOS 5'-flanking region in the non-endothelial cells. Moreover, DNA methylation-mediated repression of eNOS promoter activity was partially reversed by TSA treatment, and combined treatment of TSA and 5-aza-2'-deoxycytidine (AzadC) synergistically induced eNOS expression in non-endothelial cells. The proximal Sp1 site is critical for basal activity of eNOS promoter. The induction of eNOS by inhibition of HDACs in non-endothelial cells, however, appeared not mediated by the changes in Sp1 DNA binding activity. We further showed that Sp1 bound to the endogenous eNOS promoter and associated with HDAC1 in non-endothelial HeLa cells. Combined TSA and AzadC treatment increased Sp1 binding to the endogenous eNOS promoter but decreased the association between HDAC1 and Sp1 in HeLa cells. Our data suggest that HDAC1 plays a critical role in eNOS repression, and the proximal Sp1 site may serve a key target for HDCA1-mediated eNOS repression in non-endothelial cells. KeyWords Plus:REPRESS TRANSCRIPTION; GENE-EXPRESSION; DNA METHYLATION; BINDING PROTEIN; SP1 SITE; PROMOTER; INHIBITORS; ACETYLATION; COMPLEX; HTERT Addresses:Wang XL (reprint author), Baylor Coll Med, Michael E DeBakey Dept Surg, Div Cardiothorac Surg, MS NAB 2010, Houston, TX 77030 USA Baylor Coll Med, Michael E DeBakey Dept Surg, Div Cardiothorac Surg, Houston, TX 77030 USA Peking Univ, Sch Stomatol, Ctr TMJ Disorders, Beijing, 100081 Peoples R China E-mail Addresses: HYPERLINK "mailto:xlwang@bcm.tmc.edu" xlwang@bcm.tmc.edu Publisher:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA Subject Category:BIOCHEMISTRY & MOLECULAR BIOLOGY IDS Number:917GO ISSN:0021-9258 egqrs% & , - / 0 _ ` g h j k   & ' ) * жККККiККiККiККiККi,hShS>*B*CJKHOJQJ^Jph2hShS5>*B*CJKHOJQJ\^Jph6jhShSB*CJKHOJQJU^JaJph3hShS5B*CJKHOJQJ\^JaJph-hShSB*CJKHOJQJ^JaJph/hShS5B*CJKHOJQJ\^Jph)g 7n(\o $1$a$gdS $1$9Da$gdS @AMOPUf̵iiS*h@B*CJKHOJQJ^JaJo(ph2hShS5>*B*CJKHOJQJ\^Jph3hShS5B*CJKHOJQJ\^JaJph/hShS5B*CJKHOJQJ\^Jph,hShS>*B*CJKHOJQJ^Jph6jhShSB*CJKHOJQJU^JaJph-hShSB*CJKHOJQJ^JaJphfg7An︨yayayayayEyE6jhShSB*CJKHOJQJU^JaJph/hShS5B*CJKHOJQJ\^Jph-hShSB*CJKHOJQJ^JaJph.jhrh@B*CJOJQJUaJphh@B*CJOJQJaJph(jh@B*CJOJQJUaJph+jh~h~CJOJQJUaJh~CJOJQJaJjh@CJOJQJUaJ(9\got~Ͷ hSo(/hShS5B*CJKHOJQJ\^Jph-hShSB*CJKHOJQJ^JaJph6jhShSB*CJKHOJQJU^JaJph,hShS>*B*CJKHOJQJ^Jph 0182P. 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