ࡱ> 574q` RbjbjqPqP2 ::     ( =*@ghB   N   4 ` N .  0= | X ,Z @ \ 4 p =  Title:A pilot study of fatty acid metabolism in oral squamous cell carcinoma Author(s): HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Y6D1KGgC3Nkf68d5iEe&Func=OneClickSearch&field=AU&val=Zhang+Y&curr_doc=3/1&Form=FullRecordPage&doc=3/1" \o "one-click search" Zhang Y,  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Y6D1KGgC3Nkf68d5iEe&Func=OneClickSearch&field=AU&val=Guo+C&curr_doc=3/1&Form=FullRecordPage&doc=3/1" \o "one-click search" Guo C,  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Y6D1KGgC3Nkf68d5iEe&Func=OneClickSearch&field=AU&val=Yu+G&curr_doc=3/1&Form=FullRecordPage&doc=3/1" \o "one-click search" Yu G Source:INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY 34 (1): 78-81 JAN 2005 Document Type:Article Language:English  HYPERLINK "http://wos02.isiknowledge.com/CIW.cgi?SID=Y6D1KGgC3Nkf68d5iEe&Func=PagedCitedRefList&UT=000226402100012&doc=3/1&rec_id=142563459" Cited References: 26 Times Cited: 0 HYPERLINK "Y.Zhang2005.pdf" \t "_blank" INCLUDEPICTURE "http://images.isiknowledge.com/WoK3/Images/Links/WOK3/Generic_Src.gif" \* MERGEFORMATINET  Abstract:This study was undertaken to determine the characteristics of fatty acid synthesis in squamous carcinoma cells and to assess the possibility of exploiting this synthesis pathway in anticancer therapy. Incorporation of [H-3]acetate into total lipids of the TCA-83 squamous carcinoma cell line and normal gingival fibroblasts was measured. The effects of cerulenin, a specific, potent, noncompetitive inhibitor of fatty acid synthase (FAS), on growth of TCA-83 cells and normal gingival fibroblasts was determined by the MTT method, and the effect of cerulenin on apoptosis was determined by electrophoresis of cellular DNA. Incorporation of [H-3]acetate into total lipids was significantly higher in TCA-83 cells (68 +/- 12.7 cpm per 2 x 10(5) cells) than in normal gingival fibroblasts (13 +/- 4.2 cpm per 2 x 10(5) cells; P = 0.011). Cerulenin inhibited TCA-83 cell growth in a dose-dependent manner, even at 2.5 mug/ml, a concentration at which normal gingival fibroblasts were not inhibited. After exposure to cerulenin, TCA-83 cells showed typical apoptotic DNA ladders. These results indicate that cerulenin exerts selective cytotoxic effects on squamous carcinoma cells and can induce their apoptosis. These findings suggest that targeting of fatty acid synthesis may be useful in the treatment of squamous cell carcinomas. Author Keywords:fatty acid synthesis; squamous cell; carcinoma; cerulenin; apoptosis KeyWords Plus:BREAST-CANCER CELLS; SYNTHASE EXPRESSION; PROSTATE-CANCER; INHIBITION; TUMOR; PROGRESSION; SYNTHETASE; PREDICTOR; XENOGRAFT; CERULENIN Addresses:Zhang Y (reprint author), Peking Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, 22 S Zhongguancun Ave,Haidian Dist, Beijing, 100081 Peoples R China Peking Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Beijing, 100081 Peoples R China E-mail Addresses: HYPERLINK "mailto:zhangyie1101@sohu.com" zhangyie1101@sohu.com Publisher:CHURCHILL LIVINGSTONE, JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND Subject Category:DENTISTRY, ORAL SURGERY & MEDICINE; SURGERY IDS Number:888VK ISSN:0901-5027 MOYZ[         жККККiККiжККiж,hErhEr>*B*CJKHOJQJ^Jph2hErhEr5>*B*CJKHOJQJ\^Jph6jhErhErB*CJKHOJQJU^JaJph3hErhEr5B*CJKHOJQJ\^JaJph-hErhErB*CJKHOJQJ^JaJph/hErhEr5B*CJKHOJQJ\^Jph)O  gdEr $1$a$gdEr $1$9Da$gdEr w x y z { (ŪՕmV>V>V>V>V>V/hErhEr5B*CJKHOJQJ\^Jph-hErhErB*CJKHOJQJ^JaJph.jhrh B*CJOJQJUaJphh B*CJOJQJaJph(jh B*CJOJQJUaJph4jhhB*CJOJQJUaJphhB*CJOJQJaJph(jhDoB*CJOJQJUaJph*h B*CJKHOJQJ^JaJo(ph͖͞͞͞͞hErhxo(/hErhEr5B*CJKHOJQJ\^Jph,hErhEr>*B*CJKHOJQJ^Jph-hErhErB*CJKHOJQJ^JaJph6jhErhErB*CJKHOJQJU^JaJph0182P. A!"#$%S DyK _blankF YZHANG~1.PDF$Y.Zhang2005.pdfDdJO]  c ADGeneric_SrcView full text from the publisherbzI`cW=fD!inzI`cW=fD!iPNG  IHDRNYZPLTE3ҏ-VWr|߹ֺ=ײ?erJѬտȏUp۵U cmPPJCmp0712HsIDAT8OUR0]UJKVh "xAxrD >wM7ɞg&\$ k(pQn+7%FE!#PO0t#E<Ձ[_DNܹ:\ G[:aR[p!賒|0MAm*OJQJo(ph@@ x fieldlabel15OJQJ\o(  O     0000000000000000O     @0@0@0@0@0@0@0@0@0@0@0@0@0@0@0 0     Z  wyz   XXXXXCXgs̨h gsDh gsz` gsj gs` gs,h Z Z y  k k  :*urn:schemas-microsoft-com:office:smarttagsStreet;*urn:schemas-microsoft-com:office:smarttagsaddress9*urn:schemas-microsoft-com:office:smarttagsplace8*urn:schemas-microsoft-com:office:smarttagsCityB*urn:schemas-microsoft-com:office:smarttagscountry-region (? 6>7?eh     "   " # . \ ` b e f n  nvq 333  {  -K&8/ K&8/0*P0K&8/CA!K&8/PE$P0hm$P0lN%P0^*)K&8/K&8/P0q2P03K&8/s <4K&8/>;K&8/B?P0R?K&8//^lBP0~+CK&8/TK&8/4cTP0d0XK&8/IYP0\P0jA`K&8/;gK&8/e.ehP0XiP0