аЯрЁБс>ўџ 68ўџџџ5џџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџьЅСq` №RП‚bjbjqPqP2::‚џџџџџџЄ№№№№№№№ŒŒŒŒ  ы*И^jllllll$h}*№. . . №№Ѕˆ ˆ ˆ . 6№№jˆ . jˆ ˆ №№ˆ Ќ РхЉэ_ѕХŒd ˆ jЛ0ыˆ Їn Їˆ ˆ ~ Ї№dLb6ˆ ˜,Фj~ ы. . . . ФШФШ№№№№№№џџџџ Title: Kinetic and site-directed mutagenesis studies of Prevotella intermedia acid phosphatase Author(s):  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Y6lGeO7opkg2OKkf4e3&Func=OneClickSearch&field=AU&val=Chen+XC&curr_doc=13/1&Form=FullRecordPage&doc=13/1" \o "one-click search" Chen XC,  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Y6lGeO7opkg2OKkf4e3&Func=OneClickSearch&field=AU&val=Ansai+T&curr_doc=13/1&Form=FullRecordPage&doc=13/1" \o "one-click search" Ansai T,  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Y6lGeO7opkg2OKkf4e3&Func=OneClickSearch&field=AU&val=Barik+S&curr_doc=13/1&Form=FullRecordPage&doc=13/1" \o "one-click search" Barik S,  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Y6lGeO7opkg2OKkf4e3&Func=OneClickSearch&field=AU&val=Takehara+T&curr_doc=13/1&Form=FullRecordPage&doc=13/1" \o "one-click search" Takehara T Source: PROTEIN AND PEPTIDE LETTERS 10 (1): 53-59 FEB 2003 Document Type: Article Language: English  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Y6lGeO7opkg2OKkf4e3&Func=PagedCitedRefList&UT=000181186600007&doc=13/1&rec_id=128495143" Cited References: 31      Times Cited: 0      Abstract: Site-directed mutagenesis was used to examine the roles of the conserved histidine, arginine and cysteine residues in acid phosphatase from Prevotella intermedia (PiACP). The replacement of histidine and arginine residues resulted in the elimination of the PiACP activity while the cysteine mutants retained activity. These results suggest that the histidine and arginine residues are essential for catalysis. KeyWords Plus: PHOSPHOTYROSYL PHOSPHATASE; CURVULARIA-INAEQUALIS; ACTIVE-SITE; GENE; IDENTIFICATION; SEQUENCE; CLONING; GLUCOSE-6-PHOSPHATASE; CHLOROPEROXIDASE; EXPRESSION Addresses: Kyushu Dent Coll, Dept Prevent Dent, Kitakyushu, Fukuoka 8038580 Japan Peking Univ, Sch Stomatol, Dept Prevent Dent, Beijing, 100081 Peoples R China Univ S Alabama, Coll Med, Dept Biochem & Mol Biol, Mobile, AL 36688 USA Publisher: BENTHAM SCIENCE PUBL LTD, PO BOX 1673, 1200 BR HILVERSUM, NETHERLANDS Subject Category: BIOCHEMISTRY & MOLECULAR BIOLOGY IDS Number: 649BG ISSN: 0929-8665 ^`jkl! " ) * , - т у ъ ы э ю Ѓ Є Ћ Ќ Ў Џ g h r s u | } ˜ Б П Щ в м н l m чаЖачаšаš€šаšаšišаšаšišаšаšišачаЖачачаšаš,h)ѕh)ѕ>*B*CJKHOJQJ^Jphџ2h)ѕh)ѕ5>*B*CJKHOJQJ\^Jphџ6jh)ѕh)ѕB*CJKHOJQJU^JaJph3h)ѕh)ѕ5B*CJKHOJQJ\^JaJph-h)ѕh)ѕB*CJKHOJQJ^JaJph/h)ѕh)ѕ5B*CJKHOJQJ\^Jph)`u Б Щ м  Bяи*^q‚єєєєєыєєєєєєєцgd)ѕ $1$a$gd)ѕ $1$9Da$gd)ѕ‚§m ~  ‚ ˆ ”  І BPяљит*;^iqv‚цЯГœ‚œjœjœjœjœjœjœjœbh)ѕhй Чo(/h)ѕh)ѕ5B*CJKHOJQJ\^Jph3h)ѕh)ѕ5B*CJKHOJQJ\^JaJph-h)ѕh)ѕB*CJKHOJQJ^JaJph6jh)ѕh)ѕB*CJKHOJQJU^JaJph,h)ѕh)ѕ>*B*CJKHOJQJ^Jphџ2h)ѕh)ѕ5>*B*CJKHOJQJ\^Jphџ0182PА‚. АЦA!А"А# $ %ААSАр Љ†œN@ёџN ck‡e $1$a$$CJKHPJ_HaJmH nHsH tH$A@ђџЁ$ иžЄ‹Еk=„W[SOBi@ѓџГB nfhˆ*OJQJo(phџ@ўoЂ@ ФШ fieldlabel15OJQJ\o(‚џџџџ`uБЩмBяи*^q„˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€˜0€€`uБЩмBяи*^q„KШ0Р§KШ0Р§KШ0Р§KШ0Р§KШ0Р§KШ0Р§KШ0Р§KШ0Р§KШ0Р§KШ0Р§IШ0Р€IШ0Р€IШ0Р€ 0ym ‚ ‚ ‚ k!),тъэЃЋЎgrмl‚XџŒXџŒXџŒXџŒXџŒџџЯY RT аY,Г] бY”ьN вYєh гYD„((„B*€urn:schemas-microsoft-com:office:smarttags€country-region€8*€urn:schemas-microsoft-com:office:smarttags€City€:*€urn:schemas-microsoft-com:office:smarttags€Street€;*€urn:schemas-microsoft-com:office:smarttags€address€9*€urn:schemas-microsoft-com:office:smarttags€place€ p8%8BCMS^№љћ"-3=>HJOens{Ј­СЩ BJ HLNQRZ“ŸЃЎЕМР„„„„ЮM#F&C4@#F&7{ŽTYЈ){б?ЧKЎ р‰ З]aB рyFВ'wf‚vы#F&•6.m/йFIP‚v-'{б?ApВ'wfS" р(і‰ ЗVnі‚v%D_,?‘/Ёm/йF+n№‚vd(ШŒa!kдВ'wf uхSQYHЈ ‚v$dХ  рЅљŒaГц$<ЧbГXtt`?AЕm/йFQчВ'wf*\В'wfHqПtt`?m {б?‹+dŒa(?š$<Чbнbf$<ЧbTYZ\•TY/ђ‰ ЗuPХŒa-Jk,?нŒВ'wfяzdm/йF†=-‰ З}%7 рГj В'wfEЇm/йFpсtt`? 5D‚vЁ7”#F&щvКВ'wf`Њ‰ Зи>Š,?NАm/йF рl tt`?‡jy tt`?ъ9Ч TY†!‰ З% Ъ!#F&vА"_Н/)/A#ŒaWѕ# ро. $Œaљf${б?Ž$_Н/#F&T}+_Н/‡ю+SQY V,SQYЖQC,Œa3wN,В'wfњ<,,?*nЖ-$<Чb).SQYТъ.SQYŸ(/#F&_Н/yaч/$<Чb>g0$<ЧbSdд0_Н/В&н1SQY'^2_Н/y,-2SQY‚ю2{б?шŸ3 рycЈ3TY7<Д3В'wfH^Ѕ4#F&Рit5 р§yЛ5{б? "9TYХ7i9В'wfB9TY1?И;_Н/x b= рТz=m/йFЋ'5>TYyX>SQY}Qx>#F&‹t4?{б?tt`?,?{б?/Nк?tt`?Ѕ/@TYЁJf@SQY-pA‚vлаAŒaбiЈB_Н/r-ФBŒaРlйDSQYj E$<ЧbсETYлџETYzF,?KЦF рm/йFЦtJ$<Чb5X:J,?ЎBЅKSQY'TђK,?LВ'wf*M‰ ЗИ]0M#F&(QvN‰ З AƒN‰ Зє9_Om/йF*H$P рzdЋP рmCШPSQYˆ=ЯQ‰ ЗБ2уQ‚vg