ࡱ> ;=:q` RbjbjqPqP2*:: l*x x x x x x x $h$e x x e e x x &e Jx x e x  .<0l"""x B  tF x x x  x x x le e e e DHDH Title:Polymorphonuclear neutrophils and their mediators in gingival tissues from generalized aggressive periodontitis Author(s): HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=OneClickSearch&field=AU&val=Liu+RK&curr_doc=18/1&Form=FullRecordPage&doc=18/1" \o "one-click search" Liu RK,  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=OneClickSearch&field=AU&val=Cao+CF&curr_doc=18/1&Form=FullRecordPage&doc=18/1" \o "one-click search" Cao CF,  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=OneClickSearch&field=AU&val=Meng+HX&curr_doc=18/1&Form=FullRecordPage&doc=18/1" \o "one-click search" Meng HX,  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=OneClickSearch&field=AU&val=Gao+Y&curr_doc=18/1&Form=FullRecordPage&doc=18/1" \o "one-click search" Gao Y Source:JOURNAL OF PERIODONTOLOGY 72 (11): 1545-1553 NOV 2001 Document Type:Article Language:English  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=PagedCitedRefList&UT=000172411700011&doc=18/1&rec_id=122554589" Cited References: 47  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=DispCitingRec&doc=18/1&isickref=122554589" Times Cited: 10  HYPERLINK "http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=EasyRelated&UT=000172411700011&doc=18/1&rec_id=122554589"  INCLUDEPICTURE "http://images.isiknowledge.com/WoK3/Images/relrec.gif" \* MERGEFORMATINET  HYPERLINK ""  INCLUDEPICTURE "http://images.isiknowledge.com/WoK3/Images/info.gif" \* MERGEFORMATINET  Abstract:Background: Impaired polymorphonuclear neutrophil (PMN) functions were generally considered to be related to the onset of generalized aggressive periodontitis (GAgP). However, some research has indicated that the hyperreactivity of PMN seems to be involved in the inflammatory response of GAgP. The present study's main purpose was to provide more evidence about the role of PMN in the pathogenesis of GAgP by surveying PMN infiltration in gingiva and its relationship with the expression of their mediators including intercellular adhesion molecule-1 (ICAM-1), interleukin-8 (IL-8), interleukin-1 beta (IL- 1 beta), and tumor necrosis factor-alpha (TNF-alpha). The inflammatory response in GAgP was also compared with that in adult periodontitis (AP) and periodontally healthy subjects. Since these PMN mediators were reported to be produced mainly by macrophages, the association between the expression of these PMN mediators and the distribution of macrophages was also investigated. Methods: A total of 25 gingival specimens were obtained from 10 GAgP patients, 10 AP patients, and 5 periodontally healthy subjects. Serial sections were obtained from each specimen, and the following techniques were adopted to investigate the distribution and interrelation of different cells and cytokines. Infiltration of PMN was observed by using hematoxylin and eosin staining. Distribution of the macrophages, identified as CD68(+), was shown by using immunohistochemistry. Immunohistochemistry and in situ hybridization were used to detect the expression of ICAM-1, IL-8, IL-1 beta, and TNF-alpha in gingival tissues. These techniques were performed in serial sections from each individual specimen. Results: Large numbers of infiltrating PMNs were observed in gingiva from GAgP. In gingiva from both GAgP and AP, the strongest protein and mRNA expression of IL-8, ICAM-1, IL-1 beta, and TNF-alpha were located in pocket epithelium and adjacent connective tissue with large numbers of infiltrating PMNs. In tissues without abundant PMN infiltration, the appearance of positive cells expressing IL-8, ICAM-1, IL-1 beta, and TNF-alpha was scattered. CD68(+) was distributed sparsely in connective tissue and was hardly seen in pocket epithelium with large numbers of PMN infiltration. The degree of leukocyte infiltration and connective tissue destruction in gingiva from GAgP patients was not distinctly different from that in gingiva from AP. The gingival specimens with heavy PMN infiltration from both GAgP and AP patients presented strong expressions of IL-1 beta and TNF-alpha; showed more extensive inflammatory cell infiltration; had severe connective tissue destruction; and presented severe elongation and ulceration of pocket epithelium. In gingiva from healthy subjects, inflammation was minor with visually no PMN, CD68(+), or the positive cells of IL-8, ICAM-1, IL-1 beta and TNF-alpha expression. Conclusions: Enhanced accumulation of PMN, which is associated with the upregulation of IL-8, ICAM-1, IL-1 beta, and TNF-alpha expression, relates to the severity and activity of GAgP. In addition to macrophages, PMN and/or epithelial cells might also be important sources of IL-8, IL-1 beta, and TNF-alpha production in gingiva. Author Keywords:neutrophils, polymorphonuclear; intercellular adhesion molecule-1; interleukin-8; interleukin-1; tumor necrosis factor; periodontitis; generalized aggressive/pathogenesis KeyWords Plus:RAPIDLY PROGRESSIVE PERIODONTITIS; EARLY-ONSET PERIODONTITIS; ORAL EPITHELIAL-CELLS; NECROSIS-FACTOR-ALPHA; CREVICULAR FLUID; MESSENGER-RNA; ADULT PERIODONTITIS; BETA-GLUCURONIDASE; CYTOKINES; INTERLEUKIN-8 Addresses:Liu RK (reprint author), Peking Univ, Dept Periodontol, Sch Stomatol, 38 BaiShiQiao Rd, Beijing, 100081 Peoples R China Peking Univ, Dept Periodontol, Sch Stomatol, Beijing, 100081 Peoples R China Peking Univ, Dept Oral Pathol, Beijing, 100081 Peoples R China Publisher:AMER ACAD PERIODONTOLOGY, 737 NORTH MICHIGAN AVENUE, SUITE 800, CHICAGO, IL 60611-2690 USA Subject Category:DENTISTRY, ORAL SURGERY & MEDICINE IDS Number:496TC ISSN:0022-3492 vx8 9 ? @ B C   v w | }  y z жККККiККiККiжКК,hDhD>*B*CJKHOJQJ^Jph2hDhD5>*B*CJKHOJQJ\^Jph6jhDhDB*CJKHOJQJU^JaJph3hDhD5B*CJKHOJQJ\^JaJph-hDhDB*CJKHOJQJ^JaJph/hDhD5B*CJKHOJQJ\^Jph)x i,0Cy$K-1$9Da$gdD $1$a$gdD $1$9Da$gdDz    ! ' ( "#$}~ϳϳiiM6jhDhDB*CJKHOJQJU^JaJph-hDhDB*CJKHOJQJ^JaJph6jhDhDB*CJKHOJQJU^JaJph-hDhDB*CJKHOJQJ^JaJph6jhDhDB*CJKHOJQJU^JaJph,hDhD>*B*CJKHOJQJ^Jph2hDhD5>*B*CJKHOJQJ\^Jph0@CTy hDo(-hDhDB*CJKHOJQJ^JaJph/hDhD5B*CJKHOJQJ\^Jph0182P. A!"#$%S DdJ  s rA*relrecbrelrecFind Related RecordsbA2w@Q}L]QD* nA2w@Q}L]QPNG  IHDRo62[PLTE333գTTQøfff===𴴮``]JJIxxtͽߖfff@@@WWVppnΥźrrr||{YYY,Nh/tRNSZ8bKGDHgIFg>U cmPPJCmp0712HsIDATHKVђ0 _ 4aΐwچdtL-V^+q(c(,mu[_ۗnJ"**Jxv"gS@4:̹aM+NŬqӪK#%zOJ)"%QҐq‡뱫|oFQ3:K Rb?5}<PH:*,֨ZKUaImSF<>Ө- |IBNϡN'ixEo@~'$vmԅl+5)MTX,%D7ai.FEݜY7Wzv8YÞWZ"{1>|i{>*e8?h DBe> 9MUV%{ 86-R(tA2θp=`nj!JVsd kdO\?wꗵ |qz~%j-mxoi#~7]_QDIENDB`Ddj  c FA infoInformationb70л"[* n 0л"[PNG  IHDR(PLTE333պde^GGGxxqݙqqjԅSSSƺ̣zzz噙WWRtttfffxFa +tRNS#ɧbKGDHgIFg>U cmPPJCmp0712HsIDATWU0 ;'ÁlL`}-?MܗkzsJ'6)3Z[TMLW3A)GiEv A?WvFw\@/!.sק w.Unoqգ|?Oݲ_P_lSIENDB`N@N cke $1$a$$CJKHPJ_HaJmH nHsH tH$A@$ ؞k=W[SOBi@B nfh*OJQJo(ph@o@ D fieldlabel15OJQJ\o(*xi , 0CyI0I0I0I0I0I0I0I00I00I00I00I00I00I00I00I000xi , 0CyK0K0K0K0K0K0K0K00K00K00K0K0K0K0I0I0I0 0z 8?Bv|y '"#}XXXXXXXCXC fl6  f/!  f46  f6  f6 f6 f6 f6 fl6 ff) f4!7 f7 f̉/I|'03>     -66N%.2=AA     >*urn:schemas-microsoft-com:office:smarttags PostalCode9*urn:schemas-microsoft-com:office:smarttagsStateB *urn:schemas-microsoft-com:office:smarttagscountry-region9*urn:schemas-microsoft-com:office:smarttagsplace: *urn:schemas-microsoft-com:office:smarttagsStreet8 *urn:schemas-microsoft-com:office:smarttagsCity;*urn:schemas-microsoft-com:office:smarttagsaddress \{A    $iv+-1bq#EL@ D j w 3 G I ] S W i p v z  VZ PTFM.:'.ALN_  *VZalnqrz  333N@s N@FyN@=RN@dj!N@d/N@0u4N@qDN@"^N@k`N@&&gN@5VnN@sN@lL/uN@,W{N@D@@͕99`@UnknownGz Times New Roman5Symbol3& z Arial7&  Verdana;[SOSimSun qh:f:f`= )`= )!-!),.:;?]}    & 6"0000 0 0 00000 =@\]^([{  0 0 00000;[22HX(?D2wTitle: Polymorphonuclear neutrophils and their mediators in gingival tissues from generalized aggressive periodontitis lxxlxxOh+'0(4D P\ |  xTitle: Polymorphonuclear neutrophils and their mediators in gingival tissues from generalized aggressive periodontitis lxxNormallxx2Microsoft Office Word@Ik@@`=՜.+,D՜.+, X`px tshg)    8@ _PID_HLINKSA*m|http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=EasyRelated&UT=000172411700011&doc=18/1&rec_id=122554589RDmhttp://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=DispCitingRec&doc=18/1&isickref=122554589Rq http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=PagedCitedRefList&UT=000172411700011&doc=18/1&rec_id=122554589R' http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=OneClickSearch&field=AU&val=Gao+Y&curr_doc=18/1&Form=FullRecordPage&doc=18/1R9http://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=OneClickSearch&field=AU&val=Meng+HX&curr_doc=18/1&Form=FullRecordPage&doc=18/1R9Ohttp://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=OneClickSearch&field=AU&val=Cao+CF&curr_doc=18/1&Form=FullRecordPage&doc=18/1R<Khttp://wos15.isiknowledge.com/CIW.cgi?SID=Z2BpEl7c9GkG4GojDN9&Func=OneClickSearch&field=AU&val=Liu+RK&curr_doc=18/1&Form=FullRecordPage&doc=18/1R  !"#$%&'()+,-./013456789<Root Entry F>Data 1Table"WordDocument2*SummaryInformation(*DocumentSummaryInformation82CompObjm  FMicrosoft Office Word ĵ MSWordDocWord.Document.89q